Advertisement

Improving micropropagation of Mentha × piperita L. using a liquid culture system

  • B. N. Vaidya
  • B. Asanakunov
  • L. Shahin
  • H. L. Jernigan
  • N. Joshee
  • Sadanand A. DhekneyEmail author
Plant Tissue Culture
  • 65 Downloads

Abstract

In the current study, in vitro shoot proliferation and plant regeneration of Mentha × piperita L. (peppermint) cultivar ‘Black Mitcham’ was compared in semi-solid and liquid culture systems. Shoot tips from field-grown plants were used as explants to study shoot proliferation response on either Murashige and Skoog (MS) or Chee and Pool (C2D) medium containing varying levels of 6-benzylaminopurine (BAP), kinetin, and 6-γ,γ-dimethylallyl aminopurine (2iP). Differences in leaf ultrastructure and antioxidant capacity of greenhouse-grown and micropropagation-derived plants were studied to identify potential changes occurring during in vitro culture. Among the various media treatments tested, the maximum number of shoots was produced on the C2D medium with 4.0 μM BAP (40.7) followed by the MS medium with 4.0 μM BAP (32.2). Among the rooting treatments, shoots on the MS medium with 1.0 μM indole-3-butyric acid (IBA) produced the maximum number of roots (14.4). The number of shoots produced in Liquid Lab Rocker® (LLR) vessels containing liquid C2D medium with BAP (103.4) was significantly higher than that produced on semi-solid medium (40.7). No differences were observed in the leaf ultrastructure and antioxidant capacity of leaf extracts obtained from greenhouse-grown and micropropagation-derived plants. The study indicates that the liquid culture system under the described conditions can enhance peppermint micropropagation, with plant material being potentially valuable for use in herbal supplements and essential oil production.

Keywords

Peppermint Tissue culture Semi-solid medium Liquid medium 

Notes

Acknowledgements

B. Asanakunov was the recipient of the Fulbright Visiting Scientist scholarship. S.A. Dhekney holds the E.A. Whitney Endowed Professorship in the Department of Plant Sciences. H.L. Jernigan was supported in part by a grant from the National Institute of General Medical Sciences (2P20GM103432) from the National Institutes of Health.

