Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins

  • Qiuhan Tang (汤秋菡)
  • Rui Zhao
  • Qi Lu (陆琦)Email author
  • Guangyan Qing (卿光焱)Email author


A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3O4@TiO2 (Fe3O4@mTiO2) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3O4@mTiO2-AIPA was successfully utilized to separate α-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3O4@mTiO2-AIPA for application in phosphoprotein enrichment.

Key words

magnetic microsphere phosphoprotein separation α-casein 


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Copyright information

© Wuhan University of Technology and Springer-Verlag GmbH Germany, Part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Chemistry, Chemical Engineering and Life ScienceWuhan University of TechnologyWuhanChina
  2. 2.State Key Laboratory of Advanced Technology for Materials Synthesis and ProcessingWuhan University of TechnologyWuhanChina
  3. 3.Research & Development CenterJushi Group Co., LtdTongxiangChina

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