Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins
- 1 Downloads
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3O4@TiO2 (Fe3O4@mTiO2) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3O4@mTiO2-AIPA was successfully utilized to separate α-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3O4@mTiO2-AIPA for application in phosphoprotein enrichment.
Key wordsmagnetic microsphere phosphoprotein separation α-casein
Unable to display preview. Download preview PDF.
- Hou J, Xie Z, Xue P, et al. Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAP/DAHC Matrix[J]. J. Biomed. Biotechnol., 2010: 759 690Google Scholar