Isolation and identification of bovine nasopharyngeal mucosal epithelial cells and establishment of cell models of acute infection by foot-and-mouth disease virus

  • Wan-fu Bai
  • Lu Li
  • Ting Zhang
  • Xiao-hu Su
  • Yong-wei Wang
  • Bing-wu Zhao
  • Tao Zhang
  • Huan-min Zhou
Article
  • 98 Downloads

Abstract

Foot-and-mouth disease (FMD) commonly occurs via the respiratory tract, and bovine nasopharyngeal mucosal epithelial cells are the primary infection cells in cattle. The aim of the present study was to isolate and culture epithelial cells from the bovine nasopharyngeal mucosa in vitro using a mechanical separation method. The cells were expanded, established in continuous cell culture, and used for immunofluorescence cytochemistry and establishment of infection models. We detected pan-cytokeratin markers of bovine nasopharyngeal mucosal epithelial cells by immunofluorescence. Bovine nasopharyngeal mucosal epithelial cells were then infected with foot-and-mouth disease virus (FMDV) serum type O. RT-PCR demonstrated the successful establishment of acute FMDV infection in the cell models. This infection model provides the basis for clarification of the interaction between FMDV and host bovine nasopharyngeal mucosal epithelial cells in vitro.

Keywords

Bovine nasopharyngeal mucosal epithelial cells Cell isolation Cell identification Infection models FMDV 

Notes

Acknowledgements

We sincerely acknowledge the help of all the members of Research and Development Department of Inner Mongolia Bigvet Biotech Co., Ltd. We thank Dr. Eerdunmutu (Inner Mongolia Agricultural University) for sample collection and discussions.

Compliance with ethical standards

Animals were sacrificed in accordance with the guidelines of the Ethics Committee of the Institute of Inner Mongolia Agricultural University.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11626_2018_235_MOESM1_ESM.docx (15 kb)
ESM 1 (DOCX 14 kb)

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Copyright information

© The Society for In Vitro Biology 2018

Authors and Affiliations

  1. 1.Inner Mongolia Agricultural UniversityHohhotPeople’s Republic of China
  2. 2.Baotou medical collegeInner Mongolia University of Science & TechnologyBaotouPeople’s Republic of China
  3. 3.Inner Mongolia biological manufacturing key laboratoryHohhotPeople’s Republic of China
  4. 4.Sun Yat-sen UniversityGuangzhouPeople’s Republic of China
  5. 5.Inner Mongolia Bigvet Biotech Co., LtdHohhotPeople’s Republic of China

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