Quantitative Detection of Thiopurines by Inter-particle Distance-Dependent Properties of Gold Nanoparticles
As thiopurines are the source of chemotherapeutic drug which is helpful in treating acute lymphoblastic leukaemia, so the proper quantification of different purines is essential. As plasmonic nanoparticles (NPs) reported as colorimetric sensor due to their inter-particle variation in the presence of biomolecules. Here we have synthesised four different sizes (8–30 nm) gold nanoparticles (AuNPs) and chose as the analytical tool for the quantification of different purines. The characterisation of synthesised AuNPs was done by using FT-IR, TEM, DLS, EDS and UV-Vis spectroscopy. They showed remarkable stability for 10–15 days in the presence of long-range pH (3–12) and high concentration of the salt solution (100 μl, 0.1 M NaCl). Study of SPR variation was done for the quantification of purines. It has been seen that as the particle size, the concentration of purine and pH of the solution varies then SPR peak ~ 521 nm of AuNPs undergoes red shift and intensity of existing peak get reduced with time. The appearance of this new peak at ~ 700 nm justified the sensitivity of AuNPs towards purines. It was observed that the larger size AuNPs (30 nm) is more sensitive for detecting different purines at very low concentration (10−7 M for 6-thioguanine and 6-mercaptopurine).
KeywordsSurface plasmon resonance Gold nanoparticles Aggregation Thiopurines Quantitative detection
The authors are thankful to BRNS-DAE (Grant No: 34/14/63/2014) and SERB-DST (Grant No: SB/FT/CS-178/2013) for financial assistance. We are also thankful to DST-FIST, Sprint Testing solutions-Mumbai and Thapar University for providing instrumental facilities.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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