Antitumor effects of SB injection in canine osteosarcoma and melanoma cell lines

  • Kyeong Kweon
  • Jin-Ok Ahn
  • Woo-Jin Song
  • Qiang Li
  • Bo-Yeon Lee
  • Hyung-Kyu Chae
  • Hwa-Young YounEmail author


The present study was designed to evaluate the effect of SB injection, which is composed of extracts from the roots of Pulsatilla koreana, Panax ginseng, and Glycyrrhiza glabra, on the viability of canine osteosarcoma and melanoma cells and nonneoplastic canine cells. Cells were treated with SB injection, conventional chemotherapeutic drugs, or a combination of both at various concentrations. Cellular viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry was used to evaluate the cell cycle and apoptosis. SB injection inhibited the growth of osteosarcoma and melanoma cells in a dose-dependent manner. The cell cycle of the affected cells was arrested in the G2/M phase, indicating an anti-proliferative effect. SB injection dose-dependently increased the rate of apoptosis. Furthermore, we found that combining SB injection with chemotherapeutic drugs resulted in a greater reduction in canine malignant cell proliferation than either treatment alone. SB injection did not affect the viability of peripheral blood mononuclear cells regardless of concentration, which suggested that SB injection did not suppress the activity of normal cells. This study suggested that SB injection can be considered an effective alternative medication for animal cancers in veterinary medicine.


Natural extract Antitumor agent Canine osteosarcoma Canine melanoma 



This study was supported by the Research Institute for Veterinary Science and BK21 PLUS Program for Creative Veterinary Science Research.

Compliance with ethical standards

Conflict of interest statement

There are no conflicts of interest to declare.


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Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  1. 1.Department of Veterinary Internal Medicine, College of Veterinary MedicineSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Veterinary Internal Medicine, College of Veterinary MedicineKangwon National UniversityKangwondoRepublic of Korea
  3. 3.Department of Veterinary Internal Medicine and Research Institute for Veterinary Science, College of Veterinary MedicineSeoul National UniversitySeoulRepublic of Korea

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