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Acid pretreatment improves microtuberization of potato plantlets

  • Yue Teng
  • Yan Zhang
  • Jin Ting Guo
  • Yu Liang Gao
  • Kui Hua LiEmail author
Micropropagation
  • 24 Downloads

Abstract

To efficiently produce potato (Solanum tuberosum L.) microtubers, in vitro plantlets were pretreated with an acid solution before culturing in Murashige and Skoog medium supplemented with 60 g L−1 sucrose. The first tubers were formed at 36 h of acid pretreatment (AT), which was 6.2 d earlier than the untreated control. The maximum number of tubers (3.2), and highest fresh weight per tuber (282.5 mg) were also achieved with the AT. During microtuberization, the leaf dry weight of the control was higher than the AT from 1 to 5 d of culture. However, the dry weight of AT leaves increased rapidly after 5 d of culture and reached the same level as the control. The tuber dry weight in the AT was significantly higher (α = 0.05) than the control during the same culture period. Sucrose and starch content, and the enzyme activity of sucrose synthase (SS), ADP-glucose pyrophosphorylase (AGPase), and soluble starch synthase (SSS) in the AT and control exhibited a similar pattern as culture time progressed. However, levels of these biochemical indexes statistically differed (α = 0.05) between the AT and control. In addition to the sucrose content in AT, the starch content and activities of SS, AGPase, and SSS in the leaves were lower, whereas those in tubers were higher than those in the control. The present study suggests that acid pretreatment can efficiently promote microtuber formation and growth, which can be used for industrial production of potato seeds.

Keywords

Acid stress Acid treatment time Microtuber Carbohydrate metabolism 

Notes

Funding information

Project 31260470 is supported by the National Natural Science Foundation of China.

