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Design and Fabrication of a Silver Nanocluster-Based Aptasensor for Lysozyme Detection

  • Leila Safaee Ardekani
  • Tahereh Tohidi Moghadam
  • Peter Waaben ThulstrupEmail author
  • Bijan RanjbarEmail author
Article
  • 37 Downloads

Abstract

Lysozyme is a universal part of the innate immune system and is present in various bodily fluids. It is also vastly studied as a biomarker for a variety of diseases and is used in the food industry as a preservative. However, due to lack of a universal and precise detection method, new techniques are highly sought after for this purpose. Here, we introduce a silver nanocluster-based aptasensor to detect lysozyme with high sensitivity. The sensor consists of an RNA aptamer and a complementary DNA sequence with a silver nanocluster template. Silver nanoclusters were synthesized by reduction of silver nitrate in the presence of complementary DNA sequence and characterized by transmission electron microscopy, UV-Visible, fluorescence, and circular dichroism spectroscopy. Emission of the silver nanoclusters switched between low and high intensity based on the quenching ability of the aptamer for DNA-silver nanoclusters and the binding affinity of the aptamer for lysozyme. In the presence of the aptamer, DNA-silver nanoclusters show low fluorescence due to the hybridization of the aptamer, and hence disruption in the formation of DNA-silver nanoclusters. Upon addition of lysozyme, aptamer binding to the DNA-templated silver nanoclusters will be inhibited due to the stronger interaction between the aptamer and lysozyme. Presence of the free DNA template leads to a restored fluorescence signal of DNA-silver nanoclusters which is dependent upon the lysozyme concentration. By utilizing this strategy, the complementary DNA-templated silver nanoclusters sensor can detect concentrations of lysozyme lower than 10 nM via a signal in the visible spectral region. The proposed sensor presents valuable features such as low-cost, high sensitivity, easy fabrication, and convenient operation for detection of lysozyme.

Keywords

Lysozyme Silver nanocluster Aptamer Biosensor 

Abbreviations

Apt

RNA aptamer

cDNA

Complementary DNA

cDNA-Apt

Complementary-aptamer

AgNCs

Silver nanoclusters

HSA

Human serum albumin

Cyt C

Cytochrome C

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11468_2019_954_MOESM1_ESM.pdf (813 kb)
ESM 1 (PDF 813 kb)

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Authors and Affiliations

  1. 1.Department of Nanobiotechnology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of ChemistryUniversity of CopenhagenCopenhagenDenmark
  3. 3.Department of Biophysics, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran

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