Science China Technological Sciences

, Volume 62, Issue 9, pp 1616–1627 | Cite as

Application of phosphate-containing materials affects bioavailability of rare earth elements and bacterial community in soils

  • ShuLan Jin
  • ZhongJun Hu
  • BaiYing Man
  • HuaHua Pan
  • Xiao Kong
  • DeCai JinEmail author


The exploitation and smelting of rare earths can cause serious pollution to the farmland around the mining area. The rare earth elements (REEs) are absorbed by crops and enter the human body through the food chain, which threatens people’s health. The effects of four phosphorus-containing materials-calcium superphosphate (SSP), phosphate rock (PR), calcium magnesium phosphate (CMP) and bone charcoal (BC) on rice growth and bacterial community structure in REE mining area of Xinfeng County were studied by pot experiment. The soil solution was collected during rice transplanting and harvest periods respectively, the rice and soil samples were collected and sequenced. The concentrations of water-soluble REEs were measured by inductively coupled plasma mass spectrometry (ICP-MS), and bacteria in soil was deeply sequenced by the Illumina Miseq sequencing platform. PR, CMP and BC promoted the growth of rice, improved the biomass of rice roots, shoots and grains, and significantly reduced absorption and accumulation of REEs in rice roots, shoots and grains. SSP treatment reduced the pH value of soil, significantly improved the concentration of REE solution in soil and improved biomass of rice roots, shoots and grains, and significantly improved the concentration of REEs in grain. The effects of phosphorus-containing materials on the absorption and accumulation of 15 REEs in rice roots, shoots and grains were very different, and significantly influenced the soil bacterial community. SSP reduced richness and diversity of bacteria. CMP improved the diversity of soil bacteria, but reduced their richness. PR and BC treatment improved the richness and diversity of soil bacteria, and significantly increased the abundance of Bacillus. The results showed that adding PR, CMP and BC to soil in the REE mining area of Xinfeng can improve food security and eco-environmental quality, and hence, are potential restorative materials; SSP is not recommended for use in acidic soils.


calcium superphosphate phosphate rock calcium magnesium phosphorus bone charcoal rare earth elements rice bacterial community 


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Application of Phosphate-Containing Materials Affects Bioavailability of Rare Earth Elements and Bacterial Community in Soils


