miR-140-3p exhibits repressive functions on preosteoblast viability and differentiation by downregulating MCF2L in osteoporosis

  • Jin-He Mao
  • Yu-Xin Sui
  • Shuang Ao
  • Yu Wang
  • Yu Liu
  • Hui LengEmail author


Previous research manifested that miR-140-3p was a latent biomarker for osteoporosis. Nevertheless, the mechanism of miR-140-3p in osteoporosis is still not clear and needs ulteriorly studying. The purpose of our paper was to ulteriorly probe the underlying mechanism of miR-140-3p on osteoporosis. Firstly, based on the data acquired from GEO database, we found that miR-140-3p was highly expressed; meanwhile, MCF2L was lowly expressed in osteoporosis patients. Upregulation/downregulation of miR-140-3p by miR-140-3p mimic/inhibitor restrained/promoted MC3T3-E1 cell viability and differentiation. However, miR-140-3p over-expression/downregulation accelerated/repressed MC3T3-E1 cell apoptosis. MCF2L was forecasted as a target of miR-140-3p by miRanda, miRWalk, and TargetScan miRNA target gene prediction software. Luciferase reporter assay confirmed that MCF2L could be directly targeted by miR-140-3p. Moreover, we identified that the expression of MCF2L was negatively regulated by miR-140-3p. From rescue assays, we discovered that knockdown of MCF2L weakened the promoting influence of miR-140-3p ablation on MC3T3-E1 cell viability and differentiation, and receded the suppressing impact of miR-140-3p reduction on MC3T3-E1 cell apoptosis. Above all, this research disclosed that miR-140-3p repressed preosteoblast viability and differentiation while promoted preosteoblast apoptosis via targeting MCF2L. Our discoveries might afford a theoretical basis of developing a latent novel target for osteoporosis therapy.


miR-140-3p MCF2L Osteoporosis Viability Differentiation Apoptosis 


Authors’ contributions

Jin-He Mao and Yu-Xin Sui performed the experiment. Shuang Ao and Yu Wang analyzed and interpreted the data. Yu Liu drafted the article. Hui Leng designed the study, and critically revised the manuscript. All authors have read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  • Jin-He Mao
    • 1
  • Yu-Xin Sui
    • 1
  • Shuang Ao
    • 1
  • Yu Wang
    • 1
  • Yu Liu
    • 1
  • Hui Leng
    • 1
    Email author
  1. 1.Department of OrthopedicsChifeng Municipal HospitalChifengPeople’s Republic of China

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