Synthesis of graphene/carbon nanofiber for electrochemical determination of levodopa in the presence of uric acid
- 40 Downloads
Carbon nanofiber (CNF) was prepared by electrospinning using polypropylene and nickel was coated on the CNF by electroless plating. Then, graphene (Gr) was synthesized on the surface of nickel by chemical vapor deposition. After etching nickel, the Gr/CNF was obtained eventually and then used as a working electrode for the determination of levodopa in the presence of uric acid by cyclic voltammetry and differential pulse voltammetry. The morphology and structure were investigated by scanning electron microscopy and Raman spectroscopy, respectively. The results indicate that the electrode exhibits a high sensitivity of 0.26 μA·μM−1 and a low measured limit of detection of 1 μM for levodopa in the range of 1–60 μM. The electrode shows excellent selectivity, reproducibility, and stability. It was also applied to determine levodopa in the spiked human urine samples.
KeywordsCarbon nanofiber Graphene Electroless plating Chemical vapor deposition Levodopa Uric acid
This work is supported by the Natural Science Foundation of Heilongjiang Province (LC2015020), Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Personnel of China (2015192), the Innovative Talent Fund of Harbin city (2016RAQXJ185), and Science Funds for the Young Innovative Talents of HUST (201604).
- 2.Mazloum-Ardakani M, Taleat Z, Khoshroo A, Beitollahi H, Dehghani H (2012) Electrocatalytic oxidation and voltammetric determination of levodopa in the presence of carbidopa at the surface of a nanostructure based electrochemical sensor. Biosens Bioelectron 35(1):75–81. https://doi.org/10.1016/j.bios.2012.02.014 CrossRefGoogle Scholar
- 5.Yuan Q, Liu Y, Ye C, Sun H, Dai D, Wei Q, Lai G, Wu T, Yu A, Fu L, Chee K, Lin C (2018) Highly stable and regenerative graphene-diamond hybrid electrochemical biosensor for fouling target dopamine detection. Biosens Bioelectron 111(111):117–123. https://doi.org/10.1016/j.bios.2018.04.006 CrossRefGoogle Scholar
- 9.Yang YJ, Li W (2014) CTAB functionalized graphene oxide/multiwalled carbon nanotube composite modified electrode for the simultaneous determination of ascorbic acid, dopamine, uric acid and nitrite. Biosens Bioelectron 56(56):300–306. https://doi.org/10.1016/j.bios.2014.01.037 CrossRefGoogle Scholar
- 10.Yue HY, Zhang H, Huang S, Lin XY, Gao X, Chang J, Yao LH, Guo EJ (2017) Synthesis of ZnO nanowire arrays/3D graphene foam and application for determination of levodopa in the presence of uric acid. Biosens Bioelectron 89(Pt 1):592–597. https://doi.org/10.1016/j.bios.2016.01.078 CrossRefGoogle Scholar
- 11.Zhao D, Yu G, Tian K, Xu C (2016) A highly sensitive and stable electrochemical sensor for simultaneous detection towards ascorbic acid, dopamine, and uric acid based on the hierarchical nanoporous PtTi alloy. Biosens Bioelectron 82(82):119–126. https://doi.org/10.1016/j.bios.2016.03.074 CrossRefGoogle Scholar
- 15.Rezaei B, Shams-Ghahfarokhi L, Havakeshian E, Ensafi AA (2016) An electrochemical biosensor based on nanoporous stainless steel modified by gold and palladium nanoparticles for simultaneous determination of levodopa and uric acid. Talanta 158(158):42–50. https://doi.org/10.1016/j.talanta.2016.04.061 CrossRefGoogle Scholar
- 19.Kim C, Cho YJ, Yun WY, Ngoc BTN, Yang KS, Chang DR, Lee JW, Kojima M, Kim YA, Endo M (2007) Fabrications and structural characterization of ultra-fine carbon fibres by electrospinning of polymer blends. Solid State Commun 142(1–2):20–23. https://doi.org/10.1016/j.ssc.2007.01.030 CrossRefGoogle Scholar
- 29.Afsahi S, Lerner MB, Goldstein JM, Lee J, Tang X, Bagarozzi DA, Pan D, Locascio L, Walker A, Barron F, Goldsmith BR (2018) Novel graphene-based biosensor for early detection of Zika virus infection. Biosens Bioelectron 100(100):85–88. https://doi.org/10.1016/j.bios.2017.08.051 CrossRefGoogle Scholar
- 36.Eksin E, Zor E, Erdem A, Bingol H (2017) Electrochemical monitoring of biointeraction by graphene-based material modified pencil graphite electrode. Biosensors & Bioelectronics 92:207–14. https://doi.org/10.1016/j.bios.2017.02.016