  1. 1.
    Wang L, Liang T. Accumulation and fractionation of rare earth elements in atmospheric particulates around a mine tailing in Baotou, China. Atmos Environ, 2014, 88: 23–2.CrossRefGoogle Scholar
  2. 2.
    Wiche O, Kummer N A, Heilmeier H. Interspecific root interactions between white lupin and barley enhance the uptake of rare earth elements (REEs) and nutrients in shoots of barley. Plant Soil, 2016, 402: 235–24.CrossRefGoogle Scholar
  3. 3.
    Wang L, Liang T, Kleinman P J A, et al. An experimental study on using rare earth elements to trace phosphorous losses from nonpoint sources. Chemosphere, 2011, 85: 1075–107.CrossRefGoogle Scholar
  4. 4.
    Liao S G. Study on the development strategy of Jiangxi rare earth industry. Dissertation of Masteral Degree. Nanchang: Nanchang University, 2011Google Scholar
  5. 5.
    Jin S L, Hu Z J, Qiu Q P, et al. Effects of phosphate amendments on the concentrations of rare earth elements in soil solution (in Chinese). Chin J Ecol, 2018, 37: 1693–170.Google Scholar
  6. 6.
    Jin S L, Huang Y Z, Hu Y, et al. Effects of bone char, phosphate rock and modifying agent on leaching of rare earth elements in soil (in Chinese). Acta Sci Circumst, 2016, 36: 3818–382.Google Scholar
  7. 7.
    Khan A M, Bakar N K A, Bakar A F A, et al. Chemical speciation and bioavailability of rare earth elements (REEs) in the ecosystem: A review. Environ Sci Pollut Res, 2017, 24: 22764–2278.CrossRefGoogle Scholar
  8. 8.
    Suja S, Fernandes L L, Rao V P. Distribution and fractionation of rare earth elements and Yttrium in suspended and bottom sediments of the Kali estuary, western India. Environ Earth Sci, 2017, 76: 17.CrossRefGoogle Scholar
  9. 9.
    Liang T, Li K, Wang L. State of rare earth elements in different environmental components in mining areas of China. Environ Monit Assess, 2014, 186: 1499–151.CrossRefGoogle Scholar
  10. 10.
    Jin S L, Huang Y Z, Hu Y, et al. Rare earth elements content and health risk assessment of soil and crops in typical rare earth mine area in Jiangxi Province. Acta Sci Circumst, 2014, 34: 3084–309.Google Scholar
  11. 11.
    Zhu W F, Xu S Q, Shao P P, et al. Investigation on intake allowance of rare earth: A study on bio-effect of rare earth in South Jiangxi. Chin Environ Sci, 1997, 17: 63–6.Google Scholar
  12. 12.
    Li X, Chen Z, Chen Z, et al. A human health risk assessment of rare earth elements in soil and vegetables from a mining area in Fujian Province, Southeast China. Chemosphere, 2013, 93: 1240–124.CrossRefGoogle Scholar
  13. 13.
    Marzec-Wróblewska U, Kamiński P, Łakota P, et al. Determination of rare earth elements in human sperm and association with semen quality. Arch Environ Contam Toxicol, 2015, 69: 191–20.CrossRefGoogle Scholar
  14. 14.
    Rim K T, Koo K H, Park J S. Toxicological evaluations of rare earths and their health impacts to workers: A literature review. Saf Health Work, 2013, 4: 12–2.CrossRefGoogle Scholar
  15. 15.
    Liu H, Wang J, Yang Z, et al. Serum proteomic analysis based on iTRAQ in miners exposed to soil containing rare earth elements. Biol Trace Elem Res, 2015, 167: 200–20.CrossRefGoogle Scholar
  16. 16.
    Zhang H, Feng J, Zhu W, et al. Chronic toxicity of rare-earth elements on human beings: Implications of blood biochemical indices in reehigh regions, South Jiangxi. Biol Trace Elem Res, 2000, 73: 1–1.CrossRefGoogle Scholar
  17. 17.
    Zhong W H, Cai Z C. Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay. Appl Soil Ecol, 2007, 36: 84–9.CrossRefGoogle Scholar
  18. 18.
    Beauregard M S, Hamel C, Atul-Nayyar C, et al. Long-term phosphorus fertilization impacts soil fungal and bacterial diversity but not AM fungal community in alfalfa. Microb Ecol, 2010, 59: 379–38.CrossRefGoogle Scholar
  19. 19.
    Yi B, Zhang Q, Gu C, et al. Effects of different fertilization regimes on nitrogen and phosphorus losses by surface runoff and bacterial community in a vegetable soil. J Soils Sediment, 2018, 18: 3186–319.CrossRefGoogle Scholar
  20. 20.
    Teufel A G, Li W, Kiss A J, et al. Impact of nitrogen and phosphorus on phytoplankton production and bacterial community structure in two stratified Antarctic lakes: A bioassay approach. Polar Biol, 2017, 40: 1007–102.CrossRefGoogle Scholar
  21. 21.
    Tan H, Barret M, Mooij M J, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phoD phosphorus mineraliser group in pasture soils. Biol Fertil Soils, 2013, 49: 661–67.CrossRefGoogle Scholar
  22. 22.
    Lagos L M, Acuña J J, Maruyama F, et al. Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites. Biol Fertil Soils, 2016, 52: 1007–101.CrossRefGoogle Scholar
