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Simple Label-Free DNA Sensor Based on CeO2 Nanorods Decorated with Ppy Nanoparticles

  • Nguyen Thi Nguyet
  • Vu Van Thu
  • Hoang Lan
  • Tran Trung
  • Anh-Tuan Le
  • Vuong-Hung PhamEmail author
  • Phuong Dinh TamEmail author
Article
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Abstract

A facile label-free DNA sensor based on cerium oxide nanorods decorated with polypyrrole nanoparticles (CeO2-NRs/Ppy-NPs) matrix has been developed for detection of Salmonella. The sensor was fabricated by hydrothermal synthesis of CeO2-NRs on the microelectrode surface followed by in situ chemical oxidative polymerization of pyrrole on the CeO2-NRs to prepare a CeO2-NR/Ppy-NPs electrode. Single-stranded DNA (ssDNA) sequences were immobilized onto the modified microelectrode by covalent attachment. The properties of the material were explored by field-emission scanning electron microscopy, x-ray diffraction analysis, and Fourier-transform infrared spectroscopy techniques. The response of the DNA biosensor was investigated by electrochemical impedance spectroscopy with [Fe(CN)6]3−/4− as redox probe. The results showed that the response of the DNA biosensor exhibited good linearity within the range of 1.0 × 10−9 mol L−1 to 1.0 × 10−6 mol L−1 with sensitivity of 14.7 × 106 Ω/mol L−1 cm−1. The limit of detection and limit of quantification of the DNA biosensor were low, with values of 2.86.10−7 mol L−1 and 9.56.10−7 mol L−1, respectively.

