, Volume 25, Issue 7, pp 3461–3471 | Cite as

Antimicrobial properties of poly(propylene) carbonate/Ag nanoparticle-modified tamarind seed polysaccharide with composite films

  • Indira Devi M.P.
  • Nallamuthu N.Email author
  • Rajini N.Email author
  • Senthil Muthu Kumar T.
  • Suchart Siengchin
  • Varada Rajulu A.
  • Hariram N.
Original Paper


Completely biodegradable poly(propylene)carbonate-based composite films with 10 wt.% of tamarind seed polysaccharide (TSP) as filler were prepared by solution casting method. In these composite films, using TSP as the reducing agent, silver nanoparticles (AgNPs) were in situ generated using 1 to 5 mM aq. AgNO3 source solutions. The hybrid nanocomposite films were characterized by FTIR spectroscopy, X-ray diffraction, scanning electron microscopy (SEM), polarized optical microscopy (POM), tensile testing, thermogravimetric analysis, and antibacterial studies. From the SEM analysis, it was evident that the particle size of the AgNPs varied between 44 and 86 nm when 1 to 4 mM source solutions were used. But on the other hand, the particle size increased to 406 nm when 5 mM source solution was used indicating agglomeration of the AgNPs. The reinforcement of TSP enhanced the crystallinity of the poly(propylene carbonate) (PPC) matrix. The hybrid nanocomposite films exhibited enhanced tensile and thermal properties when compared with the PPC matrix. Further, the hybrid nanocomposites exhibited excellent antibacterial properties against Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Bacillus licheniformis (B. licheniformis) and Staphylococcus aureus (S. aureus) bacteria. These hybrid nanocomposites with excellent tensile and antibacterial properties can be potentially used for food packaging applications.


Poly (propylene) carbonate Tamarind seed polysaccharide Silver nanoparticles In situ generation Tensile strength Antibacterial activity 



The authors are thankful to the authorities of Kalasalingam Academy of Research and Education, Tamil Nadu, India, for supporting this research by providing fabrication facilities.

Funding information

Department of Science and Technology, India, provided funding through Young Scientist Start up Scheme YSS/2015/001353 project.