References

  1. Adelberg J, Toler J (2004) Comparison of agar and thin-film liquid systems for micropropagation of ornamental Alocasia and Colocasia. HortSci 39:1088–1092Google Scholar
  2. Adelberg J, Cousins MM (2006) Thin films of liquid media for heterotrophic growth and storage organ development: turmeric (Curcuma longa) as a model plant. HortSci 41:539–542Google Scholar
  3. Aflatuni A (2005) The yield and essential oil content of mint (Mentha sp.) in Northern Ostrobothnia. Oulu University, Finland, p 52Google Scholar
  4. Brearley TA, Vaidya BN, Joshee N (2014) Cytokinin, carbon source, and acclimatization requirements for in vitro propagation of Scutellaria barbata D. Don and Scutellaria racemosa Pers. Am J Plant Sci 5:3662–3672CrossRefGoogle Scholar
  5. Buer CS, Imin N, Djordjevic MA (2010) Flavonoids: new roles for old molecules. J Integr Plant Biol 52:98–111CrossRefGoogle Scholar
  6. Cellarova E, Kimakova K, Brutovska R (1992) Multiple shoot formation and phenotypic changes of Ro regenerants in Hypericum perforatum L. Acta Biotechnol 12:445–452CrossRefGoogle Scholar
  7. Chakraborty A, Chattopadhyay S (2008) Stimulation of menthol production in Mentha piperita cell culture. In Vitro Cell Dev Biol - Plant 44:518–524CrossRefGoogle Scholar
  8. Chang C, Ming-Hua Y, Hwe-Mei W, Jiing-Chuang C (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182Google Scholar
  9. Chee R (1986) In vitro culture of Vitis: the effects of light spectrum, manganese sulfate and potassium iodide on morphogenesis. Plant Cell Tissue Organ Cult 7:121–134CrossRefGoogle Scholar
  10. Chee R, Pool RM, Bucher D (1984) A method for large scale in vitro propagation of Vitis. New York’s Food Life Sci Bull 109:1–9Google Scholar
  11. Chee R, Pool RM (1987) Improved inorganic media constituents for in vitro shoot multiplication of Vitis. Sci Hortic 32:85–95CrossRefGoogle Scholar
  12. Combrinck S, Du Plooy GW, McCrindle RI, Botha BM (2007) Morphology and histochemistry of the glandular trichomes of Lippia scaberrima (Verbenaceae). Ann Bot 99:1111–1119CrossRefGoogle Scholar
  13. Croom LA, Jackson CL, Vaidya BN, Joshee N (2016) Thin cell layer (TCL) culture system for herbal biomass production and genetic transformation of Bacopa monnieri L. Wettst. Am J Plant Sci 7:1232–1245CrossRefGoogle Scholar
  14. Dutt M, Li ZT, Dhekney SA, Gray DJ (2007) Transgenic plants from shoot apical meristems of Vitis vinifera L. “Thompson Seedless” via Agrobacterium-mediated transformation. Plant Cell Rep 26:2101–2110CrossRefGoogle Scholar
  15. El-Keltawi NE, Croteau R (1986) Single-node cuttings as a new method of mint propagation. Sci Hortic 29:101–105CrossRefGoogle Scholar
  16. Emershad RL, Ramming DW (1994) Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.). Plant Cell Rep 14:6–12CrossRefGoogle Scholar
  17. Etienne H, Berthouly M (2002) Temporary immersion systems in plant propagation. Plant Cell Tissue Organ Cult 69:215–231CrossRefGoogle Scholar
  18. Faure O, Diemer F, Moja S, Jullien F (1998) Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks. Plant Cell Tissue Organ Cult 52:209–212CrossRefGoogle Scholar
  19. Fortunato M, Avato P, Ruta C (2006) Glandular hairs and essential oils in micropropagated plants of Origanum vulgare L. Acta Hortic 723:295–298Google Scholar
  20. George EF, Hall MA, De Klerk GJ (2008) Plant propagation by tissue culture, vol 1, 3rd edn. Springer, Dordrecht, Netherlands, p 340Google Scholar
  21. Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell Tissue Organ Cult 27:7–14CrossRefGoogle Scholar
  22. Heinrich G, Pfeifhofer HW, Stabentheiner E, Sawidis T (2002) Glandular hairs of Sigesbeckia jorullensis Kunth (Asteraceae): morphology, histochemistry and composition of essential oil. Ann Bot 89:459–469CrossRefGoogle Scholar
  23. Horner CE (1955) Control of peppermint diseases. Station bulletin 547, Agricultural Experiment Station, Oregon State UniversityGoogle Scholar
  24. Hu GX, Balangcod TD, Xiang CL (2012) Trichome micromorphology of the Chinese-Himalayan genus Colquhounia (Lamiaceae), with emphasis on taxonomic implications. Biologia 67:867–874Google Scholar
  25. Huang C, Chen C (2005) Physical properties of culture vessels for plant tissue culture. Biosyst Eng 91:501–511CrossRefGoogle Scholar
  26. Islam MT, Dembele DP, Joachim Keller ER (2005) Influence of explant, temperature and different culture vessels on in vitro culture for germplasm maintenance of four mint accessions. Plant Cell Tissue Organ Cult 81:123–130CrossRefGoogle Scholar
  27. Johnson DA, Baker R, Boydston RA (2013) Field evaluation of mutant and hybrid lines of mint for resistance to Verticillium wilt and yield. Crop Prot 43:1–6CrossRefGoogle Scholar
  28. Kitto S (2016) Micropropagation of mint. In: Beyl CA, Trigiano RN (eds) Plant propagation, concepts and laboratory exercises, second edn. CRC Press, Boca Raton, FL. Chapter 32, pp 385–393Google Scholar
