Summary
The normal range of oral mucosal cell apoptosis and proliferation rate through a larger sample of non-malnourished crowd was investigated, and the nutritional status of clinical patients was assessed. Of 194 clinical patients selected according to “NRS2002” guidance, there were 167 non-malnourished patients and 27 malnourished cases, respectively. Twelve patients with toxic reactions of grade III after postoperative chemotherapy (POC) were chosen. The oral mucosal epithelial apoptosis and proliferation rate were measured by using flow cytometry. The statistical significance was processed by using unpaired t-test. The results showed that there was no significant difference in gender, age and body weight between malnourished and non-malnourished groups. The normal range of oral mucosal epithelial apoptosis and the proliferation rate was (27.50±1.50)% and (15.12±1.68)% in non-malnourished patients, and that was (19.90±4.14)% and (6.66±5.83)% in the malnourished patients, respectively. It is concluded that the normal range of oral mucosa cell apoptosis and proliferation rate is achieved, which can not be influenced by gender, age, weight and other factors, and could be used as a sensitive and accurate index to assess the nutritional status of clinical patients.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Li JS. Past and future of clinical nutritional support. Chin J Pract Surg (Chinese), 2000,20(1):7–9
Jeejeebhoy KN, Detsky AS, Baker JP. Assessment of nutritional status. JPEN 1990,14(Suppl 5):193S–196S
Agradi E, Messina V, Campanella G, et al. Hospital malnutrition: incidence and prospective evaluation of general medical patients during hospitalization. Acta Vitaminol Enzymol, 1984,6(4):235–242
Weinsier RL, Hunker EM, Krumdieck CL, et al. Hospital malnutrition: a prospective evaluation of general medical patients during the course of hospitalization. Am J Clin Nutr, 1979,32(2):418–426
Hill GL, Pickford I, Young GA, et al. Malnutrition in surgical patients: an unrecognized problem. Lancet. 1977,1(8013):689–692
Zhou Y, Lou XL, Yang CY, et al. Relationship between the oral mucosa epithelial apoptosis rate and the patient’s nutritional status. Chin J Exp Surg (Chinese), 2006,6(23): 670–672
Fan MW. Oral Biology. Beijing: People’s Medical Publishing House, 1995:27–29, 110–116
Lou XL, Zhou Y, Hu JB, et al. Apoptosis and proliferation of oral mucosa epithelial cells affected by nutritional status. Chin J Clin Nutr (Chinese), 2006,14(1):3–6
Gong JP, Traganos F, Darzynkiewicz Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem, 1994,218(2):314–319
Shu D, Tao DD, Gong GJ. Reappraisal of the analysis of cell apoptosis with sub-G1 and TdT in flow cytometry. Chin J Exp Surg (Chinese), 2000,17(1):59–60
Chen XL, Cai DL, Hu TJ. Determination of prealbumin, transferrin, retino-binding-protein of serum by ELISA in healthy adults. Parenteral Enteral Nutr (Chinese), 1999, 6(1):38–40
Dudrick SJ. Metabolite in Surgical Patient. In: Sabiston DC, eds. Sabiston Textbook of Surgery, USA: Saunders Co., 1987,89–97
Li YX, Li JS. Enteral nutrition support progress. J Jiangsu Clin Med (Chinese), 2002,6(2):90–91
Gong JP. The impact of cellular and molecular biology on surgery. Tongji Yike Daxue Xuebao (Chinese), 1994,23(Suppl 1):20
Lautz HU, Selberg O, Korber J, et al. Protein-calorie malnutrition in liver cirrhosis. Clin Investig, 1992,70(6): 478–486
Rainey-Macdonald CG, Holliday RL, Wells GA, et al. Validity of a two variable nutritional index for use in selecting candidates for nutritional support. JPEN 1983,7: 15–20
Butters M, Stranb M, Kraft K, et al. Studies on nutritional status in general surgery patients by clinical, anthropometric and laboratory parameters. Nutrition, 1996,12: 405–410
Jiang ZM, Cai W. Clinical enteral and parenteral nutrition. Beijing: Science & Technology Literature Press, 2000, 174–175, 344–349
Windsor JA, Hill L. Weight loss with physiologic impairment: A basic indicator of surgical risk. Ann Surg, 1988,207(3):290–296
Girvent M, Maestro S, Hernandez R. Euthyroid sick syndrome, associated endocrine abnormalities and outcome in elderly patients undergoing emergency operation. Surgery 1998,123(5):560–567
Wilmore D. Gastrointestinal metabolic support. Chin J Clin Nutr (Chinese), 2001,9:139
Furst P. New horizons in nutrition. Chin J Clin Nutr (Chinese), 2002,10(2):74
Grant JP. Nutritional assessment in clinical practice. Nutr Clin Pract, 1986,1:314
Ireton Jones CS, Hasse JM. Comprehensive nutritional assessment: the dietitian’s contribution to the team effort. Nutrition, 1992,8(2):75–81
Starker PM, Gump FE, Askanazi J, et al. Serum albumin levels as an index of nutritional support. Surgery, 1982,91(2):194–199
Cavarocchi NC, Au FC, Dalal FR, et al. Rapid turnover protein as nutritional indicators. World J Surg, 1986,10(3): 468–472
Calamita A, Dichi I, Papini SJ, et al. Plasma levels of transthyetin and retinal-binding protein in child-A cirrhotic patients in relation to protein-calorie status and plasma amino acids, zinc, vitamin A, and plasma thyroid hormones. Arg Gastroenterol, 1997,34(3):139–147
Feldman M. The myth of serum albumin as a measure of nutritional status. Gastroenterology, 1990,99(6):1845–1851
Campisi J, Morreo G, Pardee AB. Kinetics of G1 transit following brief starvation for serum factors. Exp Cell Res, 1984,152(2):459–466
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gao, C., Hasan, O., Wei, X. et al. Assessment of nutritional status of clinical patients by determining normal range of oral mucosal apoptosis and proliferation rate. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 680–685 (2012). https://doi.org/10.1007/s11596-012-1017-3
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-012-1017-3