Electrochemical impedimetric immunosensor based on host–guest interaction between β-cyclodextrin and ferrocene anchored to ionic liquid
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The present study represents the fabrication of electrochemical impedimetric immunosensor based on host–guest interaction. The β-cyclodextrin self-assembled monolayer was first modified on the surface of Au electrode. Then, ionic liquids functionalized with both aldehyde and ferrocene groups were introduced on the electrode via host–guest interaction between β-cyclodextrin and ferrocene. Here, aldehyde groups were used to directly immobilize antibody, in which no additional chemical cross-linker is required. The electron transfer resistance before and after human immunoglobulin G attachment was monitored as a function of its concentration to perform the analysis. The proposed immunosensor had a good performance in a linear range of 0.1–50 ng mL−1 with a detection limit of 0.03 ng mL−1 (S/N = 3). The process of fabricating immunosensor was characterized using cyclic voltammetric and electrochemical impedance spectroscopy. The impedimetric immunosensor provided a simple and sensitive detection method for the specific determination of IgG in human serum.
Keywordsβ-Cyclodextrin Functionalized ionic liquids Host–guest interaction Impedimetric immunosensor Human immunoglobulin G
This work was supported by Hunan Provincial Natural Science Foundation of China (2016JJ6105).
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