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Chinese Journal of Integrative Medicine

, Volume 25, Issue 12, pp 939–947 | Cite as

Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis

  • Guang ZhengEmail author
  • He Zhang
  • Yun Yang
  • Ying-li Sun
  • Yan-jing Zhang
  • Ju-ping Chen
  • Ting Hao
  • Cheng Lu
  • Hong-tao Guo
  • Ge Zhang
  • Dan-ping Fan
  • Xiao-juan He
  • Ai-ping Lu
Interdisciplinary Knowledge

Abstract

Objective

To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis.

Methods

This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments.

Results

The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated.

Conclusion

This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.

Keywords

Angelicae sinensis Radix interactome cell proliferation hemopoiesis regulation cross-talking agents 

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Notes

Acknowledgements

The authors thank GUO Bo-sheng, and HE Bing for their biomedical knowledge and beneficial discussions provided in the process of data pretreatments.

Author Contributions

Zheng G and Lu AP are responsible for the construction and analysis on interactome. He XJ and Fan DP are responsible for the experimental validation. Zhang H, Yang Y, Zhang YJ, Chen JP, and Hao T are responsible for data downloading, data format converting, data integrating, and double checking. Lu C, Guo HT, Sun YL and Zhang G are responsible for the biological analysis on interactome e.g., protein tissue expression, compound target protein, internal protein action, and overrepresented biological process. All authors read and approved the final manuscript.

Supplementary material

11655_2018_3003_MOESM1_ESM.docx (942 kb)
Supplementary material, approximately 941 KB.

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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Guang Zheng
    • 1
    Email author
  • He Zhang
    • 1
  • Yun Yang
    • 1
  • Ying-li Sun
    • 2
  • Yan-jing Zhang
    • 1
  • Ju-ping Chen
    • 1
  • Ting Hao
    • 1
  • Cheng Lu
    • 3
  • Hong-tao Guo
    • 4
  • Ge Zhang
    • 5
  • Dan-ping Fan
    • 3
  • Xiao-juan He
    • 3
  • Ai-ping Lu
    • 5
  1. 1.Information Science and Engineering SchoolLanzhou UniversityLanzhouChina
  2. 2.School of BiologyLanzhou UniversityLanzhouChina
  3. 3.Institute of Basic Research in Chinese MedicineChina Academy of Chinese Medical SciencesBeijingChina
  4. 4.Department of Rheumatismthe First Affiliated Hospital of Henan University of Traditional Chinese MedicineZhengzhouChina
  5. 5.School of Chinese MedicineHong Kong Baptist UniversityKowloon Tong, Kowloon, Hong Kong SARChina

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