  29. Lee SH, Oh SH, Hwang IG, Kim HY, Woo KS, Woo SH, Kim HS, Lee J, Jeong HS (2016) Antioxidant contents and antioxidant activities of white and colored potatoes (Solanum tuberosum L.). Prev Nutr Food Sci 21:110–116CrossRefGoogle Scholar
  30. Li X, Niu X, Bressan RA, Weller SC, Hasegawa PM (1999) Efficient plant regeneration of native spearmint (Mentha spicata L.). In Vitro Cell Dev Biol - Plant 35:333–338CrossRefGoogle Scholar
  31. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  32. Magdalita PM, Godwin ID, Drew RA, Adkins SW (1997) Effect of ethylene and culture environment on development of papaya nodal cultures. Plant Cell Tissue Organ Cult 49:93–100CrossRefGoogle Scholar
  33. McKay DL, Blumberg JB (2006) A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytother Res 20:619–633CrossRefGoogle Scholar
  34. Mphahlele RR, Fawole OA, Makunga NP, Opara U (2016) Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activity of pomegranate peel. BMC Complement Altern Med 16:143CrossRefGoogle Scholar
  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  36. Passinho-Soares HC, David JP, de Santana JRF, David JM, Rodrigues FM, Mesquita PRR, de Oliveria FS, Bellintani MC (2017) Influence of growth regulators on distribution of trichomes and the production of volatiles in micropropagated plants of Plectranthus ornatus. Rev Bras 27:679–690Google Scholar
  37. Philips GC (2004) In vitro morphogenesis in plants—recent advances. In Vitro Cell Dev Biol-Plant 40:342–345CrossRefGoogle Scholar
  38. Peer WA, Brown DE, Tague BW, Muday GK, Taiz L, Murphy AS (2001) Flavonoid accumulation patterns of transparent testa mutants of Arabidopsis. Plant Physiol 126:536–548CrossRefGoogle Scholar
  39. Re R, Pellegrini N, Proteggente A, Pannala A, Yang C, Rice-Evans M (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26:1231–1237CrossRefGoogle Scholar
  40. Reed BM (1999) In vitro storage conditions for mint germplasm. HortSci 34:350–352Google Scholar
  41. Roshanak S, Rahimmalek M, Goli SAH (2016) Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. assamica) leaves. J Food Sci Technol 53:721–729CrossRefGoogle Scholar
  42. Seigler DS (1998) Shikimic acid pathway. In: Siegler DS (ed) Plant secondary metabolism. Springer, Boston, MA. Chapter 8, pp 94–105.  https://doi.org/10.1007/978-1-4615-4913-0 CrossRefGoogle Scholar
  43. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotunstic acid reagents. Am J Enol Vitic 16:144–158Google Scholar
  44. Tascan A, Adelberg JW, Rimando AM, Tascan M, Joshee N, Yadav AK (2010) Hyperhydricity and flavonoid content of Scutellaria species in vitro on polyester-supported liquid culture systems. HortSci 45:1723–1728Google Scholar
  45. Tisserat B, Silman R (2000) Interactions of culture vessels, media volume, culture density, and carbon dioxide level on lettuce and spearmint shoot growth in vitro. Plant Cell Rep 19:464–471CrossRefGoogle Scholar
  46. Tisserat B, Vaughn S (2008) Growth, morphogenesis and essential oil production in Mentha spicata L. shoots in vitro. In Vitro Cell Dev Biol-Plant 44:40–50CrossRefGoogle Scholar
  47. Vaidya BN, Brearley TA, Joshee N (2013) Antioxidant capacity of fresh and dry leaf extracts of sixteen Scutellaria species. J Med Active Plant 2:42–49Google Scholar
  48. Van Eck JM, Kitto SL (1992) Regeneration of peppermint and orange mint from leaf disks. Plant Cell Tissue Organ Cult 30:41–49CrossRefGoogle Scholar
  49. Veronese P, Li X, Niu X, Weller SC, Bressan RA, Hasegawa PM (2001) Bioengineering mint crop improvement. Plant Cell Tissue Organ Cult 64:133–144CrossRefGoogle Scholar
  50. Wagner H, Bladt S (1996) Plant drug analysis: a thin layer chromatography atlas, 2nd edn. Springer-Verlag, Berlin.  https://doi.org/10.1007/978-3-642-00574-9 CrossRefGoogle Scholar
  51. Wang PJ, Charles A (1991) Micropropagation through meristem culture. In: Bajaj YPS (ed) High-tech and micropropagation I. Biotechin Agr and Forestry, vol 17. Springer, Berlin, HeidelbergGoogle Scholar
  52. Wang X, Gao Z, Wang Y, Bressan RA, Weller SC, Li X (2009) Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants. In Vitro Cell Dev Biol - Plant 45:435–440CrossRefGoogle Scholar
  53. Yi W, Wetzstein H (2010) Biochemical, biological and histological evaluation of some culinary and medicinal herbs grown under greenhouse and field conditions. J Sci Food Agric 90:1063–1070Google Scholar
  54. Zhao Y, Zhou Y, Grout BWW (2006) Variation in leaf structures of micropropagated rhubarb. Plant Cell Tissue Organ Cult 85:115–121CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  • B. N. Vaidya
    • 1
  • B. Asanakunov
    • 2
  • L. Shahin
    • 1
  • H. L. Jernigan
    • 2
  • N. Joshee
    • 1
  • Sadanand A. Dhekney
    • 2
    Email author
  1. 1.Graduate Program in BiotechnologyFort Valley State UniversityFort ValleyUSA
  2. 2.Sheridan Research and Extension CenterUniversity of WyomingSheridanUSA

Personalised recommendations