  23. 23.
    Liu M, Liu J, Chen X, et al. Shifts in bacterial and fungal diversity in a paddy soil faced with phosphorus surplus. Biol Fertil Soils, 2018, 54: 259–26.CrossRefGoogle Scholar
  24. 24.
    Hamel C, Hanson K, Selles F, et al. Seasonal and long-term resourcerelated variations in soil microbial communities in wheat-based rotations of the Canadian prairie. Soil Biol Biochem, 2006, 38: 2104–211.CrossRefGoogle Scholar
  25. 25.
    Huang J, Hu B, Qi K, et al. Effects of phosphorus addition on soil microbial biomass and community composition in a subalpine spruce plantation. Eur J Soil Biol, 2016, 72: 35–4.CrossRefGoogle Scholar
  26. 26.
    Shi Y, Lalande R, Ziadi N, et al. An assessment of the soil microbial status after 17 years of tillage and mineral P fertilization management. Appl Soil Ecol, 2012, 62: 14–2.CrossRefGoogle Scholar
  27. 27.
    Jiang Z W, Weng B Q, Huang Y F, et al. Effects of lanthanum on soil microorganism (in Chinese). J Chin Rare Earth, 2008, 26: 498–50.Google Scholar
  28. 28.
    Zhou J, Xia B, Treves D S, et al. Spatial and resource factors influencing high microbial diversity in soil. Appl Environ Microbiol, 2002, 68: 326–33.CrossRefGoogle Scholar
  29. 29.
    Pol A, Barends T R M, Dietl A, et al. Rare earth metals are essential for methanotrophic life in volcanic mudpots. Environ Microbiol, 2014, 16: 255–26.CrossRefGoogle Scholar
  30. 30.
    Keltjens J T, Pol A, Reimann J, et al. PQQ-dependent methanol dehydrogenases: Rare-earth elements make a difference. Appl Microbiol Biotechnol, 2014, 98: 6163–618.CrossRefGoogle Scholar
  31. 31.
    Wang Y S, Hou X L, Cai L P, et al. Impacts of rare earth mining on soil bacterial community composition and biodiversity (in Chinese). J Environ Sci-China, 2017, 37: 3089–309.Google Scholar
  32. 32.
    Wang J H. Study on characteristic of soil microb exogenous rare earths accumulation area Baosteel Tailings Dam. Dissertation of Masteral Degree. Hohhot: Inner Mongolia Normal University, 2011Google Scholar
  33. 33.
    Ding S M, Liang T, Yan J C, et al. Fractionations of rare earth elements in plants and their conceptive model. Sci China Ser C, 2007, 50: 47–5.CrossRefGoogle Scholar
  34. 34.
    Jones R T, Robeson M S, Lauber C L, et al. A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses. ISME J, 2009, 3: 442–45.CrossRefGoogle Scholar
  35. 35.
    Fierer N, Lauber C L, Ramirez K S, et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. ISME J, 2012, 6: 1007–101.CrossRefGoogle Scholar
  36. 36.
    Bulgarelli D, Schlaeppi K, Spaepen S, et al. Structure and functions of the bacterial microbiota of plants. Annu Rev Plant Biol, 2013, 64: 807–83.CrossRefGoogle Scholar
  37. 37.
    Ward N L, Challacombe J F, Janssen P H, et al. Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Appl Environ Microbiol, 2009, 75: 2046.2056CrossRefGoogle Scholar
  38. 38.
    Chhabra S, Brazil D, Morrissey J, et al. Fertilization management affects the alkaline phosphatase bacterial community in barley rhizosphere soil. Biol Fertil Soils, 2013, 49: 31–3.CrossRefGoogle Scholar
  39. 39.
    Lauber C L, Hamady M, Knight R, et al. Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Appl Environ Microbiol, 2009, 75: 5111.5120CrossRefGoogle Scholar
  40. 40.
    Tarasov A L, Osipov G A, Borzenkov I A. Desulfovibrios from marine biofoulings at the South Vietnam coastal area and description of Desulfovibrio hontreensis sp. nov.. Microbiology, 2015, 84: 654–66.CrossRefGoogle Scholar
  41. 41.
    Geelhoed J S, Henstra A M, Stams A J M. Carboxydotrophic growth of Geobacter sulfurreducens. Appl Microbiol Biotechnol, 2016, 100: 997–100.CrossRefGoogle Scholar
  42. 42.
    Tanasupawat S, Takehana T, Yoshida S, et al. Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate). Int J Systatic Evolary MicroBiol, 2016, 19: 2813–281.Google Scholar
  43. 43.
    Berini F, Verce M, Ausec L, et al. Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens. Appl Microbiol Biotechnol, 2018, 102: 2425.2439CrossRefGoogle Scholar
  44. 44.
    Kulichevskaya I S, Suzina N E, Liesack W, et al. Bryobacter aggregatus gen. nov., sp. nov., a peat-inhabiting, aerobic chemo-organotroph from subdivision 3 of the Acidobacteria. Int J Syst Evol Micr, 2010, 60: 301–30.CrossRefGoogle Scholar
  45. 45.
    Chao Y, Liu W, Chen Y, et al. Structure, variation, and co-occurrence of soil microbial communities in abandoned sites of a rare earth elements mine. Environ Sci Technol, 2016, 50: 11481–1149.CrossRefGoogle Scholar
