A Speckle-Free Angular Interrogation SPR Imaging Sensor Based on Galvanometer Scan and Laser Excitation
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A speckle-free fast angular interrogation surface plasmon resonance imaging (SPRi) sensor based on a diode-pumped all-solid-state laser and galvanometer is reported in this work. A bidirectional scan using a galvanometer realizes the fast scanning of the incidence angle. The experimental results showed that the time needed for completing an SPR dip measurement was decreased to 0.5 s. And through cascading an immovable diffuser and two diffusers rotating in opposite directions, laser speckle was eliminated. The dynamic detection range and the sensitivity reached 4.6 × 10−2 and 1.52 × 10−6 refractive index unit (RIU), respectively, in a 2D array sensor when the angle scanning range was set as 7.5°. More importantly, the results demonstrated that the angular interrogation SPR imaging sensor scheme had the capability to perform fast and high-throughput detection of biomolecular interactions at 2D sensor arrays.
KeywordsSurface plasmon resonance imaging sensor Angular interrogation Speckle-free Biomolecule interaction
Youjun Zeng and Yonghong Shao designed the experiments; Youjun Zeng, Jie Zhou, and Lei Wang performed the experiments; Youjun Zeng, Junle Qu, and Xiaoping Xiao analyzed the data; Yonghong Shao and Xuejin Li contributed reagents/materials/analysis tools; Youjun Zeng, Jie Zhou, and Bruce Zhi Gao wrote the paper.
This work was partially supported by the National Key Research and Development Program of China (2017YFB0403804); Project from National Natural Science Foundation of China (61527827, 61775148); Guangdong Natural Science Foundation and Province Project (2017B020210006, 2018A030310544); and Shenzhen Science and Technology R&D and Innovation Foundation (JCYJ20160422151611496, GRCK2017042110420047).
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Conflict of Interest
The authors declare that they have no conflict of interest.
- 10.Drescher DG, Selvakumar D, Drescher MJ (2018) Advances in protein. Chem Struct Biol 110:1–30Google Scholar
- 32.Byopadhyay R, Gittings AS, Suh SS, Dixon PK, Durian DJ (2005) Speckle-visibility spectroscopy: A tool to study time-varying dynamics. Rev Sci Instrum 76:205–216Google Scholar