Brown macroalgae from the Adriatic Sea as a promising source of bioactive nutrients

  • Marijana KosanićEmail author
  • Branislav Ranković
  • Tatjana Stanojković
Original Paper


In the present investigation the acetone extracts of three brown marine macroalgae Dictyota dichotoma, Padaina pavonia and Sargassum vulgare were tested for antioxidant, antimicrobial and cytotoxic potential. The antioxidant activity was evaluated by free radical scavenging, superoxide anion radical scavenging and reducing power. P. pavonia extract showed more potent free radical scavenging activity (IC50 = 691.56 µg L−1) than D. dichotoma and S. vulgare extracts. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. The total content of phenol and flavonoid in extracts was examined using Folin–Ciocalteu reagent and aluminium chloride method and the obtained values were expressed as pyrocatechol equivalents, and as rutin equivalents, respectively. Further, the antimicrobial potential was determined by a microdilution method. Among the tested species, the extract of D. diichotoma showed the best antimicrobial activity with minimum inhibitory concentration values ranging from 0.156 to 2.5 mg L−1. Finally, the cytotoxic activity was tested using microculture tetrazolium test on the human colon carcinoma LS174 cells, human lung carcinoma A549 cells, malignant melanoma FemX cells and chronic myelogenous leukaemia K562 cells. The extract of D. dichotoma expressed stronger cytotoxic activity toward tested cell lines with IC50 values ranging from 9.76 to 50.96 µg L−1.


Acetone extracts Anticancer activity Antimicrobial activity Antioxidant activity Algae 



This work was financed in part by the Ministry of Science, Technology, and Development of the Republic of Serbia and was carried out within the framework of Projects No. 173032 and 175011.

Compliance with ethical standards

Conflict of interest

All contributing authors declare no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marijana Kosanić
    • 1
    Email author
  • Branislav Ranković
    • 1
  • Tatjana Stanojković
    • 2
  1. 1.Department of Biology, Faculty of ScienceUniversity of KragujevacKragujevacSerbia
  2. 2.Institute of Oncology and Radiology of SerbiaBelgradeSerbia

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