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Frontiers of Medicine

, Volume 13, Issue 3, pp 330–343 | Cite as

Overexpressed long noncoding RNA CRNDE with distinct alternatively spliced isoforms in multiple cancers

  • Xuefei Ma
  • Wei Zhang
  • Rong Zhang
  • Jingming Li
  • Shufen Li
  • Yunlin Ma
  • Wen JinEmail author
  • Kankan WangEmail author
Research Article

Abstract

Alternative splicing is a tightly regulated process that contributes to cancer development. CRNDE is a long noncoding RNA with alternative splicing and is implicated in the pathogenesis of several cancers. However, whether deregulated expression of CRNDE is common and which isoforms are mainly involved in cancers remain unclear. In this study, we report that CRNDE is aberrantly expressed in the majority of solid and hematopoietic malignancies. The investigation of CRNDE expression in normal samples revealed that CRNDE was expressed in a tissue- and cell-specific manner. Further comparison of CRNDE expression in 2938 patient samples from 15 solid and hematopoietic tumors showed that CRNDE was significantly overexpressed in 11 malignancies, including 3 reported and 8 unreported, and also implicated that the overexpressed isoforms differed in various cancer types. Furthermore, anti-cancer drugs could efficiently repress CRNDE overexpression in cancer cell lines and primary samples, and even had different impacts on the expression of CRNDE isoforms. Finally, experimental profiles of 12 alternatively spliced isoforms demonstrated that the spliced variant CRNDE-g was the most highly expressed isoform in multiple cancer types. Collectively, our results emphasize the cancer-associated feature of CRNDE and its spliced isoforms, and may provide promising targets for cancer diagnosis and therapy.

Keywords

long noncoding RNA CRNDE alternative splicing 

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Notes

Acknowledgements

This work was supported in part by National Natural Science Foundation of China (Nos. 81530003, 81300403, 81770153 and 91440114), The National Key Research and Development Program (No. 2016YFC0902800), Shanghai Leading Talent Projects (No. 2015008) and the Academic Leader Program of Shanghai Science and Technology Committee (No. 2015137).

Supplementary material

11684_2017_557_MOESM1_ESM.pdf (773 kb)
Supplementary material, approximately 772 KB.

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Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Medical Genomics and Shanghai Institute of HematologyRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina
  3. 3.Department of HematologyXi’an Gaoxin HospitalXi’anChina

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