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Overexpression of PtPEPCK1 gene promotes nitrogen metabolism in poplar

  • Lina WangEmail author
  • Miao He
  • Song Chen
  • Kean Wang
  • Donghai Cui
  • Xin Huang
  • Lijie Liu
Original Paper
  • 26 Downloads

Abstract

To understand the function of phosphoenolpyruvate carboxylase kinase, we introduced PtPEPCK1 gene under the control of 35S promoter into 84K poplar (Populus alba × P. glandulosa). PtPEPCK1 gene is well-known for its role in gluconeogenesis. However, our data confirmed that it has significant effects on amino acid biosynthesis and nitrogen metabolism. Immunohistochemistry and fluorescence microscopy indicate that PtPEPCK1 is specifically expressed in the cytoplasm of the spongy and palisade tissues. Overexpression of PtPEPCK1 was characterized through transcriptomics and metabolomics. The metabolites concentration of the ornithine cycle and its precursors also increased, of which N-acetylornithine was up-regulated almost 50-fold and ornithine 33.7-fold. These were accompanied by a massive increase in levels of several amino acids. Therefore, overexpression of PtPEPCK1 increases amino acid levels with urea cycle disorder.

Keywords

Amino acid metabolism Metabolome Nitrogen metabolism Phosphoenolpyruvate carboxylase kinase Transcriptome Urea cycle 

Notes

Supplementary material

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Supplementary material 1 (DOCX 18 kb)
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Supplementary material 4 (DOCX 15 kb)

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Copyright information

© Northeast Forestry University 2019

Authors and Affiliations

  • Lina Wang
    • 1
    Email author
  • Miao He
    • 1
  • Song Chen
    • 1
  • Kean Wang
    • 1
  • Donghai Cui
    • 1
  • Xin Huang
    • 2
  • Lijie Liu
    • 2
  1. 1.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinPeople’s Republic of China
  2. 2.College of Life Sciences and Agriculture and ForestryQiqihar UniversityQiqiharPeople’s Republic of China

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