Keywords

Nanocomposite conducting polymer biosensor nanorod CeO2 

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References

  1. 1.
    H. Li, U.B. Gyllensten, X. Cui, R.K. Saiki, H.A. Erlich, and N. Arnheim, Nature 335, 414 (1988).CrossRefGoogle Scholar
  2. 2.
    Y.Q. Du, P.F. Gao, W. Wang, T.T. Wang, Y. Chang, J. Wang, and ChZh Huang, Analyst 138, 5745 (2013).CrossRefGoogle Scholar
  3. 3.
    R.P. Stokes, A. Cordwell, and R.A. Thompson, J. Clin. Pathol. 35, 566 (1982).CrossRefGoogle Scholar
  4. 4.
    C.S. Riccardi, H. Yamanaka, M. Josowicz, J. Kowalik, B. Mizaikoff, and C. Kranz, Anal. Chem. 78, 1139 (2006).CrossRefGoogle Scholar
  5. 5.
    Y. Guo, J.H. Chen, and C. Guonan, Sens. Actuator B-Chem. 184, 113 (2013).CrossRefGoogle Scholar
  6. 6.
    C. Singhal, M. Khanuja, N. Chaudhary, C.S. Pundir, and J. Narang, Sci. Rep. 8, 7734 (2018).CrossRefGoogle Scholar
  7. 7.
    Z. Liu and X. Su, Biosens. Bioelectron. 87, 66 (2017).CrossRefGoogle Scholar
  8. 8.
    M. Mehrgardi and L. Ahangar, Biosens. Bioelectron. 26, 4308 (2011).CrossRefGoogle Scholar
  9. 9.
    C.P.S. Liew, B. Lertanantawong, S.Y. Lee, R. Manickam, Y.H. Lee, and W.S. Chai, Talanta 139, 167 (2015).CrossRefGoogle Scholar
  10. 10.
    G. Martínez-Paredes, M.B. González-García, and A. Costa-García, Sens. Actuator B-Chem. 149, 329 (2010).CrossRefGoogle Scholar
  11. 11.
    A.C. Lee, D. Du, B. Chen, C.K. Heng, T.M. Lim, and Y. Lin, Analyst 139, 4223 (2014).CrossRefGoogle Scholar
  12. 12.
    M. Asghary, J.B. Raoof, M. Rahimnejad, and R. Ojani. Biosens. Bioelectron. 82, 173 (2016).CrossRefGoogle Scholar
  13. 13.
    A. Liu, K. Wang, S. Weng, Y. Lei, K. Lin, W. Chen, X. Lin, and Y. Chen, Trends Anal. Chem. 37, 101 (2012).CrossRefGoogle Scholar
  14. 14.
    M. Mohammed, I.J. Ibraheem, A.S. Obaid, and M. Bououdina, Sens Biosensing Res. 15, 46 (2017).CrossRefGoogle Scholar
  15. 15.
    P.A. Rasheed and N. Sandhyarani, Biosens. Bioelectron. 15, 226 (2017).CrossRefGoogle Scholar
  16. 16.
    S. Peschel, B. Ceyhan, C.M. Niemeyer, S. Gao, L. Chi, and U. Simon, Mater. Sci. Eng., C 19, 47 (2002).CrossRefGoogle Scholar
  17. 17.
    W. Gao, X. Wei, X. Wang, G. Cui, Z. Liua, and B. Tang, Chem. Commun. 52, 3643 (2016).CrossRefGoogle Scholar
  18. 18.
    N.T. Nguyet, L.T.H. Yen, V.V. Thu, H. Lan, T. Trung, P.H. Vuong, and P.D. Tam, J. Phys. Chem. Solids 115, 18 (2018).CrossRefGoogle Scholar
  19. 19.
    M.B. Gumpu, N. Nesakumar, S. Sethuraman, U.M. Krishnan, and J.B.B. Rayappan, Sens. Actuators B –Chem. 199, 330 (2014).CrossRefGoogle Scholar
  20. 20.
    M. Singh, N. Nesakumar, S. Sethuraman, U.M. Krishnan, and J.B.B. Rayappan, J. Colloid Interface Sci. 425, 52 (2014).CrossRefGoogle Scholar
  21. 21.
    Q. Wang, F. Gao, X. Zhang, B. Zhang, S. Li, Z. Hu, and F. Gao, Electrochim. Acta 62, 250 (2012).CrossRefGoogle Scholar
  22. 22.
    W. Zhang, T. Yang, X. Zhuang, Z. Guo, and K. Jiao, Biosens. Bioelectron. 24, 2417 (2009).CrossRefGoogle Scholar
  23. 23.
    X. Qian, Q. Qu, L. Li, X. Ran, L. Zuo, R. Huang, and Q. Wang, Sensors 18, 1878 (2018).CrossRefGoogle Scholar
  24. 24.
    U. Baig, M.A. Gondal, A.M. Ilyas, and M.M. Sanagi, J. Mater. Sci. Technol. 33, 547 (2017).CrossRefGoogle Scholar
  25. 25.
    S. Peshoria and A.K. Narula, J. Mater. Sci. 53, 3876 (2018).CrossRefGoogle Scholar
  26. 26.
    M. Bruck, C.N. Gannett, D.C. Bock, P.F. Smith, A.C. Marschilok, K.J. Takeuchi, and E.S. Takeuchi, J. Electrochem. Soc. 164, A6260 (2017).CrossRefGoogle Scholar
  27. 27.
    J. Zhang and X.S. Zhao, J. Phys. Chem. C 116, 5420 (2012).CrossRefGoogle Scholar
  28. 28.
    F. Li, X. Han, and S. Liu, Biosens. Bioelectron. 26, 2619 (2011).CrossRefGoogle Scholar
  29. 29.
    M. Singh, N. Nesakumar, S. Sethuraman, U.M. Krishnan, and J.B.B. Rayappan, J. Colloid Interface Sci. 425, 52 (2014).CrossRefGoogle Scholar
  30. 30.
    X.C. Zhou, L.Q. Huang, and S.F.Y. Li, Biosens. Bioelectron. 16, 85 (2001).CrossRefGoogle Scholar
  31. 31.
    Y. Okahata, M. Kawase, K. Niilura, F. Ohtake, H. Furusawa, and Y. Ebara, Anal. Chem. 70, 1288 (1998).CrossRefGoogle Scholar
  32. 32.
    P.D. Tam, M.A. Tuan, N.V. Hieu, and N.D. Chien, Physica E 41, 1567 (2009).CrossRefGoogle Scholar
  33. 33.
    S. Surzycki, Human Molecular Biology Laboratory Manual (Wiley - Blackwell, 2003), ISBN 9780632046768..Google Scholar

Copyright information

© The Minerals, Metals & Materials Society 2019

Authors and Affiliations

  1. 1.Advanced Institute for Science and TechnologyHanoi University of Science and TechnologyHanoiVietnam
  2. 2.Faculty of Material Science and EngineeringPhenikaa UniversityHanoiVietnam
  3. 3.Hung Yen University of Technology and EducationHung YenVietnam
  4. 4.Faculty of Occupational Safety and HealthTrade Union UniversityHanoiVietnam
  5. 5.Phenikaa University Nano InstitutePhenikaa UniversityHanoiVietnam

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