, Volume 13, Issue 5, pp 1585–1594 | Cite as

Enhanced Thermal Stability and Biocompatibility of Gold Nanorods by Graphene Oxide

  • Vahid ShirshahiEmail author
  • Shadie Hatamie
  • Seyed Nasrollah Tabatabaei
  • Marzieh Salimi
  • Reza SaberEmail author


In the present study, the effect of nanosized graphene oxide layer on thermal stability and biocompatibility of gold nanorods has been examined. The graphene oxide-wrapped gold nanorods were prepared by electrostatic interaction between negatively charged graphene oxide and positively charged nanorods. The resulting nanohybrids were then heated at different time intervals to 95 °C in a water bath to assess the effect of heat on the rods morphology. The structural changes in gold nanorods were monitored via UV-Vis spectroscopy measurements and transmission electron microscopy images. In similar experiments, the graphene oxide used to wrap gold nanorods was reduced by ascorbic acid in a 95 °C water bath. Our results indicate that while bare gold nanorods are highly vulnerable to elevated temperatures, graphene oxide and reduced graphene oxide-coated gold nanorods remain thermally stable with no structural changes. We also confirmed that the enhanced thermal stability is highly dependent on the concentration of deposited graphene oxide available on the surface of the gold nanorods. In addition, we performed an MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazoliumbromide) assay to make a comparison between the cytotoxicity of the nanohybrids and their primary building blocks on human dermal fibroblast cells as a normal cell line. We found evidence that graphene oxide can enhance the biocompatibility of the rods through covering toxic chemicals on the surface of them.

Graphical Abstract


Nanohybrids Gold nanorods Graphene oxide Thermal stability Biocompatibility 


Author Contributions

All authors have given approval to the final version of the manuscript.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Medical Nanotechnology, School of Advanced Medical TechnologiesTehran University of Medical SciencesTehranIran
  2. 2.Institute for Nanoscience and Nanotechnology (INST)Sharif University of TechnologyTehranIran
  3. 3.Department of Pharmacology, Sainte-Justine Research CenterUniversité de MontréalMontrèalCanada
  4. 4.Department of Medical Physics & Biomedical Engineering, School of MedicineTehran University of Medical SciencesTehranIran
  5. 5.Research Center of Science and Technology in Medicine, RCSTIMTehran University of Medical SciencesTehranIran

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