Abstract
Nanoparticles conjugated with antibody were designed as active drug delivery system to reduce the toxicity and side effects of drugs for acute myeloid leukemia (AML). Moreover, methotrexate (MTX) was chosen as model drug and encapsulate within folic acid modified carboxymethyl chitosan (FA-CMCS) nanoparticles through self-assembling. The chemical structure, morphology, release and targeting of nanoparticles were characterized by routine detection. It is demonstrated that the mean diameter is about 150 nm, the release rate increases with the decreasing of pH, the binding rate of CD33 antibody and FA-CMCS nanoparticles is about 5:2, and nanoparticles can effectively bind onto HL60 cells in vitro. The experimental results indicate that the FA-CMCS nanoparticles conjugated with antibody may be used as a potential pHsensitive drug delivery system with leukemic targeting properties.
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References
Pae HO, Seo WG, Kim NY, et al. Induction of Granulocytic Differentiation in Acute Promyelocytic Leukemia Cells (HL-60) by Watersoluble Chitosan Oligomer[J]. Leukemia Res., 2001, 25(4): 339–346
Sievers EL. Targeted Therapy of Acute Myeloid Leukemia with Monoclonal Antibody and Immunoconjugates[J]. Cancer Chemother Pharmacol., 2000, 46 Suppl: S18–S22
Choi KY, Chung H, Min KH, et al. Self-assembled Hyaluronic Acid Nanoparticles for Active Tumor Targeting[J]. Biomaterials, 2010, 31(1): 106–114
Chen NY, Hung AFH, Lin CJ, et al. Manipulating mIgD-expressing B Cells with Anti-migis-d Monoclonal Antibodies[J]. Mol Immunol., 2013, 53(3): 187–197
Sood N, Chaudhary DK, Singh A, et al. Monoclonal Antibody to Serum Immunoglobulins of Clarias Batrachus and Its Application in Immunoassays[J]. Gene, 2012, 511(2): 411–419
Andrews RG, Singer JW, Bernstein ID. Precursors of Colony-forming Cells in Humans can be Distinguished from Colony-forming Cells by Expression of the CD33 and CD34 Antigens and Light Scatter Properties[J]. J. Exp. Med., 1989, 169(5): 1721–1731
Siao SC, Li KJ, Hsieh SC, et al. Tamm-Horsfall Glycoprotein Enhances PMN Phagocytosis by Binding to Cell Surface-Expressed Lactoferrin and Cathepsin G That Activates MAP Kinase Pathway [J]. Molecules, 2011, 16(3): 2119–2134
Ji J, Wu D, Liu L, et al. Preparation, Evaluation, and In Vitro Release of Folic Acid Conjugated O-Carboxymethyl Chitosan Nanoparticles Loaded with Methotrexate[J]. J. Appl. Polym. Sci., 2012, 125(S2): E208–E215
Pfeffer M, Maurer M, Stadlmann J, et al. Intracellular Interactome of Secreted Antibody Fab Fragment in Pichia Pastorisreveals its Routes of Secretion and Degradation[J]. Appl. Microbiol. Biot., 2012, 93(6): 2503–2512
Tan Y, Liu CG. Preparation and Characterization of Self-assemblied Nanoparticles Based on Folic Acid Modified Carboxymethyl Chitosan[J]. J. Mater. Sci-Mater. M., 2011, 22(5): 1213–1220
Saboktakin MR, Tabatabaie RM, Maharramov A, et al. Synthesis and in Vitro Evaluation of Carboxymethyl Starch–Chitosan Nanoparticles as Drug Delivery System to the Colon[J]. Int J Biol Macromol., 2011, 48(3): 381–385
Yang SJ, Lin FH, Tsai KC, et al. Folic Acid-Conjugated Chitosan Nanoparticles Enhanced Protoporphyrin IX Accumulation in Colorectal Cancer Cells[J]. Bioconjugate Chem., 2010, 21(4): 679–689
Wang F, Zhang D, Duan C, et al. Preparation and Characterizations of a Novel Deoxycholic Acid-O-Carboxymethylated Chitosan-Folic Acid Conjugates and Self-aggregates[J]. Carbohyd. Polym., 2011, 84(3): 1192–1200
Mathew MZ, Mohan JC, Manzoor K, et al. Folate Conjugated Carboxymethyl Chitosan–Manganese Doped Zinc Sulphide Nanoparticles for Targeted Drug Delivery and Imaging of Cancer Cells[J]. Carbohyd Polym., 2010, 80(2): 442–448
Sahu SK, Mallick SK, Santra S, et al. In Vitro Evaluation of Folic Acid Modified Carboxymethyl Chitosan Nanoparticles Loaded with Doxorubicin for Targeted Delivery[J]. J. Mater. Sci. -Mater. M., 2010, 21(5): 1587–1597
Nor NM, Razak KA, Tan SC, et al. Properties of Surface Functionalized Iron Oxide Nanoparticles (Ferrofluid) Conjugated Antibody for Lateral Flow Immunoassay Application[J]. J. Alloy. Compd., 2012, 538: 100–106
Mercaderc JV, Esteve-Turrillas FA, Agulló C, et al. Antibody Generation and Immunoassay Development in Diverse Formats for Pyrimethanil Specific and Sensitive Analysis[J]. Analyst., 2012, 137: 5672–5679
Ulbrich K, Šubr V, Strohalm J, et al. Polymeric Drugs Based on Conjugates of Synthetic and Natural Macromolecules I. Synthesis and Physico-chemical Characterization[J]. J. Control Release., 2000, 64(1-3): 63–79
Liu P, Qin L, Wang Q, et al. cRGD-functionalized mPEG-PLGAPLL Nanoparticles for Imaging and Therapy of Breast Cancer[J]. Biomaterials, 2012, 33(28): 6739–6747
Zhou Y, Tian XL, Li YS, et al. Development of a Monoclonal Antibodybased Sandwich-type Enzyme-linked Immunosorbent Assay (ELISA) for Detection of Abrin in Food Samples[J]. Food Chem., 2012, 135(4): 2661–2665
Houot R, Kohrt H, Goldstein MJ, et al. Immunomodulating Antibodies and Drugs for the Treatment of Hematological Malignancies[J]. Cancer Metast Rev., 2011, 30(1): 97–109
Simard P, Leroux JC. In Vivo Evaluation of pH-Sensitive Polymer-Based Immunoliposomes Targeting the CD33 Antigen[J]. Mol. Pharm., 2010, 7(4): 1098–1107
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Funded by the National Natural Science Foundation of China (No. 50973088)
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Hu, Z., Zheng, H., Li, D. et al. Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 31, 446–453 (2016). https://doi.org/10.1007/s11595-016-1390-z
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DOI: https://doi.org/10.1007/s11595-016-1390-z