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Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells

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Abstract

Objective

To determine the effect of medicated serum of Chinese herbal compound Naofucong (脑复聪, NFC) on the microglia BV-2 cells viability and the transcription and expression of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in microglia BV-2 cells to further explore the mechanisms underlying the protective effect of NFC on inflammatory process induced by high glucose.

Methods

The microglia BV-2 cells incubated in vitro were divided into different groups: the control group (25 mmol/L glucose), the model group (75 mmol/L glucose), high glucose media containing different dose medicated serum of NFC. After being cultured for 24 h, changes in IL-6 and TNF-α were measured by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. The expression of surface marker CD11b of activated microglia was measured by confocal laser scanning microscope and Western blot. Nuclear factor-κB (NF-κB) p-p65 expression was analyzed by Western blot.

Results

The model group obviously increased the expression of microglial surface marker CD11b and NF-κB p-p65 (all P<0.01), induced a signifificant up-regulation of release and the mRNA expression of IL-6 and TNF-α (P<0.01 or P<0.05). The medicated serum of NFC could obviously down-regulate the transcription and expression of surface marker CD11 b and NF-κB p-p65 (all P<0.01), and inhibit the mRNA and protein expression (P<0.01 or P<0.05) of inflflammatory cytokines, such as IL-6 and TNF-α, in microglia BV-2 cells cultured with high glucose for 24 h.

Conclusions

The inhibition of microglial activation and IL-6 and TNF-α expression induced by high glucose may at least partly explain NFC therapeutic effects on diabetes-associated cognitive decline diseases. Its underlying mechanism could probably be related to the inhibition of NFC on NF-κB phosphorylation.

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China

    Guang-chan Jing  (景光婵) & Meng-ren Zhang  (张孟仁)

  2. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China

    Chao Ji  (纪 超), Ping-ping Zuo  (左萍萍), Yu-qin Liu  (刘玉琴) & Bei Gu  (顾 蓓)

Authors
  1. Guang-chan Jing  (景光婵)
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  2. Meng-ren Zhang  (张孟仁)
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  4. Ping-ping Zuo  (左萍萍)
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  6. Bei Gu  (顾 蓓)
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Correspondence to Meng-ren Zhang  (张孟仁).

Additional information

Supported by Beijing Natural Science Foundation (No. 7142129), and Beijing Science and Technology Project of Traditional Chinese Medicine (No. JJ2014-50), China

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Jing, Gc., Zhang, Mr., Ji, C. et al. Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells. Chin. J. Integr. Med. 22, 832–839 (2016). https://doi.org/10.1007/s11655-016-2256-0

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  • DOI: https://doi.org/10.1007/s11655-016-2256-0

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