Simple Label-Free DNA Sensor Based on CeO2 Nanorods Decorated with Ppy Nanoparticles

  • Nguyen Thi Nguyet
  • Vu Van Thu
  • Hoang Lan
  • Tran Trung
  • Anh-Tuan Le
  • Vuong-Hung PhamEmail author
  • Phuong Dinh TamEmail author


A facile label-free DNA sensor based on cerium oxide nanorods decorated with polypyrrole nanoparticles (CeO2-NRs/Ppy-NPs) matrix has been developed for detection of Salmonella. The sensor was fabricated by hydrothermal synthesis of CeO2-NRs on the microelectrode surface followed by in situ chemical oxidative polymerization of pyrrole on the CeO2-NRs to prepare a CeO2-NR/Ppy-NPs electrode. Single-stranded DNA (ssDNA) sequences were immobilized onto the modified microelectrode by covalent attachment. The properties of the material were explored by field-emission scanning electron microscopy, x-ray diffraction analysis, and Fourier-transform infrared spectroscopy techniques. The response of the DNA biosensor was investigated by electrochemical impedance spectroscopy with [Fe(CN)6]3−/4− as redox probe. The results showed that the response of the DNA biosensor exhibited good linearity within the range of 1.0 × 10−9 mol L−1 to 1.0 × 10−6 mol L−1 with sensitivity of 14.7 × 106 Ω/mol L−1 cm−1. The limit of detection and limit of quantification of the DNA biosensor were low, with values of 2.86.10−7 mol L−1 and 9.56.10−7 mol L−1, respectively.


Nanocomposite conducting polymer biosensor nanorod CeO2 


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Copyright information

© The Minerals, Metals & Materials Society 2019

Authors and Affiliations

  1. 1.Advanced Institute for Science and TechnologyHanoi University of Science and TechnologyHanoiVietnam
  2. 2.Faculty of Material Science and EngineeringPhenikaa UniversityHanoiVietnam
  3. 3.Hung Yen University of Technology and EducationHung YenVietnam
  4. 4.Faculty of Occupational Safety and HealthTrade Union UniversityHanoiVietnam
  5. 5.Phenikaa University Nano InstitutePhenikaa UniversityHanoiVietnam

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