Advertisement

In vitro antioxidant and anti-inflammatory activities of Melipona beecheii honey protein fractions

  • Jorge Carlos Ruiz-Ruiz
  • Pablo Acereto-Escoffié
  • Maira Rubí Segura-Campos
Original Paper
  • 54 Downloads

Abstract

Proteins extracted from Melipona beecheii honey were assessed for its antioxidant and anti-inflammatory activity by in vitro assays. Protein content was 3.56 mg/mL and after reverse phase high-performance liquid chromatography fractionation eight distinctive peaks were detected with molecular weight ranged from 7.04 to 95.35 kDa. Low molecular weight fraction constituted by proteins with weights of 7.04, 14.07 and 24.16 kDa, exhibited the highest antioxidant activities with the lowest IC50 values for radical scavenging activity (0.4262 mg/mL), chelating effects (0.3763 mg/mL), and reducing power (0.3060 mg/mL). Same fraction exhibited the lowest IC50 values for inhibition of protein thermal denaturation (0.4115 mg/mL) and cell membrane stabilization (0.5563 mg/mL). For its part, the complete protein extract exhibited the lowest inhibition of proteolytic activity (4.3548 mg/mL). From the results, it is concluded that proteins of honey produced by Melipona beecheii are partially related with its antioxidant and anti-inflammatory activities. These findings validate the use that many Mesoamerican cultures have made of the honey produced by M. beecheii for the healing of lesions and dermic pathologies. Once deeper studies and the pertinent clinical trials have been carried out, the honey of M. beecheii and its proteins could be incorporated into products for pharmaceutical use.

Keywords

Stingless bee Honey Protein fractions Antioxidant Anti-inflammatory 

Notes

Acknowledgements

The authors thank the technical assistance of the Instrumental Analysis Laboratory and the Food Science Laboratory of the Faculty of Chemical Engineering of the Autonomous University of Yucatan.

