Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro
Androgens are involved in maintaining epididymal structure and function. In the present study, primary culture of goat EECs and effect of testosterone on expression of glutathione peroxidase-5 (GPX5) in goat epididymal epithelial cells (EECs) were investigated. The EECs isolated from 12-mo-old goat caput epididymis were cultured with testosterone in vitro, and expression of glutathione peroxidase-5 (GPX5) and androgen receptors (ARs) was analyzed. Our results showed that testosterone effectively increased EEC proliferation activity, and EECs cultured with testosterone could maintain molecular markers for up to 12 passages. Compared with the control group, 100 nM testosterone significantly increased the mRNA and protein expression of GPX5 (P < 0.05) and ARs (P < 0.01 and P < 0.05, respectively) in EECs, and this effect was blocked by the AR blocker enzalutamide. In conclusion, testosterone can promote the expression of GPX5 in EECs by up-regulating AR expression. We established an effective culture system for goat EECs which can be for further investigation on the regulation of epithelial function.
KeywordsEpididymal epithelial cells Primary culture Testosterone GPX5 Androgen receptors
Z.L. designed the research, conducted experiments, and wrote the manuscript. X.F. performed the experiments and prepared the figures. H.S. and R.L. contributed to data acquisition. W.Z. and J.Z supervised the project and wrote the paper.
This work was supported by the Natural Science Foundation of Inner Mongolia Autonomous Region of China (No. 2018MS03012).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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