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In vivo assessment of hepatotoxicity, nephrotoxicity and biodistribution using 3-aminopropyltriethoxysilane-coated magnetic nanoparticles (APTS-MNPs) in ICR mice

  • Article
  • Materials Science
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Chinese Science Bulletin

Abstract

The biocompatibility and biodistribution of magnetic nanoparticles (MNPs) in vivo are essential to ensure their safely clinical application. We have studied these aspects with our 3-aminopropyltriethoxysilane-coated magnetic nanoparticles (APTS-MNPs) formulation, which can be used as magnetic induction hyperthermia media. Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice. Liver and kidney functions were tested. Heart, liver, spleen, lung, kidney, testis, and brain were sectioned for pathological analysis. Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues. Serum showed an increase in AST and LDH following APTS-MNPs injection. Histological analyses of selected tissues showed no obvious abnormal changes. In conclusion, APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81071885). The authors wish to acknowledge the assistance of Professor Tiande Zhao for ongoing helpful discussions and advice on experiments analysis.

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Authors and Affiliations

  1. Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing, 100049, China

    Xiaowen Wang & Jintian Tang

  2. Department of Biological Pharmaceuticals, Beijing University of Chinese Medicine, Beijing, 100029, China

    Jieying Zhang, Xin Yang, Zhenghai Tang, Yanwen Hu & Benke Chen

  3. Key Laboratory of Particle & Radiation Imaging, Tsinghua University, Ministry of Education, Beijing, 100084, China

    Benke Chen & Jintian Tang

Authors
  1. Xiaowen Wang
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  2. Jieying Zhang
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  3. Xin Yang
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  4. Zhenghai Tang
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  5. Yanwen Hu
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  6. Benke Chen
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  7. Jintian Tang
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Corresponding author

Correspondence to Jintian Tang.

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Xiaowen Wang, Jieying Zhang and Xin Yang have contributed equally to this work.

SPECIAL TOPIC: Nano Materials

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Wang, X., Zhang, J., Yang, X. et al. In vivo assessment of hepatotoxicity, nephrotoxicity and biodistribution using 3-aminopropyltriethoxysilane-coated magnetic nanoparticles (APTS-MNPs) in ICR mice. Chin. Sci. Bull. 59, 1800–1808 (2014). https://doi.org/10.1007/s11434-014-0296-4

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  • DOI: https://doi.org/10.1007/s11434-014-0296-4

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