SNHG14 promotes the tumorigenesis and metastasis of colorectal cancer through miR-32-5p/SKIL axis

  • Tao Ye
  • Ning Zhang
  • Wenyu Wu
  • Bing Yang
  • Jinghui Wang
  • Wenqi Huang
  • Dongxin TangEmail author


Colorectal cancer (CRC) is regarded as one of the top ten malignant cancers, which has caused millions of mortalities all over the world. Although advanced therapeutic methods have been employed to treat CRC, the prognosis of CRC patients remains unsatisfactory. Many researchers claimed long noncoding RNAs (lncRNAs) frequently participate in the development of cancers. Small nucleolar RNA host gene 14 (SNHG14) was proved to play roles in various cancers. Nevertheless, neither biological function nor regulatory mechanism of SNHG14 has been explored in CRC. This investigation is aimed at exploring the role of SNHG14 in CRC. The expression of genes including SNHG14, miR-32-5p, and ski-oncogene-like (SKIL) was measured by RT-qPCR assay. 5-Ethynyl-2′-deoxyuridine (EdU) assay was employed to measure cell proliferation. Cell migration and invasion were evaluated by transwell assay. Western blot assay was performed to test the protein expression. The binding capacity between miR-32-5p and SNHG14 (or SKIL) was explored by luciferase reporter and RNA immunoprecipitation (RIP) assays. SNHG14 expression is upregulated in CRC cells. Moreover, SNHG14 suppression inhibited the proliferation, metastasis, and epithelial-mesenchymal transition (EMT) process in CRC cells. miR-32-5p presented lower expression, which was negatively regulated by SNHG14. SKIL could combine with miR-32-5p. The mRNA and protein expression of SKIL was downregulated by SNHG14 knockdown or miR-32-5p overexpression. At last, the inhibitory effect of SNHG14 suppression on proliferation, metastasis, and EMT process was rescued by SKIL overexpression. SNHG14 regulates CRC progression via miR-32-5p/SKIL axis, providing a novel point in treatment of CRC patients.


SNHG14 SKIL miR-32-5p CRC 



We appreciate the support of Regional Science Fund Project of the National Natural Science Foundation of China (Nos. 81760814, 81860819), High-level Innovative Talent Training Plan of Guizhou (100 levels) (the Qian family matches the talented person [2016] No. 4032), TCM Tumor Inheritance and Scientific And Technological Innovation Talent Base of Guizhou Province (Guizhou people lead [2018] No. 3), Doctor Foundation of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine (GYZYYFY-BS-2018[10]) and Research Project of Traditional Chinese Medicine and Ethnic Medicine Science and Technology of Guizhou Provincial Administration of Traditional Chinese Medicine (No. QZYY-2019-18).

Compliance with ethical standards

The present investigation was approved by the committee of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine

Conflict of interest

The authors declare that there are no competing interests.

Supplementary material

11626_2019_398_Fig6_ESM.png (297 kb)
Fig S1

(A-B) The expression of proteins related to TGF-β and Wnt/β-catenin signaling pathway was examined by western blot assay. (C) The expression of SNHG14 was tested by RT-qPCR. *P < 0.05, **P < 0.01. (PNG 297 kb)

11626_2019_398_MOESM1_ESM.tif (13.6 mb)
High Resolution Image (TIF 13877 kb)


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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Oncology DepartmentThe First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangChina
  2. 2.Pharmacy DepartmentThe First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangChina
  3. 3.Research LaboratoryThe First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangChina
  4. 4.Department of Science and EducationThe First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangChina

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