Growth and development of carnation ‘Dreambyul’ plantlets in a temporary immersion system and comparisons with conventional solid culture methods
- 38 Downloads
The aim of the current study was to compare the effects of the culture method—conventional solid medium culture and temporary immersion system (TIS)—on the growth and development of carnation ‘Dreambyul’ plantlets. At the same time, different immersion intervals and immersion durations of TIS culture were also tested to find the optimal setting for mass production of high-quality carnation plantlets in vitro. In the first experiment, the results showed that the shoot length, root length, and number of nodes of plantlets cultured in the TIS were highest when the immersion interval was 8 h. Compared with that of plantlets cultured in the conventional solid medium culture, the fresh weight of plantlets cultured in the TIS was at least 3 times greater. The greatest total chlorophyll content, stomata with normal shapes was observed for plantlets grown in the TIS with an 8-h immersion interval. The lowest H2O2 level was recorded in plantlets cultured with the 8-h immersion interval. In the second study, growth traits such as the shoot length, root length, and stem diameter, as well as the number of shoots and roots tended to increase with immersion durations, and reached their peaks when the immersion duration was 90 s. Excessive water accumulation in tissues and a higher incidence of hyperhydricity were observed in plantlets where the immersion duration was 120 and 150 s. These findings suggest that an immersion interval of 8 h, combined with an immersion duration of 90 s, could be the optimal setting for growth and development of carnation ‘Dreambyul’ plantlets cultured in the TIS.
KeywordsCarnation Dianthus caryophyllus Micropropagation Temporary immersion system
This research was supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project no. 01090805), Rural Development Administration, the Republic of Korea. Luc The Thi was supported by scholarship from the BK21 Plus Program, and the Ministry of Education.
- Afreen F (2006) Temporary immersion bioreactor. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, pp 187–201Google Scholar
- Debergh P, Maene L (1984) Pathological and physiological problems related to the in vitro culture of plants. Parasitica 40:69–75Google Scholar
- Hoang NN, Kitaya Y, Morishita T, Endo R, Shibuya T (2017) A comparative study on growth and morphology of wasabi plantlets under the influence of the micro-environment in shoot and root zones during photoautotrophic and photomixotrophic micropropagation. Plant Cell Tissue Organ Cult 130:255–263CrossRefGoogle Scholar
- Kanwar JK, Kumar S (2010) Effect of growth regulators, explants and their interaction on shoot regeneration in carnation. Adv Hortic Sci 24:115–121Google Scholar
- Levin R, Vasil IK (1989) An integrated and automated tissue culture system for mass propagation of plants. In Vitro Cell Dev Biol-Plant 25:21–27Google Scholar
- Mc Alister B, Finnie J, Watt MP, Blakeway F (2005) Use of the temporary immersion bioreactor system (RITA®) for production of commercial Eucalyptus clones in Mondi Forests (SA). In: Hvoslef-Eide AK, Preil W (eds) Liquid culture systems for in vitro plant propagation. Springer, Dordrecht, pp 425–442CrossRefGoogle Scholar
- Park JE, Park YG, Thi LT, Soundararajan P, Jeong BR (2018) Effect of sucrose concentration, photosynthetic photon flux density, and CO2 concentration on growth and development of micropropagated mountain ash. Propag Ornam Plants 18:58–63Google Scholar
- Stanly C, Bhatt A, Keng CL (2010) A comparative study of Curcuma zedoaria and Zingiber zerumbet plantlet production using different micropropagation systems. Afr J Biotechnol 9:4326–4333Google Scholar
- Ziv M (2000) Bioreactor technology for plant micropropagation. Hortic Rev 24:1–30Google Scholar