Protein trafficking in plant cells: Tools and markers

  • Dongmei Zhu
  • Mengdi Zhang
  • Caiji Gao
  • Jinbo ShenEmail author


Eukaryotic cells consist of numerous membrane-bound organelles, which compartmentalize cellular materials to fulfil a variety of vital functions. In the post-genomic era, it is widely recognized that identification of the subcellular organelle localization and transport mechanisms of the encoded proteins are necessary for a fundamental understanding of their biological functions and the organization of cellular activity. Multiple experimental approaches are now available to determine the subcellular localizations and dynamics of proteins. In this review, we provide an overview of the current methods and organelle markers for protein subcellular localization and trafficking studies in plants, with a focus on the organelles of the endomembrane system. We also discuss the limitations of each method in terms of protein colocalization studies.


protein subcellular localization organelle markers endomembrane system 


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This work was supported by the National Natural Science Foundation of China (31970181), the Zhejiang Provincial Natural Science Foundation of China (R20C020001), the National Key Research and Development Program of China (2018YFD1000604) and the Zhejiang Agricultural and Forestry University Starting Funding (2018FR029). We apologize to colleagues whose work could not be included in this review because of space limitations.

Compliance and ethics

The author(s) declare that they have no conflict of interest.


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Dongmei Zhu
    • 1
  • Mengdi Zhang
    • 1
  • Caiji Gao
    • 2
  • Jinbo Shen
    • 1
    Email author
  1. 1.State Key Laboratory of Subtropical SilvicultureZhejiang Aamp;F UniversityHangzhouChina
  2. 2.Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life SciencesSouth China Normal University (SCNU)GuangzhouChina

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