  1. 1.
    Lewis H (2008) Eco-design of food packaging materials. In: Chiellini E (ed) Environmentally compatible food packaging. Woodhead Publishing Limited, Cambridge, pp 238–262Google Scholar
  2. 2.
    Guo G, Xiang A, Tian H (2018) Thermal and mechanical properties of eco-friendly poly(vinyl alcohol) films with surface treated bagasse fibers. J Polym Environ 26(9):3949–3956Google Scholar
  3. 3.
    Lau OW, Wong SK (2000) Contamination in food from packaging material. J Chromatogr A 882:255–270Google Scholar
  4. 4.
    Duan J, Obi Reddy K, Ashok B, Cai J, Zhang L, Rajulu AV (2016) Effects of spent tea leaf powder on the properties and functions of cellulose green composite films. J Environ Chem Eng 4:440–448Google Scholar
  5. 5.
    Dai L, Qiu C, Xiong L, Sun Q (2015) Characterization of corn starch-based films reinforced with taro starch nanoparticles. Food Chem 174:82–88Google Scholar
  6. 6.
    Tian H, Yan Y, Rajulu AV, Xiang A, Luo X (2017) Fabrication and properties of polyvinyl alcohol/starch blend films: effect of composition and humidity. Int J Biol Macromol 96:518–523Google Scholar
  7. 7.
    Tian H, Xu G (2011) Processing and characterization of glycerol-plasticized soy protein plastics reinforced with citric acid-modified starch nanoparticles. J Polym Environ 19(3):582–588Google Scholar
  8. 8.
    Ashok B, Naresh S, Obi Reddy K, Madhukar K, Cai J, Zhang L, Rajulu AV (2014) Tensile and thermal properties of poly(lactic acid)/eggshell powder composite films. Int J Polym Anal Charact 19(3):245–255Google Scholar
  9. 9.
    Xiang HX, Chen SH, Cheng YH, Zhou Z, Zhu MF (2013) Structural characteristics and enhanced mechanical and thermal properties of full biodegradable tea polyphenol/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composite films. Express Polym Lett 7(9):778–786Google Scholar
  10. 10.
    Tian H, Wang K, Liu D, Yan Y, Xiang A, Rajulu AV (2017) Enhanced mechanical and thermal properties of poly (vinyl alcohol)/corn starch blends by nanoclay intercalation. Int J Biol Macromol 101:314–320Google Scholar
  11. 11.
    Chisholm MH, Zhou Z (2004) Concerning the mechanism of the ring opening of propylene oxide in the copolymerization of propylene oxide and carbon dioxide to give poly (propylene carbonate). J Am Chem Soc 126(35):11030–11039Google Scholar
  12. 12.
    Xia G, Obi Reddy K, Uma Maheswari C, Jayaramudu J, Zhang J, Zhang J, Rajulu AV (2015) Preparation and properties of biodegradable spent tea leaf powder/poly(propylene carbonate) composite films. Int J Polym Anal Charact 20(4):377–387Google Scholar
  13. 13.
    Senthil Muthu Kumar T, Rajini N, Tian H, Rajulu AV, Ayrilmis N, Siengchin S (2018) Improved mechanical and thermal properties of spent coffee bean particulate reinforced poly(propylene carbonate) composites. Part Sci Technol.
  14. 14.
    Feng Y, Ashok B, Madhukar K, Zhang J, Zhang J, Obi Reddy K, Rajulu AV (2014) Preparation and characterization of polypropylene carbonate bio-filler (eggshell powder) composite films. Int J Polym Anal Charact 19(7):637–647Google Scholar
  15. 15.
    Nornberg B, Borchardt E, Luinstra GA, Fromm J (2014) Wood plastic composites from poly(propylene carbonate) and poplar wood flour—mechanical, thermal and morphological properties. Eur Polym J 51:167–176Google Scholar
  16. 16.
    Jiang G, Zhang M, Feng J, Zhang S, Wang X (2017) High oxygen barrier property of poly(propylene carbonate)/polyethylene glycol nanocomposites with low loading of cellulose nanocrytals. ACS Sustain Chem Eng 5:11246–11254Google Scholar
  17. 17.
    Senthil Muthu Kumar T, Rajini N, Tian H, Rajulu AV, Jappes JTW, Siengchin S (2017) Development and analysis of biodegradable poly(propylene carbonate)/tamarind nut powder composite films. Int J Polym Anal Charact 22(5):415–423Google Scholar
  18. 18.
    Chaudhry Q, Scotter M, Blackburn J, Ross B, Boxall A, Castle L, Aitken R, Watkins R (2008) Applications and implications of nanotechnologies for the food sector. Food Addit Contam A 25(3):241–258Google Scholar
  19. 19.
    Jones CM, Hoek EMV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12(5):1531–1551Google Scholar
  20. 20.
    Echegoyen Y, Nerin C (2013) Nanoparticle release from nano-silver antimicrobial food containers. Food Chem Toxicol 62:16–22Google Scholar
  21. 21.
    Liu D, Yuan L, Xu H, Tian H, Xiang A (2018) PVA grafted POSS hybrid for high performance polyvinyl alcohol films with enhanced thermal, hydrophobic and mechanical properties. Polym Compos.
  22. 22.
    Birla SS, Tiwari VV, Gade AK, Ingle AP, Yadav AP, Rai MK (2009) Fabrication of silver nanoparticles by phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Lett Appl Microbiol 48(2):173–179Google Scholar
  23. 23.
    Li WR, Xie XB, Shi QS, Duan SS, Ouyang YS, Chen YB (2011) Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals 24(1):135–141Google Scholar
  24. 24.
