Maize seedlings produced from dry seeds exposed to liquid nitrogen display altered levels of shikimate pathway compounds
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In light of climate change and risks of food insecurity, it is becoming increasingly important to preserve plant germplasm in genebanks. Storage of seeds, particularly via cryopreservation, is one of the most proficient methods for ex situ plant germplasm conservation. Whilst seed cryo-banking can have little, to no, or even beneficial effects on subsequent seedling vigor in some species, it can lead to a number of plant abnormalities (morphological and physiological). This study investigated the effects of maize seed cryopreservation on seedling growth (until 14 d) and levels of selected amino acids produced in the shikimate pathway, a major link between primary and secondary metabolism. Seed cryopreservation reduced FW in recovered seedlings, reduced caffeic acid (2.5-fold decrease), and increased levels of all other shikimate pathway–related compounds assessed: phenylalanine (2.9-fold increase), tyrosine (2.6-fold increase), and shikimic (2.1-fold increase) and protocathecuic (3.1-fold increase) acids in cotyledons. Our results suggest that maize seed cryopreservation results in seedlings that exhibit signs of an ‘overly’ efficient and caffeic acid–deficient shikimate pathway, possibly related to their reduced growth during a highly vulnerable growth stage. However, these metabolic abnormalities manifested most severely in the maternal (cotyledonary), as opposed to vegetative (roots, stems, and leaves), tissues and hence are likely to disappear when the seedlings shed the cotyledons and become completely autotrophic.
KeywordsBiochemical Cryopreservation Liquid nitrogen Shikimic acid pathway Phenolics Zea mays L
This research was supported by the Institute of Botany (Leibniz University of Hannover, Germany), the Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López (Ecuador), the University of KwaZulu-Natal (South Africa), and the Bioplant Centre (University of Ciego de Ávila, Cuba). It was also partially supported by the German Academic Exchange Service (DAAD) through a grant to Dr. José Carlos Lorenzo Feijoo. The authors are grateful to Prof. Dr. Jutta Papenbrock, Dr. Yvana Glasenapp, and Dr. Ariel Turcios for their excellent scientific suggestions; and to Mr. José Laguna for his skilled technical assistance.
RP, MA, JM, LH, BEZ, MEMM, LY, S, and JCL designed the research; RP, MA, JM, LH, and JCL conducted the experiments; BEZ, MEMM, LY, S, and JCL wrote the paper; S and JCL had primary responsibility for the final content. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights
This research did not involve experiments with human or animal participants.
Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.
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