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Current Medical Science

, Volume 38, Issue 6, pp 982–988 | Cite as

Dual Convergence of Facial Nerve Branches Innervating Whisker Pad in Rats

  • Lin-li Yao
  • E Zhang
  • Chun-li Li
  • Qi Yao
  • Chang Huang
  • Xi Huang
  • Pei Chen
Article
  • 6 Downloads

Summary

The precise anatomy of the facial nerve branches innervating rat whisker pad and the distribution of their corresponding motor neurons in facial nucleus area were investigated. The extratemporal facial nerves of 6 rats were anatomically observed under a surgical microscope, and then the nerve specimens of facial nerve branches at 7 anatomical sites were taken and examined for the axons and myelin sheath using Luxol fast blue staining. The distribution of facial motor neurons innervating the facial branches was observed in 12 rats by retrograde labelling. The distal pes, a fusing architecture of the buccal and marginal mandibular branches, was found to furcate into superior, middle and inferior branches to innervate whisker pad. Histologically, the myelin sheath of each branch was morphologically consistent, and the nerve fiber bundles of facial nerve branches became increasingly thinner and scattered, particularly after crossing the distal pes site and innervating the whisker pad. The facial motor neurons innervating the buccal and marginal mandibular branches were clearly distributed in similar regions in facial nucleus. This study confirmed the highly spatial synergy between the buccal and marginal mandibular branches innervating the whisker pad from extratemporal anatomy and distribution of facial motor neurons.

Key words

facial nerve whisker pad anatomy facial nucleus rats 

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References

  1. 1.
    Bermejo R, Houben D, Zeigler HP. Optoelectronic monitoring of individual whisker movements in rats. J Neurosci Methods, 1998,83(2):89–96Google Scholar
  2. 2.
    Bermejo R, Szwed M, Friedman W, et al. One whisker whisking: unit recording during conditioned whisking in rats. Somatosens Mot Res, 2004, 21(3–4):183–187Google Scholar
  3. 3.
    Hadlock T, Kowaleski J, Lo D, et al. Functional assessments of the rodent facial nerve: a synkinesis model. Laryngoscope, 2008,118(10):1744–1749Google Scholar
  4. 4.
    Heaton JT, Kowaleski JM, Bermejo R, et al. A system for studying facial nerve function in rats through simultaneous bilateral monitoring of eyelid and whisker movements. J Neurosci Methods, 2008,171(2):197–206Google Scholar
  5. 5.
    Drfl J. The innervation of the mystacial region of the white mouse: a topographical study. J Anat, 1985,142:173–184Google Scholar
  6. 6.
    Semba K, Egger MD. The facial “motor” nerve of the rat: control of vibrissal movement and examination of motor and sensory components. J Comp Neurol, 1986,247:144–158Google Scholar
  7. 7.
    Yetiser S, Kahraman E, Satar B, et al. Anatomy of the Extratemporal Facial Nerve in Rats. Mediterr J Otol, 2005,1:117–121Google Scholar
  8. 8.
    Skouras E, Merkel D, Grosheva M, et al. Manual stimulation, but not acute electrical stimulation prior to reconstructive surgery, improves functional recovery after facial nerve injury in rats. Restor Neurol Neurosci, 2009,27(3):237–251Google Scholar
  9. 9.
    Henstrom D, Hadlock T, Lindsay R, et al. The convergence of facial nerve branches providing whisker pad motor supply in rats: implications for facial reanimation study. Muscle Nerve, 2012,45(5):692–697Google Scholar
  10. 10.
    Chen P, Knox CJ, Yao L, et al. The effects of venous ensheathment on facial nerve repair in the rat. Laryngoscope, 2017,127(7):1558–1564Google Scholar
  11. 11.
    Chen P, Song J, Luo LH, et al. Abnormal motor reflexes and dormant facial motor neurons in rats with facialfacial anastomosis. J Int Med Res, 2009,37(3):705–716Google Scholar
  12. 12.
    Chen P, Wang P, Chen G, et al. Study on remodeling of astrocytes in facial neuclus after peripheral injury. J Huazhong Univ Sci Technolog Med Sci, 2005,25(6):726–728Google Scholar
  13. 13.
    Martin MR, Lodge D. Morphology of the facial nucleus of the rat. Brain, 1977,123(1):1–12Google Scholar
  14. 14.
    Weinberg JS, Kleiss IJ, Knox CJ, et al. The Dilator Naris Muscle as a Reporter of Facial Nerve Regeneration in a Rat Model. Ann Plast Surg, 2016,76(1):94–98Google Scholar
  15. 15.
    Seitz M, Grosheva M, Skouras E, et al. Poor functional recovery and muscle polyinnervation after facial nerve injury in fibroblast growth factor-2-/-mice can be improved by manual stimulation of denervated vibrissal muscles. Neuroscience, 2011,182(1):241–247Google Scholar
  16. 16.
    Yamada H, Hato N, Murakami S, et al. Facial synkinesis after experimental compression of the facial nerve comparing intratemporal and extratemporal lesions. Laryngoscope, 2010,120(5):1022–1027Google Scholar
  17. 17.
    Hinrichsen CF, Watson CD. The facial nucleus of the rat: representation of facial muscles revealed by retrograde transport of horseradish peroxidase. Anat Rec, 1984,209(3):407–415Google Scholar
  18. 18.
    Ashwell KW. The adult mouse facial nerve nucleus: Morphology and musculotopic organization. J Anat, 1982,135(3):531–538Google Scholar
  19. 19.
    Choi D, Li D, Raisman G. Fluorescent retrograde neuronal tracers that label the rat facial nucleus: a comparison of Fast Blue, Fluoro-ruby, Fluoroemerald, Fluoro-Gold and DiI. J Neurosci Methods, 2002,117(2):167–172Google Scholar

Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Lin-li Yao
    • 1
  • E Zhang
    • 1
  • Chun-li Li
    • 1
  • Qi Yao
    • 1
  • Chang Huang
    • 1
  • Xi Huang
    • 1
  • Pei Chen
    • 1
  1. 1.Department of Otolaryngology, Wuhan Hospital of Traditional Chinese and Western Medicine (Wuhan No. 1 Hospital), Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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