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Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating the Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats

  • Xiao Kang
  • Hua-jun ZhouEmail author
  • Jian Yang
  • Jian-hua Zhong
  • Tao Tang
  • Han-jin Cui Cui
  • Jing-hua Zhou
  • Zhi-gang Mei
Original Article
  • 17 Downloads

Abstract

Objective

To evaluate the effect of Buyang Huanwu Decoction (补阳还五汤, BYHWD) on glial scar after intracerebral hemorrhage (ICH) and investigate the underlying mechanism.

Methods

Collagenase type VII (0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model. One hundred and twenty Sprague-Dawley rats were randomly divided into 3 groups according to a random number table, including normal group (n=40), ICH model group (n=40) and BYHWD group (n=40), respectively. After ICH, the rats in the BYHWD group were intragastrically administered with BYHWD (4.36 g/kg) once a day for 21 days, while the rats in ICH group were administered with equal volume of distilled water for 21 days, respectively. Double immunolabeling was performed for proliferating cell nuclear antigen (PCNA)+/glial fifibrillary acidic protein (GFAP)+ nuclei. The expression of GFAP and leukemia inhibitory factor (LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).

Results

The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH. The number of GFAP positive cells and GFAP mRNA levels increased notably on day 3 and reached the peak on day 14 post-ICH (P<0.01). PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH (P<0.01). In addition, LIF-positive astrocytes and LIF mRNA level in the hemorrhagic region increased signifificantly till day 14 post-ICH (P<0.01). However, BYHWD not only reduced the number of PCNA+/GFAP+ nuclei, but also decreased GFAP and LIF levels (P<0.05).

Conclusions

BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.

Keywords

Buyang Huanwu Decoction intracerebral hemorrhage glial scar leukemia inhibitory factor Chinese medicine 

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2018

Authors and Affiliations

  • Xiao Kang
    • 1
    • 2
  • Hua-jun Zhou
    • 1
    • 2
    • 3
    Email author
  • Jian Yang
    • 3
  • Jian-hua Zhong
    • 4
  • Tao Tang
    • 5
  • Han-jin Cui Cui
    • 5
  • Jing-hua Zhou
    • 1
    • 2
  • Zhi-gang Mei
    • 6
  1. 1.Institute of NeurologyChina Three Gorges UniversityYichang, Hubei ProvinceChina
  2. 2.Department of Neurology, The First College of Clinical Medical SciencesChina Three Gorges UniversityYichang, Hubei ProvinceChina
  3. 3.Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational MedicineChina Three Gorges UniversityYichang, Hubei ProvinceChina
  4. 4.Department of Intensive Care Unit, The First College of Clinical Medical SciencesChina Three Gorges UniversityYichang, Hubei ProvinceChina
  5. 5.Institute of Integrative Medicine, Xiangya HospitalCentral South UniversityChangshaChina
  6. 6.Medical College of China Three Gorges UniversityYichang, Hubei ProvinceChina

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