Direct somatic embryogenesis and shoot regeneration from leaves and internodes of Pluchea lanceolata (DC.) C.B. Clarke

  • Mafatlal M. Kher
  • M. NatarajEmail author
Short Protocol Communication


Pluchea lanceolata (DC.) C.B. Clarke is a threatened native medicinal plant. Increasing the propagation of this plant will preserve the wild population and provide material for medicinal use. In vitro and field-collected shoots and leaves were tested for response to 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), for initiation of direct shoot regeneration (DSR), or direct somatic embryogenesis (DSE). Leaves and internodes collected from field-grown plants produced only callus, while in vitro–raised shoots exhibited DSR and DSE on Murashige and Skoog (MS) medium with 2,4-D and TDZ. Direct shoot regeneration occurred on medium with TDZ from internode and leaf segments obtained from in vitro–developed shoots. In vitro–grown shoots were rooted on half-strength MS medium with 2 mg L−1 indole-3-butyric acid and acclimatized. Survival in natural conditions was 62.5% for DSE and 79% for DSR plantlets.


Adventitious shoots Pluchea lanceolata Shoot buds Somatic embryo 


Author contribution

Conceived and designed the experiments: MMK and MN. Trials conducted by MMK. Wrote the paper, analyzed the data: MMK and MN.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.P.G. Department of BiosciencesSardar Patel UniversityVallabh VidyanagarIndia
  2. 2.School of ScienceGSFC UniversityVadodaraIndia

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