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A Highly Elastic and Adhesive Gelatin Tissue Sealant for Gastrointestinal Surgery and Colon Anastomosis

Abstract

Background

We describe the development of a highly elastic and adhesive surgical tissue sealant, based on photochemically crosslinked gelatin, for sealing sutured incisions in the gastrointestinal (GI) tract in a rabbit surgical model and in a canine colon anastomosis study.

Methods

The study included in vitro assessment of mechanical parameters of the tissue sealant and in vivo analysis of burst strength and histology at 24 h, 3 days and 7 days post surgery, in a rabbit model, to assess progress of wound healing at the suture sites. Utility of this sealant to repair and seal a lower colonic resection and anastomosis procedure in a canine model was also investigated.

Results

We show that a photopolymerised gelatin tissue sealant provides effective sealing of GI incisions and facilitates wound healing with no evidence of inflammation up to 28 days post-surgery. Blending of derivatised gelatin with underivatised gelatin allowed tuning of elasticity and elastic modulus of the photopolymerised sealant to suit surgical applications. High tissue adhesive strength was maintained at all blend ratios and exceeded 100 kPa.

Conclusions

This highly elastic and adhesive photopolymerised gelatin tissue sealant offers a number of advantages over currently available sealants suitable for GI surgical procedures.

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Acknowledgements

We wish to thank Kathleen Kimpton, Tara Gallagher, Jason Grimmer (CSIRO Food and Nutritional Sciences) for assistance with animal surgery.

Author information

Correspondence to Christopher M. Elvin.

Electronic supplementary material

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Canine colon anastomosis procedure using ILS stapler and sutures and subsequent sealing of the site using photopolymerised gelatin sealant (MP4 32.7 mb)

Movie 1

Canine colon anastomosis procedure using ILS stapler and sutures and subsequent sealing of the site using photopolymerised gelatin sealant (MP4 32.7 mb)

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Vuocolo, T., Haddad, R., Edwards, G.A. et al. A Highly Elastic and Adhesive Gelatin Tissue Sealant for Gastrointestinal Surgery and Colon Anastomosis. J Gastrointest Surg 16, 744–752 (2012). https://doi.org/10.1007/s11605-011-1771-8

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Keywords

  • Tissue sealant
  • Gelatin
  • Crosslinking
  • Photopolymerisation
  • Gastrointestinal anastomosis
  • Dehiscence