Quantitative Detection of Thiopurines by Inter-particle Distance-Dependent Properties of Gold Nanoparticles
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As thiopurines are the source of chemotherapeutic drug which is helpful in treating acute lymphoblastic leukaemia, so the proper quantification of different purines is essential. As plasmonic nanoparticles (NPs) reported as colorimetric sensor due to their inter-particle variation in the presence of biomolecules. Here we have synthesised four different sizes (8–30 nm) gold nanoparticles (AuNPs) and chose as the analytical tool for the quantification of different purines. The characterisation of synthesised AuNPs was done by using FT-IR, TEM, DLS, EDS and UV-Vis spectroscopy. They showed remarkable stability for 10–15 days in the presence of long-range pH (3–12) and high concentration of the salt solution (100 μl, 0.1 M NaCl). Study of SPR variation was done for the quantification of purines. It has been seen that as the particle size, the concentration of purine and pH of the solution varies then SPR peak ~ 521 nm of AuNPs undergoes red shift and intensity of existing peak get reduced with time. The appearance of this new peak at ~ 700 nm justified the sensitivity of AuNPs towards purines. It was observed that the larger size AuNPs (30 nm) is more sensitive for detecting different purines at very low concentration (10−7 M for 6-thioguanine and 6-mercaptopurine).
KeywordsSurface plasmon resonance Gold nanoparticles Aggregation Thiopurines Quantitative detection
The authors are thankful to BRNS-DAE (Grant No: 34/14/63/2014) and SERB-DST (Grant No: SB/FT/CS-178/2013) for financial assistance. We are also thankful to DST-FIST, Sprint Testing solutions-Mumbai and Thapar University for providing instrumental facilities.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 2.Al-Ghobashy MA, Hassan SA, Abdelaziz DH, Elhosseiny NM, Sabry NA, Attia AS, el-Sayed MH (2016) Development and validation of LC-MS/MS assay for the simultaneous determination of methotrexate, 6-mercaptopurine and its active metabolite 6-thioguanine in plasma of children with acute lymphoblastic leukemia: correlation with genetic polymorphism. J Chromatogr B Anal Technol Biomed Life Sci 1038:88–94. https://doi.org/10.1016/j.jchromb.2016.10.035 CrossRefGoogle Scholar
- 4.Sean CS (2009) Martindale: the complete drug reference, 34th edn. Pharmaceutical Press, London, 884–886Google Scholar
- 5.Oancea I, Png CW, Das I, Lourie R, Winkler IG, Eri R, Subramaniam N, Jinnah HA, McWhinney BC, Levesque JP, McGuckin MA, Duley JA, Florin THJ (2013) A novel mouse model of veno-occlusive disease provides strategies to prevent thioguanine-induced hepatic toxicity. Gut 62(4):594–605. https://doi.org/10.1136/gutjnl-2012-302274 CrossRefPubMedGoogle Scholar
- 7.Fishman M, Mrozek-Orlowski M (1999) Cancer Chemotherapy Guidelines and Recommendations for Practice, 2nd edn. Oncology Nursing Press Inc, Pittsburgh PA, pp 25Google Scholar
- 10.Mawatari H, Kato Y, Nishimura SI et al (1998) Reversed-phase high-performance liquid chromatographic assay method for quantitating 6-mercaptopurine and its methylated and non-methylated metabolites in a single sample. J Chromatogr B Biomed Appl 716(1-2):392–396. https://doi.org/10.1016/S0378-4347(98)00329-6 CrossRefGoogle Scholar
- 11.Keuzenkamp-Jansen CW, De Abreu RA, Bökkerink JPM, Trijbels JMF (1995) Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 672(1):53–61. https://doi.org/10.1016/0378-4347(95)00206-X CrossRefGoogle Scholar
- 12.Lennard L, Singleton HJ (1992) High-performance liquid chromatographic assay of the methyl and nucleotide metabolites of 6-mercaptopurine: quantitation of red blood cell 6-thioguanine nucleotide, 6-thioinosinic acid and 6-methylmercaptopurine metabolites in a single sample. J Chromatogr B Biomed Sci Appl 583(1):83–90. https://doi.org/10.1016/0378-4347(92)80347-S CrossRefGoogle Scholar
- 18.Lubomirsky I, Wang TY, Gartsman K et al (2000) Biologically programmed nanoparticle assembly. Adv Mater 12(2):147–150. https://doi.org/10.1002/(SICI)1521-4095(200001)12:2<147::AID-ADMA147>3.0.CO;2-U CrossRefGoogle Scholar
- 20.Bright RM, Walter DG, Musick MD, Jackson MA, Allison KJ, Natan MJ (1996) Chemical and electrochemical Ag deposition onto preformed Au colloid monolayers: approaches to uniformly-sized surface features with Ag-like optical properties. Langmuir 12(3):810–817. https://doi.org/10.1021/la950429h CrossRefGoogle Scholar
- 24.Thanh NTK, Rosenzweig Z (2002) Development of an aggregation-based immunoassay for anti-protein A using gold nanoparticles. Abstr Pap Am Chem Soc 223:U74–U74Google Scholar
- 26.Musick MD, Keating CD, Lyon LA, Botsko SL, Peña DJ, Holliway WD, McEvoy TM, Richardson JN, Natan MJ (2000) Metal films prepared by stepwise assembly. 2. Construction and characterization of colloidal Au and Ag multilayers. Chem Mater 12(10):2869–2881. https://doi.org/10.1021/cm990714c CrossRefGoogle Scholar
- 33.Physik ADER, Vol N (1908) Beiträge zur Optik trüber Medien, speziell kolloidaller Metallösungen; von Gustav Mie. Ann Phys 25:1–52Google Scholar
- 37.Olesen KM, Hansen SH, Sidenius U, Schmiegelow K (2008) Determination of leukocyte DNA 6-thioguanine nucleotide levels by high-performance liquid chromatography with fluorescence detection. J Chromatogr B Anal Technol Biomed Life Sci 864(1-2):149–155. https://doi.org/10.1016/j.jchromb.2008.02.007 CrossRefGoogle Scholar
- 42.Ensafi AA, Karimi-Maleh H (2010) Modified multiwall carbon nanotubes paste electrode as a sensor for simultaneous determination of 6-thioguanine and folic acid using ferrocenedicarboxylic acid as a mediator. J Electroanal Chem 640(1-2):75–83. https://doi.org/10.1016/j.jelechem.2010.01.010 CrossRefGoogle Scholar