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Science China Earth Sciences

, Volume 63, Issue 1, pp 157–166 | Cite as

Antagonism between coral pathogen Vibrio coralliilyticus and other bacteria in the gastric cavity of scleractinian coral Galaxea fascicularis

  • Kaihao Tang
  • Waner Zhan
  • Yiqing Zhou
  • Tao Xu
  • Xiaoqing Chen
  • Weiquan Wang
  • Zhenshun Zeng
  • Yan WangEmail author
  • Xiaoxue WangEmail author
Research Paper
  • 35 Downloads

Abstract

Scleractinian corals host numerous microbial symbionts with different types of interactions. The gastric cavity of scleractinian coral, as a semiclosed subenvironment with distinct chemical characteristics (e.g., dissolved O2, pH, alkalinity, and nutrients), harbors a distinct microbial community and a diverse array of bacteria that can be pathogenic or beneficial. Galaxea fascicularis is one of the dominant massive scleractinian coral species on inshore fringing reefs in the northern South China Sea. Although the abundance of coral-associated bacteria has been investigated in G. fascicularis, less is known about the microorganisms in the gastric cavity. In this study, we specially isolated cultivable bacterial strains from the gastric cavity of G. fascicularis collected from Hainan Island using a noninvasive sampling approach. Among the 101 representative bacterial strains, one Vibrio coralliilyticus strain, SCSIO 43001, was found to be a temperature-dependent opportunistic pathogen of G. fascicularis. The antagonistic activity between the 100 strains and V. coralliilyticus SCSIO 43001 was tested using a modified Burkholder diffusion assay. Our results showed that V. coralliilyticus SCSIO 43001 inhibits the growth of Erythrobacterflavus and Sphingomonas yabuuchiae. Additionally, we found that three Pseudoalteromonas strains showed moderate to high anti-bacterial activity against V. coralliilyticus SCSIO 43001 and several other coral-associated Gram-negative bacterial strains. These results suggest that competition between the coral pathogen and other bacteria also occurs in the gastric cavity of coral, and Pseudoalteromonas strains in the gastric cavity of G. fascicularis may provide a protective role in the defense against co-inhabiting coral pathogens at elevated temperature.

Keywords

Coral pathogen Galaxea fascicularis Scleractinian coral Vibrio coralliilyticus 

Notes

Acknowledgements

This work was supported by the National Key R&D Program of China (Grant Nos. 2018YFC1406500 & 2017YFC0506303), the National Natural Science Foundation of China (Grant Nos. 41706172, 31625001 & 41376174) and the Hainan Provincial Key R&D (Grant No. ZDYF2018108).

Supplementary material

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Kaihao Tang
    • 1
  • Waner Zhan
    • 1
    • 2
  • Yiqing Zhou
    • 1
    • 2
  • Tao Xu
    • 3
  • Xiaoqing Chen
    • 3
  • Weiquan Wang
    • 1
    • 2
  • Zhenshun Zeng
    • 1
  • Yan Wang
    • 3
    Email author
  • Xiaoxue Wang
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Ocean CollegeHainan UniversityHaikouChina

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