, Volume 25, Issue 7, pp 3407–3412 | Cite as

Electrochemical impedimetric immunosensor based on host–guest interaction between β-cyclodextrin and ferrocene anchored to ionic liquid

  • Youming Shen
  • Guangyu ShenEmail author
Original Paper


The present study represents the fabrication of electrochemical impedimetric immunosensor based on host–guest interaction. The β-cyclodextrin self-assembled monolayer was first modified on the surface of Au electrode. Then, ionic liquids functionalized with both aldehyde and ferrocene groups were introduced on the electrode via host–guest interaction between β-cyclodextrin and ferrocene. Here, aldehyde groups were used to directly immobilize antibody, in which no additional chemical cross-linker is required. The electron transfer resistance before and after human immunoglobulin G attachment was monitored as a function of its concentration to perform the analysis. The proposed immunosensor had a good performance in a linear range of 0.1–50 ng mL−1 with a detection limit of 0.03 ng mL−1 (S/N = 3). The process of fabricating immunosensor was characterized using cyclic voltammetric and electrochemical impedance spectroscopy. The impedimetric immunosensor provided a simple and sensitive detection method for the specific determination of IgG in human serum.


β-Cyclodextrin Functionalized ionic liquids Host–guest interaction Impedimetric immunosensor Human immunoglobulin G 


Funding information

This work was supported by Hunan Provincial Natural Science Foundation of China (2016JJ6105).

Supplementary material

11581_2019_2877_MOESM1_ESM.docx (33 kb)
ESM 1. (DOCX 32.8 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Material EngineeringHunan University of Arts and ScienceChangdeChina

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