Systems-Based Interactome Analysis for Hematopoiesis Effect of Angelicae sinensis Radix: Regulated Network of Cell Proliferation towards Hemopoiesis
- 41 Downloads
To explore the molecular-level mechanism on the hematopoiesis effect of Angelicae sinensis Radix (ASR) with systems-based interactome analysis.
This systems-based interactome analysis was designed to enforce the workflow of "ASR (herb)→compound→target protein→internal protein actions→ending regulated protein for hematopoiesis". This workflow was deployed with restrictions on regulated proteins expresses in bone marrow and anemia disease and futher validated with experiments.
The hematopoiesis mechanism of ASR might be accomplished through regulating pathways of cell proliferation towards hemopoiesis with cross-talking agents of spleen tyrosine kinase (SYK), Janus kinase 2 (JAK2), and interleukin-2-inducible T-cell kinase (ITK). The hematopoietic function of ASR was also validated by colony-forming assay performed on mice bone marrow cells. As a result, SYK, JAK2 and ITK were activated.
This study provides a new approach to systematically study and predict the therapeutic mechanism for ASR based on interactome analysis towards biological process with experimental validations.
KeywordsAngelicae sinensis Radix interactome cell proliferation hemopoiesis regulation cross-talking agents
Unable to display preview. Download preview PDF.
The authors thank GUO Bo-sheng, and HE Bing for their biomedical knowledge and beneficial discussions provided in the process of data pretreatments.
Zheng G and Lu AP are responsible for the construction and analysis on interactome. He XJ and Fan DP are responsible for the experimental validation. Zhang H, Yang Y, Zhang YJ, Chen JP, and Hao T are responsible for data downloading, data format converting, data integrating, and double checking. Lu C, Guo HT, Sun YL and Zhang G are responsible for the biological analysis on interactome e.g., protein tissue expression, compound target protein, internal protein action, and overrepresented biological process. All authors read and approved the final manuscript.
- 2.Ji P, Wei Y, Hua Y, Zhang X, Yao W, Ma Q, et al. A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of unprocessed Angelica sinensis and its 4 kinds of processed products. J Ethnopharmacol 2018;211:101–116.PubMedCrossRefPubMedCentralGoogle Scholar
- 3.Zhou Z, ed. Internal medicine of traditional Chinese medicine. Beijing: China Press of Traditional Chinese Medicine; 2007.Google Scholar
- 4.Xiao H, Xiong L, Song X, Jin P, Chen L, Chen X, et al. Angelica sinensis polysaccharides ameliorate stressinduced premature senescence of hematopoietic cell via protecting bone marrow stromal cells from oxidative injuries caused by 5-fluorouracil. Int J Mol Sci 2017;18. pii: E2265.Google Scholar
- 29.Protein analysis through evolutionary relationships. Available at: http://pantherdb.org/. Accessed 29 June, 2016.
- 40.Xiao ZH, He F, Yao HL, Han JS, Liu ZW. Research of ITK regulation on mouse spleen lymphocytes proliferation and differentiation. Chin J Exp Clin Virol (Chin) 2009,23:269–271.Google Scholar
- 51.Iyer AS, Morales JL, Huang W, Ojo F, Ning G, Wills E, et al. Absence of Tec family kinases interleukin-2 inducible T cell kinase (Itk) and Bruton’s tyrosine kinase (Btk) severely impairs Fc epsilonRI-dependent mast cell responses. J Biol Chem 2011;286:9503–9513.PubMedPubMedCentralCrossRefGoogle Scholar