  46. 46.
    Nacke H, Thürmer A, Wollherr A, et al. Pyrosequencing-based assessment of bacterial community structure along different management types in German forest and grassland soils. PLoS ONE, 2011, 6: e17000Google Scholar
  47. 47.
    Leff J W, Jones S E, Prober S M, et al. Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe. Proc Natl Acad Sci USA, 2015, 112: 10967–1097.CrossRefGoogle Scholar
  48. 48.
    Ahn J H, Song J, Kim B Y, et al. Characterization of the bacterial and archaeal communities in rice field soils subjected to long-term fertilization practices. J Microbiol, 2012, 50: 754–76.CrossRefGoogle Scholar
  49. 49.
    Pereg L, de-Bashan L E, Bashan Y. Assessment of affinity and specificity of Azospirillum for plants. Plant Soil, 2016, 399: 389–41.CrossRefGoogle Scholar
  50. 50.
    Fukami J, Cerezini P, Hungria M. Azospirillum: Benefits that go far beyond biological nitrogen fixation. AMB Expr, 2018, 8: 1–1.CrossRefGoogle Scholar
  51. 51.
    Mignardi S, Corami A, Ferrini V. Evaluation of the effectiveness of phosphate treatment for the remediation of mine waste soils contaminated with Cd, Cu, Pb, and Zn. Chemosphere, 2012, 86: 354–36.CrossRefGoogle Scholar
  52. 52.
    Cleveland C C, Townsend A R, Schmidt S K. Phosphorus limitation of microbial processes in moist tropical forests: Evidence from shortterm laboratory incubations and field studies. Ecosystems, 2002, 5: 0680–069.CrossRefGoogle Scholar
  53. 53.
    Zhu F, Lu X, Liu L, et al. Phosphate addition enhanced soil inorganic nutrients to a large extent in three tropical forests. Sci Rep, 2015, 5: 792.Google Scholar
  54. 54.
    Lehmann J, Rillig M C, Thies J, et al. Biochar effects on soil biota—A review. Soil Biol Biochem, 2011, 43: 1812–183.CrossRefGoogle Scholar
  55. 55.
    McCormack S A, Ostle N, Bardgett R D, et al. Biochar in bioenergy cropping systems: Impacts on soil faunal communities and linked ecosystem processes. GCB Bioenergy, 2013, 5: 81–9.CrossRefGoogle Scholar
  56. 56.
    Gul S, Whalen J K, Thomas B W, et al. Physico-chemical properties and microbial responses in biochar-amended soils: Mechanisms and future directions. Agric Ecosyst Environ, 2015, 206: 46–5.CrossRefGoogle Scholar
  57. 57.
    Steinbeiss S, Gleixner G, Antonietti M. Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biol Biochem, 2009, 41: 1301–131.CrossRefGoogle Scholar
  58. 58.
    Ameloot N, De Neve S, Jegajeevagan K, et al. Short-term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils. Soil Biol Biochem, 2013, 57: 401–41.CrossRefGoogle Scholar
  59. 59.
    Gomez J D, Denef K, Stewart C E, et al. Biochar addition rate influences soil microbial abundance and activity in temperate soils. Eur J Soil Sci, 2014, 65: 28–3.CrossRefGoogle Scholar
  60. 60.
    Canbolat M Y, Bilen S, Çakmakçı R, et al. Effect of plant growthpromoting bacteria and soil compaction on barley seedling growth, nutrient uptake, soil properties and rhizosphere microflora. Biol Fertil Soils, 2006, 42: 350–35.CrossRefGoogle Scholar
  61. 61.
    Graber E R, Meller Harel Y, Kolton M, et al. Biochar impact on development and productivity of pepper and tomato grown in fertigated soilless media. Plant Soil, 2010, 337: 481–49.CrossRefGoogle Scholar
  62. 62.
    Kolton M, Meller Harel Y, Pasternak Z, et al. Impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants. Appl Environ Microbiol, 2011, 77: 4924–493.CrossRefGoogle Scholar
  63. 63.
    Chen Y P, Rekha P D, Arun A B, et al. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol, 2006, 34: 33–4.CrossRefGoogle Scholar
  64. 64.
    Zaidi A, Khan M, Ahemad M, et al. Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiol Imm H, 2009, 56: 263–28.CrossRefGoogle Scholar
  65. 65.
    Linu M S, Stephen J, Jisha M S. Phosphate solubilizing Gluconacetobacter sp., Burkholderia sp. and their potential interaction with cowpea (Vigna unguiculata (L.) Walp.). Int J Agric Res, 2009, 4: 79.87CrossRefGoogle Scholar
  66. 66.
    Oteino N, Lally R D, Kiwanuka S, et al. Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Front Microbiol, 2015, 6: 74.CrossRefGoogle Scholar

Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • ShuLan Jin
    • 1
  • ZhongJun Hu
    • 1
  • BaiYing Man
    • 1
  • HuaHua Pan
    • 1
  • Xiao Kong
    • 2
  • DeCai Jin
    • 2
    Email author
  1. 1.College of History, Geography and TourismShangrao Normal UniversityShangraoChina
  2. 2.Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijngChina

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