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Analysis of eight water soluble vitamins in ricebean (Vigna umbellata) varieties from NE India by reverse phase-HPLC

  • Rejaul Hoque Bepary
  • D. D. WadikarEmail author
  • P. E. Patki
Original Paper
  • 12 Downloads

Abstract

A simple, quick, and sensitive HPLC procedure for simultaneous quantification of eight water-soluble vitamins (WSVs) in 11 ricebean (Vigna umbellata) varieties (JCR-08-7, JCR-08-8, JCR-08-10, JCR-08-12, JCR-08-15, JCR-08-16, JCR-08-32, JCR-13-11, JCR-13-13, JCR-50 and Nagadal) is described. The method includes enzymatic digestion followed by clean-up with solid phase extraction column and then analysis of extracted compounds by reverse phase column using UV-detector. The optimum conditions revealed that the method had an excellent linearity with good regression coefficient (r > 0.993), precision (0.3–2.77%), and accuracy (83–124%), and relatively short analysis time (13 min). The detection and quantification limits for all WSVs ranged 0.1470–1.1583 µg/mL and 0.4456–3.51 µg/mL, respectively. The average content (mg/100 g) of WSVs in 11 ricebean varieties was—B1: 0.579 ± 0.22, C: 2.111 ± 0.86, B3: 2.292 ± 1.15, B6 (pyridoxal-5-phosphate): 7.002 ± 1.31, B6 (pyridoxine hydrochloride): 2.79 ± 3.23, B5: 6.163 ± 3.35, B9: 0.289 ± 0.14, and B2: 0.163 ± 0.06. Principal components analysis showed that Nagadal belongs to third group which had the highest amount vitamin B1, vitamin B3, vitamin B5, and vitamin B2.

Keywords

Water soluble vitamins HPLC Ricebean Method validation 

Notes

Acknowledgements

The authors acknowledge Ministry of Minority Affairs, Govt. of India, New Delhi and UGC, New Delhi for providing Fellowship to carry out the research works. The authors are gratefully acknowledge the Director, DFRL, Mysore for providing facilities, the support and encouragement to carry out this research and Dr (Mrs) Seuji. Borah Neog, Principal Scientist, AICRP on Forage Crops, Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat for providing the RB varieties for study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rejaul Hoque Bepary
    • 1
  • D. D. Wadikar
    • 1
    Email author
  • P. E. Patki
    • 1
  1. 1.DRDO-Defence Food Research LaboratoryMysoreIndia

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