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Targeted induction of bone marrow mesenchymal stem cells to have effectiveness on diabetic pancreatic restoration

  • Jing Zhang
  • Ruonan Mao
  • Xinyu Wang
  • Kun Liu
  • Qi Geng
  • Yijin Yu
  • Yanning LiEmail author
  • Jinsheng QiEmail author
Article
  • 32 Downloads

Abstract

Although bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to be effective for the attenuation of diabetes, they have limitations. Whether BMSCs can be target-induced by pancreatic stem cells (PSCs) to have effectiveness for the restoration of diabetic islet injury was unknown. In this study, based on their successful isolation and cultivation, BMSCs were co-cultured with PSCs. The pancreatic stem cells markers, Nestin and Neurogenin3 in co-cultured BMSCs were detected to evaluate the target-induction effects. After the diabetic rats were intravenously injected with the target-induced BMSCs, general indicators and islet morphology were detected. The islet insulin generation, and serum insulin and C-peptide contents were measured. It was found that after co-culture, the mRNA expressions, protein contents and distributions of Nestin and Neurogenin3, were dramatically high in BMSCs, indicating that they were successfully target-induced to pancreatic stem-like cells. Furthermore, the target-induced BMSCs had beneficial effects on serum glycated albumin levels and glycogen contents as well as islet morphology of the diabetic rats. Besides elevation of islet insulin generation, the target-induced BMSCs had significant effect on serum insulin and C-peptide contents. In conclusion, BMSCs could be target-induced by PSCs to have effectiveness on the pancreatic restoration of diabetic rats.

Keywords

Bone marrow mesenchymal stem cells Pancreatic stem cells Targeted induction Pancreatic restoration 

Notes

Financial funding

This work was supported by Grants from the National Natural Science Foundation of China (81470595 and 81600384) and the Hebei Natural Science Foundation (H2015206101).

Compliance with ethical standards

Experiment protocol followed the guidelines established by the Ethics Review Committee for Animal Experimentation of Hebei Medical University.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

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Copyright information

© The Society for In Vitro Biology 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, Hebei Key Laboratory of Medical BiotechnologyHebei Medical UniversityShijiazhuangPeople’s Republic of China
  2. 2.Department of Molecular Biology, Hebei Key Lab of Laboratory Animal ScienceHebei Medical UniversityShijiazhuangPeople’s Republic of China

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