In vitro desiccation tolerance of the epiphytic Ghost Orchid, Dendrophylax lindenii (Lindl.) Benth x. Rolfe
The Ghost Orchid, Dendrophylax lindenii (Lindl.) Benth x. Rolfe, is a rare and endangered epiphytic orchid native to south Florida and Cuba. The orchid is considered difficult to propagate under greenhouse conditions, requiring high humidity and low air movement. In contrast, the orchid’s native habitat seasonally dries out with decreased precipitation and humidity. This suggests some level of desiccation tolerance. Ghost Orchid plants were assessed for potential desiccation tolerance and ability to recover from desiccation stress under in vitro conditions. In vitro-derived plants were placed into sterile baby food jars and transferred to chambers maintained at 10% relative humidity, which is extremely low compared to relative humidity levels (40–100%) recorded under natural field conditions. Plants were removed every week for 4 wk and recovered on P723 medium supplemented with banana powder for 4 wk. Data were collected at the initiation of the experiment, after the desiccation periods, and after 4 wk in vitro recovery. Ghost Orchid plants demonstrated extremely high desiccation tolerance. Even after 4 wk desiccation, plant survival was observed at 79.2% after recovery. Desiccated plants exhibited significant decreases in tissue water potential (− 18.44 MPa), fresh weight (65.5% loss), and water content (14.2%); however, high plant survival was still observed under these conditions similar to poikilohydric plants. Overall, the Ghost Orchid demonstrated high desiccation tolerance, which should be considered for future greenhouse culture and for its application in the direct field establishment of in vitro-derived plants without greenhouse acclimatization.
KeywordsDesiccation tolerance Ghost Orchid In vitro Relative humidity
We would like to acknowledge the Gainesville Orchid Society and the Greater Venice Orchid society in Florida, USA, for their financial support to this project. Components of this research were originally supported by funding from the U.S. Fish & Wildlife Service under contract F12AC01245.
- Arditti J (2009) Micropropagation of orchids. In: John Wiley & Sons. Maryland, MaldenGoogle Scholar
- Brown PM (2002) Wild orchids of Florida. University Press of Florida, Gainesville FloridaGoogle Scholar
- Coile NC (2000) Notes on Florida's endangered and threatened plants. In: Botany Contribution No. 38, 3rd edition. Florida Dept. of Agriculture and Consumer Services, Division of Plant Industry, Gainesville FloridaGoogle Scholar
- Davis K (2009) How to grow the ghost orchid: from seedling to flowering. Orchids 78:414–415Google Scholar
- Hoang NH (2016) In vitro physiology of recalcitrant tissue cultured plants in the Nymphaeaceae, Alismataceae, and Orchidaceae. Dissertation. pp 233. University of Florida, Gainesville Florida. Link: http://ufdc.ufl.edu/UFE0049950/00001/citation
- Hoang NH, Kane ME, Radcliffe EN, Zettler LW, Richardson LW (2017) Comparative seed germination and seedling development of the ghost orchid, Dendrophylax lindenii (Orchidaceae), and molecular identification of its mycorrhizal fungus from South Florida. Ann Bot 119:379–393CrossRefPubMedGoogle Scholar
- Orlean S (2000) The orchid thief. Ballantine Books, New York CityGoogle Scholar
- Sony Pictures (2002) Adaptation [Motion Picture]. Kaufman C (Producer), Jonze S (Director) United StatesGoogle Scholar
- Zhang W, Hu H, Zhang SB (2016) Divergent adaptive strategies by two co-occurring epiphytic orchids to water stress: escape or avoidance? Front Plant Sci 7:1–11Google Scholar