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Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

  • Tamra Reall
  • Susanne Kraus
  • Cynthia L. GoodmanEmail author
  • Joseph RingbauerJr
  • Sven Geibel
  • David Stanley
Article

Abstract

The fall armyworm, Spodoptera frugiperda (Sf), is a polyphagous lepidopteran herbivore that consumes more than 80 plant species, including many economically important crops, such as corn, soybeans, and sorghum. While already a serious pest in the Americas, it was recently introduced into Africa, India, and China. Because of its high economic costs in the New World and the continent-wide damage potentials in Africa, research to develop advanced pest management technologies is necessary. We are supporting this need by developing novel, next-generation insect cell lines from targeted tissues. Cell lines, such as these, will boost insecticide discovery programs and lead to innovative pest management solutions. Here, we report on the establishment of 16 new cell lines from larval S. frugiperda tissues: nine from the central nervous system, three from the aorta, and four from the testes. We confirmed the identities of the cell lines by DNA amplification fingerprinting polymerase chain reaction, determined their doubling times from growth curves, and described cell types via microscopy. We also developed 16 sublines from three neuronal cell lines.

Keywords

Insect cell line Lepidoptera Fall armyworm Spodoptera frugiperda DAF-PCR Agriculture 

Notes

Acknowledgments

Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the USDA are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. We acknowledge the support of BAYER AG through Cooperative Research Agreement 58-3K95-5-1728.

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Biological Control of Insects Research LaboratoryUSDA/Agricultural Research ServiceColumbiaUSA
  2. 2.Bayer AGMonheim am RheinGermany

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