Acta Geotechnica

, Volume 14, Issue 3, pp 627–638 | Cite as

Study of the effect of temperature on microbially induced carbonate precipitation

  • Xiaohao Sun
  • Linchang MiaoEmail author
  • Tianzhi Tong
  • Chengcheng Wang
Research Paper


Temperature is a key factor that contributes to microbially induced calcium carbonate precipitation. At low temperatures, low enzyme activity results in a lack of calcium precipitation. In this study, Sporosarcina pasteurii and Bacillus megaterium were compared. Firstly, the optical density curves and enzyme activity curves of both bacteria were obtained during 48-h culture. Then, optical density, enzyme activity, and productive rates for calcium carbonate were measured to analyze the influence of temperature. Finally, the effect of urea concentration on carbonate precipitation was studied by changing the urea concentration that was added during inoculation. The obtained results showed that at high temperature, the growth rate of B. megaterium was close to that of S. pasteurii, while the opposite result was found at low temperature. The urease activities of B. megaterium were similar at different temperature conditions. At high temperature, B. megaterium showed lower enzyme activity, while at low temperature, it surpassed that of S. pasteurii. The same results were found for enzyme activity and for the precipitation rates of calcium carbonate. The addition of urea to the medium increased precipitation rates, and higher urea concentrations increased the obtained precipitation rates. With 20 g/L urea, the precipitation rate of B. megaterium at 15 °C matched that without urea addition at 30 °C. Therefore, adding urea to the medium at the time of inoculation can effectively overcome the low calcium precipitation at low temperature and enable subsequent low-temperature engineering applications.


Bacillus megaterium Carbonate precipitation Low temperature Sporosarcina pasteurii Urea addition 



This work was supported by the National Natural Science Foundation of China (No. 51578147) and Scientific Research Foundation of Graduate School of Southeast University (No. YBJJ1846). This work was also supported by “the Fundamental Research Funds for the Central Universities” and “Postgraduate Research and Practice Innovation Program of Jiangsu Province” (No. KYCX18_0107). The authors thank the valuable comments from the reviewers.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiaohao Sun
    • 1
  • Linchang Miao
    • 1
    Email author
  • Tianzhi Tong
    • 1
  • Chengcheng Wang
    • 1
  1. 1.Institute of Geotechnical EngineeringSoutheast UniversityNanjingChina

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