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Current Medical Science

, Volume 38, Issue 4, pp 648–655 | Cite as

γδ T Cells Contribute to the Outcome of Murine Fulminant Viral Hepatitis via Effector Cytokines TNF-α and IFN-γ

  • Di Wu
  • Wei-ming Yan
  • Hong-wu Wang
  • Da Huang
  • Xiao-ping Luo
  • Qin Ning
Article
  • 24 Downloads

Summary

The mechanisms involved in virus-induced severe hepatitis have not been fully elucidated. In this study, we investigated the role of gamma delta T cell receptors (γδ) T cells in the pathogenesis of fulminant viral hepatitis (FVH) induced by murine hepatitis virus strain 3 (MHV-3). The model of FVH was established by intraperitoneal injection of MHV-3 into Balb/cJ mice. The survival days of mice, and the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were examined. The proportions of γδ T cells in blood, spleen and liver, and cytokines secreted by hepatic γδ T cells were analyzed by flow cytometry. The function of hepatic γδ T cells was examined by cytotoxicity assay. Balb/cJ mice died in 3 to 6 days post MHV-3 infection, with severe hepatic necrosis and significant augmentation of serum ALT and AST levels. The proportions of γδ T cells in blood, spleen and liver were significantly increased post MHV-3 infection, while those of the early activating molecule CD69-expressing γδ T cells and productions of cytokines tumor necrosis factor-alpha (TNF-α) and interferon-γ (IFN-γ) increased remarkably in the liver. These highly activated liver γδ T cells were cytotoxic to MHV-3-infected hepatocytes in vitro and this effect of liver γδ T cells against hepatocytes might involve the TNF-α and IFN-γ pathway. These results demonstrated that γδ T cells might contribute to the pathogenesis of MHV-3-induced FVH through the effector cytokines TNF-α and IFN-γ.

Key words

fulminant viral hepatitis murine hepatitis virus strain 3 gamma delta T cell receptors T cells tumor necrosis factor-α interferon-γ 

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Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Di Wu
    • 1
  • Wei-ming Yan
    • 1
  • Hong-wu Wang
    • 1
  • Da Huang
    • 1
  • Xiao-ping Luo
    • 2
  • Qin Ning
    • 1
  1. 1.Department of Infectious Disease, Institute of Infectious Disease, Tongji HospitalHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Pediatrics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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