References

  1. 1.
    J.S.F. Swaran, Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid. Med. Cell. Longev. 2(4), 191–206 (2009)CrossRefGoogle Scholar
  2. 2.
    F.M.C. Ndoye-Foe, T.F. Kemegni-Tchinang, A.M. Nyegue, J.P. Abdou, A.J. Gbaweng-Yaya, A. Tiabou-Tchinda, J.L. Jean-Louis Oyono-Essame, F.X. Etoa, Chemical composition, in vitro antioxidant and anti-inflammatory properties of essential oils of four dietary and medicinal plants from Cameroon. BMC Complement. Altern. Med. 16, 117–129 (2016)CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    M.L. Ferrero, O.H. Nielsen, P.S. Andersen, S.E. Girardin, Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin. Exp. Immunol. 147, 227–235 (2007)Google Scholar
  4. 4.
    N.B. Menke, K.R. Ward, T.M. Witten, D.G. Bonchev, R.F. Diegelmann, Impaired wound healing. Clin. Dermatol. 25, 19–25 (2007)CrossRefPubMedGoogle Scholar
  5. 5.
    A.A. Geronikaki, A.M. Gavalas, Antioxidants and anti-inflammatory diseases: synthetic and natural antioxidants with anti-inflammatory activity. Comb. Chem. High Throughput Screen. 9, 425–442 (2006)CrossRefPubMedGoogle Scholar
  6. 6.
    P.V. Rao, K.T. Krishnana, N. Sallehb, S.H. Ganc, Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Rev. Bras. Farmacogn. 26, 657–664 (2016)CrossRefGoogle Scholar
  7. 7.
    J.M. Alvarez-Suarez, S. Tulipani, S. Romandini, E. Bertoli, M. Battino, Contribution of honey in nutrition and human health: a review. Mediterr. J. Nutr. Metab. 3(1), 15–23 (2010)CrossRefGoogle Scholar
  8. 8.
    J.M. Alvarez-Suarez, F. Giampieri, A. Brenciani, M. Battino, Apis mellifera vs Melipona beecheii Cuban polifloral honeys: a comparison based on their physicochemical parameters, chemical composition and biological properties. LWT-Food Sci. Technol. 89, 272–279 (2017)Google Scholar
  9. 9.
    L.B. Almeida-Muradian, K.M. Stramm, L.M. Estevinho, Efficiency of the FTIR ATR spectrometry for the prediction of the physicochemical characteristics of Melipona subnitida honey and study of the temperature’s effect on those properties. Int. J. Food Sci. Technol. 49, 188–195 (2014)CrossRefGoogle Scholar
  10. 10.
    M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 24248–24254 (1976)CrossRefGoogle Scholar
  11. 11.
    D. Bizani, A. Motta, J. Morrissy, R. Terra, A.Y. Souto, A. Brandelli, Antibacterial activity of cerein 8A, a bacteriocin-like peptide produces by Bacillus cereus. Int. Microbiol. 8, 125–131 (2005)PubMedGoogle Scholar
  12. 12.
    A. Saiga, S. Tanabe, T. Nishimura, Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. J. Agric. Food Chem. 51, 3661–3667 (2003) (2003)CrossRefPubMedGoogle Scholar
  13. 13.
    G.C. Yen, H.Y. Chen, Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43, 27–32 (1995)CrossRefGoogle Scholar
  14. 14.
    Y. Mizushima, M. Kobayashi, Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharm. Pharmacol. 20, 169–173 (1968)CrossRefPubMedGoogle Scholar
  15. 15.
    J. Sadique, W.A. Al-Rqobahs, E.I. Bughaith, A.R. Gindi, The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia 60, 525-532 (1089)Google Scholar
  16. 16.
    U.A. Shinde, A.S. Phadke, A.M. Nari, A.A. Mungantiwar, V.J. Dikshit, M.N. Saraf, Membrane stabilization activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 70, 251-257 (1999)Google Scholar
  17. 17.
    O.O. Oyedepo, A.J. Femurewa, Anti-protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int. J. Pharmacogn. 33, 65-69 (1995)Google Scholar
  18. 18.
    D.C. Montgomery, Diseño y análisis de experimentos. 2da Ed. México, D.F: Editorial Limusa S.A. de C.V., pp. 21–141 (2007)Google Scholar
  19. 19.
    J.A. Nweze, J.I. Okafor, I. Emeka. E.I. Nweze, J.E. Nweze, Evaluation of physicochemical and antioxidant properties of two stingless bee honeys: a comparison with Apis mellifera honey from Nsukka, Nigeria. BMC Res. Notes 10, 566 (2017)CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    M.F. Abu Bakar, S.B. Sanusi, F.I. Abu Bakar, O.J. Cong, Z. Mian, Physicochemical and antioxidant potential of raw unprocessed honey from Malaysian stingless bees. Pak. J. Nutr. 16(11), 888–894 (2017)CrossRefGoogle Scholar
  21. 21.
    M. Abd El Aal, S. Zalat, A. Abouzeid, A. Ibrahim, Protein pattern of the honeybee venoms of Egypt. Egypt. J. Biol. 4, 42–46 (2002)Google Scholar
  22. 22.
    S.A. Mohammed, E. Babiker, Protein structure, physicochemical properties and mineral composition of Apis mellifera honey samples of different floral origin. Aust. J. Basic Appl. Sci. 3(3), 2477–2483 (2009)Google Scholar
  23. 23.
    J. Danihlík, K. Aronstein, M. Petřivalský, Antimicrobial peptides: a key component of honey bee innate immunity. J. Apic. Res. 54(2), 123–136 (2015)CrossRefGoogle Scholar
  24. 24.
    R.J. Elias, S.S. Kellerby, E.A. Decker, Antioxidant activity of proteins and peptides. Crit. Rev. Food Sci. Nutr. 48(5), 430–441 (2008)CrossRefPubMedGoogle Scholar
  25. 25.
    M. Diaz, C.M. Dunn, D.J. McClements, E.A. Decker, Use of caseinophosphopeptides as natural antioxidants in oil-in-water emulsions. J. Agric. Food Chem. 51, 2365–2370 (2003)CrossRefPubMedGoogle Scholar
  26. 26.
    G. Leelaprakash, S. Mohan-Dass, In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int. J. Drug Dev. Res. 3(3), 189–196 (2011)Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jorge Carlos Ruiz-Ruiz
    • 1
  • Pablo Acereto-Escoffié
    • 2
  • Maira Rubí Segura-Campos
    • 2
  1. 1.Escuela de Nutrición, División de Ciencias de la SaludUniversidad Anáhuac-MayabMéridaMexico
  2. 2.Facultad de Ingeniería QuímicaUniversidad Autónoma de YucatánMéridaMexico

Personalised recommendations