Acid pretreatment improves microtuberization of potato plantlets
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To efficiently produce potato (Solanum tuberosum L.) microtubers, in vitro plantlets were pretreated with an acid solution before culturing in Murashige and Skoog medium supplemented with 60 g L−1 sucrose. The first tubers were formed at 36 h of acid pretreatment (AT), which was 6.2 d earlier than the untreated control. The maximum number of tubers (3.2), and highest fresh weight per tuber (282.5 mg) were also achieved with the AT. During microtuberization, the leaf dry weight of the control was higher than the AT from 1 to 5 d of culture. However, the dry weight of AT leaves increased rapidly after 5 d of culture and reached the same level as the control. The tuber dry weight in the AT was significantly higher (α = 0.05) than the control during the same culture period. Sucrose and starch content, and the enzyme activity of sucrose synthase (SS), ADP-glucose pyrophosphorylase (AGPase), and soluble starch synthase (SSS) in the AT and control exhibited a similar pattern as culture time progressed. However, levels of these biochemical indexes statistically differed (α = 0.05) between the AT and control. In addition to the sucrose content in AT, the starch content and activities of SS, AGPase, and SSS in the leaves were lower, whereas those in tubers were higher than those in the control. The present study suggests that acid pretreatment can efficiently promote microtuber formation and growth, which can be used for industrial production of potato seeds.
KeywordsAcid stress Acid treatment time Microtuber Carbohydrate metabolism
Project 31260470 is supported by the National Natural Science Foundation of China.
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