Over expression of TaFer gene from Tamarix androssowii improves iron and drought tolerance in transgenic Populus tomentosa
- 80 Downloads
Ferritin, a universal intracellular protein, can store large amounts of iron and improve plant resistance to abiotic and biotic stress. In this study, a ferritin gene (TaFer) from Tamarix androssowii Litv. was transferred into Populus tomentosa Carr. cv ‘BJR01’ via Agrobacterium. Six independent transgenic lines were obtained with a tolerance to kanamycin and three were randomly selected for further analysis. The PCR and RT-PCR results indicate that the TaFer gene had been integrated into the poplar genome. The effect of the gene on abiotic stress tolerance was tested, and the results show that transgenic plants improve growth, had higher chlorophyll and lower MDA contents, and higher relative electrical conductivity, fewer changes of SOD and POD activities, higher iron content, higher root ferric reductase activity and lower levels of ROS accumulation and cell death in response to drought, Fe-insufficient or Fe-excess tolerance. These results indicate that the TaFer gene can improve abiotic stress tolerance in transgenic Populus tomentosa.
KeywordsTarmarix andnssowii Ferritin Iron Genetic transformation Stress resistance
- Briat JF (2002) Metal iron mediated oxidative stress and its control. In: Montagu M, Inzé D (eds) Oxidative stress in plants. Taylor and Francis, London, pp 171–189Google Scholar
- Hideg É, Török K, Šnyrychová I, Sándor G, Szegedi E, Horváth VG (2007) Response of ferritin over-expressing tobacco plants to oxidative stress, vol 91. Springer, Berlin, pp 1469–1472Google Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar