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LncRNA KCNQ1OT1 acting as a ceRNA for miR-4458 enhances osteosarcoma progression by regulating CCND2 expression

  • Meng WangEmail author
  • Zengtao Wang
  • Xiaolei Zhu
  • Shibing Guan
  • Zhibo LiuEmail author
Article
  • 82 Downloads

Abstract

Osteosarcoma is prevalent worldwide and characterized as a challenging health burden. It has been increasingly indicated that long non-coding RNAs (lncRNAs) are significant in pathological processes of numerous cancers, exerting oncogenic or tumor-suppressive function. However, the participation of KCNQ1OT1 in osteosarcoma has not been elaborated. In this study, we focus on interrogating the function of KCNQ1OT1 and its underlying mechanism in osteosarcoma. Our work demonstrated the upregulation of KCNQ1OT1 in osteosarcoma through qRT-PCR. Besides, loss of function assay (CCK-8, transwell migration) indicated KCNQ1OT1 promoted cell proliferation, migration in osteosarcoma. Mechanically, KCNQ1OT1 acting as sponge for miR-4458 antagonized its tumor-suppressive impact on CCND2 expression. The anti-apoptotic nature of KCNQ1OT1 was also unveiled via caspase-3 activity assay. Overexpressed KCNQ1OT1 acted as competing endogenous RNA (ceRNA) for miR-4458 and subsequently reinforced target gene CCND2. Collectively, the results of rescue experiments suggested that the oncogenic role of KCNQ1OT1 was performed through sponging miR-4458 and upregulating CCND2 during osteosarcoma development, providing a novel perspective of intervention in osteosarcoma management.

Keywords

KCNQ1OT1 miR-4458 CCND2 Osteosarcoma 

Notes

Acknowledgments

We are thankful to all who participated in this study.

Compliance with ethical standards

The Research Ethics Committee of this hospital gave consent to this study. Prior to diagnosis, none of patients were subjected to antitumor treatment. The written consents were eligibly gained from OS patients and in line with the Declaration of Helsinki, the study was implemented.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11626_2019_386_Fig5_ESM.png (216 kb)
Figure S1

(A) KCNQ1OT1 and CCND2 levels in OS tissues and non-OS tissues were examined via qRT-PCR analysis. (PNG 216 kb)

11626_2019_386_MOESM1_ESM.tif (6.4 mb)
High resolution image (TIF 6551 kb)

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Hand and Foot Surgical CenterProvincial Hospital Affiliated to Shandong UniversityJinanChina

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