    de Azeredo HMC (2013) Antimicrobial nanostructures in food packaging. Trends Food Sci Technol 30(1):56–69Google Scholar
  25. 25.
    Cushen M, Kerry J, Morris M, Cruz-Romero M, Cummins E (2012) Nanotechnologies in the food industry e recent developments, risks and regulation. Trends Food Sci Technol 24(1):30–46Google Scholar
  26. 26.
    Llorens A, Lloret E, Picouet P, Fernandez A (2012) Study of the antifungal potential of novel cellulose/copper composites as absorbent materials for fruit juices. Int J Food Microbiol 158(2):113–119Google Scholar
  27. 27.
    Muthulakshmi L, Rajini N, Varada Rajulu A, Siengchin S, Kathiresan T (2017) Synthesis and characterization of cellulose/silver nanocomposites from bioflocculant reducing agent. Int J Biol Macromol 103:1113–1120Google Scholar
  28. 28.
    Bagul M, Sonawane SK, Arya SS (2015) Tamarind seeds: chemistry, technology, applications and health benefits: a review. Indian Food Ind Mag 34(3):28–35Google Scholar
  29. 29.
    Xu CS, Tian CC, Zhang WD, Xing JW, Cai ZS, Ren Y, Xu WZ, Liu HZ (2014) Synthesis and properties of polypropylene carbonate polyol-based waterborne polyurethane. Adv Mater Res 936:58–62Google Scholar
  30. 30.
    Zheng F, Mi QH, Zhang K, Xu J (2016) Synthesis and characterization of poly(propylene carbonate)/ modified sepiolite nanocomposites. Polym Compos 37(1):21–27Google Scholar
  31. 31.
    Gopinath V, Mubarak Ali D, Priyadarshini S, Meera Priyadharsshini N, Thajuddin N, Velusamy P (2012) Biosynthesis of silver nanoparticles from Tribulus terrestris and antimicrobial activity: a novel biological approach. Colloids Surf B 96:69–74Google Scholar
  32. 32.
    Basavegowda N, Idhayadhulla A, Lee YR (2014) Preparation of Au and Ag nanoparticles using Artemisia annua and their in vitro antibacterial and tyrosinase inhibitory activities. Mater Sci Eng C 43:56–64Google Scholar
  33. 33.
    Bindhu MR, Umadevi M (2013) Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity. Spectrochim Acta A 101:184–190Google Scholar
  34. 34.
    Suvith VS, Philip D (2014) Catalytic degradation of methylene blue using biosynthesized gold and silver nanoparticles. Spectrochim Acta A 118:526–532Google Scholar
  35. 35.
    Kumar V, Yadav SK (2009) Plant mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84:151–157Google Scholar
  36. 36.
    Jeeva K, Thiyagarajan M, Elangovan V, Geetha N, Venkatachalam P (2014) Caesalpinia coriaria leaf extracts mediated biosynthesis of metallic silver nanoparticles and their antibacterial activity against clinically isolated pathogens. Ind Crop Prod 52:714–720Google Scholar
  37. 37.
    Aseer JR, Sankaranarayanasamy K (2017) Effect of fiber content on tensile retention properties of cellulose microfiber reinforced polymer composites for automobile application. IOP Con Ser-Mat Sci 272:012020Google Scholar
  38. 38.
    Senthil Muthu Kumar T, Rajini N, Jawaid M, Rajulu AV, Jappes JTW (2018) Preparation and properties of cellulose/tamarind nut powder green composites. J Nat Fibers 15(1):11–20Google Scholar
  39. 39.
    Petrosino JF, Galhardo RS, Morales LD, Rosenberg SM (2009) Stress-induced beta-lactam antibiotic resistance mutation and sequences of stationary-phase mutations in the Escherichia coli chromosome. J Bacteriol 191(19):5881–5889Google Scholar
  40. 40.
    Webber MA, Piddock LJV (2002) The importance of efflux pumps in bacterial antibiotic resistance. J Antimicrob Chemother 51(1):9–11Google Scholar
  41. 41.
    Nikaido H (2009) Multidrug resistance in Bacteria. Annu Rev Biochem 78:119–146Google Scholar
  42. 42.
    Paramasivam P, Mudili V, Marriappan A, Periyasamy V, Kadirvelu K, Ramasamy R (2015) Biological synthesis and characterization of silver nanoparticles using Eclipta alba leaf extract and evaluation of its cytotoxic and antimicrobial potential. Bull Mater Sci 38(4):965–973Google Scholar
  43. 43.
    Ajitha B, Reddy AKY, Reddy SP (2014) Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their inborn antimicrobial activity. Spectrochim Acta A 121:164–172Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Indira Devi M.P.
    • 1
  • Nallamuthu N.
    • 1
    Email author
  • Rajini N.
    • 2
    Email author
  • Senthil Muthu Kumar T.
    • 2
    • 3
  • Suchart Siengchin
    • 3
  • Varada Rajulu A.
    • 4
  • Hariram N.
    • 5
  1. 1.Department of PhysicsKalasalingam Academy of Research and EducationKrishnankoilIndia
  2. 2.Department of Mechanical EngineeringKalasalingam Academy of Research and EducationKrishnankoilIndia
  3. 3.Department of Mechanical and Process Engineering, The Sirindhorn International Thai German Graduate School of Engineering (TGGS)King Mongkut’s University of Technology North BangkokBangkokThailand
  4. 4.Centre for Composite Materials, International Research CentreKalasalingam Academy of Research and EducationKrishnankoilIndia
  5. 5.Department of BiotechnologyKalasalingam Academy of Research and EducationKrishnankoilIndia

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