References

  1. Aksenova NP, Konstantinova TN, Golyanovskaya SA, Sergeeva LI, Romanov GA (2012) Hormonal regulation of tuber formation in potato plants. Russ J Plant Physiol l59:451–466CrossRefGoogle Scholar
  2. Arnd S, Tang GQ (1999) The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci 99:1360–1385Google Scholar
  3. Bánfalv Z, Molnár A, Lakatos L, Hesse H, Höfgen R (1999) Differences in sucrose-to-starch metabolism of Solanum tuberosum and Solanum brevidens. Plant Sci 147:81–88CrossRefGoogle Scholar
  4. Bánfalv Z, Molnfir A, Molnfir G, Lakatos L, Szabo L (1996) Starch synthesis-, and tuber storage protein genes are differently expressed in Solanum tuberosum and in Solanum brevidens. FEBS Lett 383:159–164CrossRefGoogle Scholar
  5. Barnard R, Combrink NJJ (2004) Potato mini tuber production affected by a short-term calcium deficiency. S Afr J Plant Soil 21:200–202CrossRefGoogle Scholar
  6. Baroja-Fernández E, Muñoz FJ, Montero M, Etxeberria E, Sesma MT, Ovecka M, Bahaji A, Ezquer I, Li J, Prat S, Pozueta-Romero J (2009) Enhancing sucrose synthase activity in transgenic potato (Solanum tuberosum L.) tubers results in increased levels of starch, ADPglucose and UDPglucose and total yield. Plant Cell Physiol 50:1651–1662CrossRefGoogle Scholar
  7. Cao W, Tibbitts TW (1997) Starch concentration and impact on specific leaf weight and element concentrations in potato leaves under varied carbon dioxide and temperature. J Plant Nutr 20:871–881CrossRefGoogle Scholar
  8. Dong CC, Park CS, Lee JG, Lee JH, Son JM, Lee YB (2005) Optimizing electrical conductivity and pH of nutrient solution for hydroponic culture of seed potatoes (Solanum tuberosum). Hortic Environ Biotechnol 46:26–32Google Scholar
  9. Donnelly DJ, Coleman WK, Coleman SE (2003) Potato microtuber production and performance: a review. Amer J Potato Res 80:103–115CrossRefGoogle Scholar
  10. Edwards A, Fulton DC, Hylton CM, Jobling SA, Gidley M, Martin C, Smith AM (1999) A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers. Plant J 17:251–261CrossRefGoogle Scholar
  11. Emes MJ, Bowsher CG, Hedley C, Burrell MM, Scrase-Field ESF, Tetlow IJ (2003) Starch synthesis and carbon partitioning in developing endosperm. J Exp Bot 54:569–575CrossRefGoogle Scholar
  12. Geigenberger P, Reimholz R, Deiting U, Sonnewald U, Stitt M (1999) Decreased expression of sucrose phosphate synthase strongly inhibits the water stress-induced synthesis of sucrose in growing potato tubers. Plant J 19:119–129CrossRefGoogle Scholar
  13. Geigenberger P, Stitt M, Fernie A (2004) Metabolic control analysis and regulation of the conversion sucrose to starch. Plant Cell Environ 27:655–673CrossRefGoogle Scholar
  14. Gopal J, Minocha JL, Dhaliwal HS (1998) Microtuberization in potato (Solanum-tuberosum L.). Plant Cell Rep 17:794–798CrossRefGoogle Scholar
  15. Gudeva LK, Trajkova F, Stojkova I (2016) The effect of plant growth regulators and sucrose on microtuberization of potato (Solanum tuberosum L.). Rom Agr Res 33:1–7Google Scholar
  16. Halford N, Paul MJ (2003) Carbon metabolite sensing and signalling. Plant Biotechnol J 1:381–389CrossRefGoogle Scholar
  17. Hendriks JHM, Kolbe A, Gibon Y, Stitt M, Geigenberger P (2003) ADP-glucose pyrophosphorylase is activated by posttranslational redox-modification in response to light and to sugars in leaves of Arabidopsis and other plant species. Plant Physiol 133:838–849CrossRefGoogle Scholar
  18. Hoque ME (2010) In vitro tuberization in potato ( Solanum tuberosum L.). Plant Omics J 3:7–11Google Scholar
  19. Jeon JS, Ryoo N, Hahn TR, Walia H, Nakamura Y (2010) Starch biosynthesis in cereal endosperm. Plant Physiol Biochem 48:383–392CrossRefGoogle Scholar
  20. Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235–246CrossRefGoogle Scholar
  21. Lian ML, Piao XC, Park SY (2014) Mass production of Lilium bublets in bioreactors. In: Paek KY, Murthy H, Zhong JJ (eds) Production of biomass and bioactive compounds using bioreactor technology. Dordrecht Heidelberg New York. Springer, LondonGoogle Scholar
  22. Lipiec J, Doussan C, Nosalewicz A, Kondracka K (2013) Effect of drought and heat stresses on plant growth and yield: a review. Int Agrophys 27:463–477CrossRefGoogle Scholar
  23. Lu GQ, Li XL, Ding SR (2002) Quick analysis of starch content of sweet potato by HCl hydrolysis-DNS method. J Chin Cereals Oils Assoc 17:25–28Google Scholar
  24. MacGregor DR, Deak KI, Ingram PA, Malamy JE (2008) Roots system architecture in Arabidopsis grown in culture is regulated by sucrose uptake in the aerial tissues. Plant Cell 20:2643–2660CrossRefGoogle Scholar
  25. Miao YY, Zhu ZB, Guo QS, Yang XH, Liu L, Sun Y, Wang CL (2016) Dynamic changes in carbohydrate metabolism and endogenous hormones during Tulipa edulis stolon development into a new bulb. J Plant Biol 59:121–132CrossRefGoogle Scholar
  26. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  27. Niek JGA, Steef MB, Elly AMK, Richard GFV, Dick V, Linus HWP (1997) Developmental changes of enzymes involved in conversion of sucrose to hexose-phosphate during early tuberisation of potato. Planta 202:220–226CrossRefGoogle Scholar
  28. Oraby H, Lachance A, Desjardins Y (2015) A low nutrient solution temperature and the application of stress treatments increase potato mini-tubers production in an aeroponic system. Am J Potato Res 92:387–397CrossRefGoogle Scholar
  29. Piao XC, Chakrabarty D, Hahn EY, Paek KY (2004) The growth and photosynthetic characteristics of potato (Solanum tuberosum L.) plantlets as affected by hydroponic solution pH and EC, light, and CO2. J Am Soc Hortic Sci 129:100–105Google Scholar
  30. Puijalon S, Piola F, Bornette G (2007) Abiotic stresses increase plant regeneration ability. Evol Ecol 22:493–506CrossRefGoogle Scholar
  31. Pumisutapon P, Topoonyanont N (2017) Moderate-abiotic stress increase in vitro tuberization and microtuber growth of potato. Acta Hortic 1155:215–220CrossRefGoogle Scholar
  32. Pumisutapon P, Visser RGF, Klerk GJD (2012) Moderate abiotic stresses increase rhizome growth and outgrowth of axillary buds in Alstroemeria cultured in vitro. Plant Cell Tissue Organ Cult 110:395–400CrossRefGoogle Scholar
  33. Raíces M, Ulloa RM, MacIntosh GC, Crespi M, Téllez-iñón M (2003) StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration. J Exp Bot 54:2589–2591CrossRefGoogle Scholar
  34. Ranalli P, Bassi F, Ruaro G, Delre P, Dicandilo M, Mandolino G (1994) Microtuber and minituber production performance compared with normal tubers and field. Potato Res 37:383–391CrossRefGoogle Scholar
  35. Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants conserved and novel mechanisms. Plant Biol 57:675–709CrossRefGoogle Scholar
  36. Ross HA, Davies HV (1992) Sucrose metabolism in tubers of potato (Solanum tuberosum L.): effects of sink removal and sucrose flux on sucrose-degrading enzymes. Plant Physiol 98:287–293CrossRefGoogle Scholar
  37. Ruan YL, Jin Y, Yang YJ, Li GJ, Boyer JS (2010) Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Mol Plant 3:942–955CrossRefGoogle Scholar
  38. Sweetlove LJ, Burrell MM, Rees T (1996) Starch metabolism in tubers of transgenic potato (Solanum tuberosum) with increased ADPglucose pyrophosphorylase. Biochem J 320:493–498CrossRefGoogle Scholar
  39. Teng Y, Gao YL, Zhang Y, Jin MY, Li KH (2017) Effects of acid stress on microtuberization and some glycometabolism of potato in vitro plantlets. Crops 33:140–143Google Scholar
  40. Thorpe T, Meier D (1972) Starch metabolism, respiration, and shoot formation in tobacco callus cultures. Physiol Plant 27:365–369CrossRefGoogle Scholar
  41. Wan WY, Cao W, Tibbitts TW (1994) Tuber initiation in hydroponically grown potatoes by alteration of solution pH. Hortic Sci 29:261–263Google Scholar
  42. Wang BS, Ma MY, Lu HG, Meng QW, Li G, Yang XH (2015) Photosynthesis, sucrose metabolism, and starch accumulation in two NILs of winter wheat. Photosynth Res 126:363–373CrossRefGoogle Scholar
  43. Xu WF, Jia LG, Shi WM, Liang JS, Zhou F, Li QF, Zhang JH (2013) Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress. New Phytol 197:139–150CrossRefGoogle Scholar
  44. Zhang ZJ, Mao BZ, Li HZ, Zhou WJ, Takeuchi Y, Yoneyama K (2005) Effect of salinity on physiological characteristics, yield and quality of microtubers in vitro in potato. Acta Physiol Plant 27:481–489CrossRefGoogle Scholar

Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  • Yue Teng
    • 1
  • Yan Zhang
    • 2
  • Jin Ting Guo
    • 1
  • Yu Liang Gao
    • 3
  • Kui Hua Li
    • 1
    Email author
  1. 1.Agricultural CollegeYanbian UniversityJilinChina
  2. 2.Jilin City Academy of Agricultural SciencesJilinChina
  3. 3.Yanbian Agricultural Sciences AcademyJilinChina

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