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Science China Life Sciences

, Volume 62, Issue 8, pp 1028–1037 | Cite as

Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells

  • Tingting Lv
  • Kewu Jiang
  • Jiawen Wang
  • Nan Tang
  • Huaping DaiEmail author
  • Chen WangEmail author
Research Paper

Abstract

The aging of alveolar stem cells has been linked to many chronic lung diseases, including pulmonary fibrosis. However, the effects of aging on alveolar stem cells during homeostasis and post-injury alveolar repair have not been well characterized. Here we conducted a single-cell RNA sequencing (scRNA-seq) analysis of alveolar stem cells of 3-month-old and 12-month-old mice to characterize the aging effect on alveolar stem cells. Our results have shown that the transcriptomes of alveolar stem cells of 3-month-old and 12-month-old mice are not significantly different under the steady condition. However, after a bleomycin-induced lung injury, the alveolar stem cells of 12-month-old mice show enhanced inflammatory responses and decreased lipid metabolism. Our study suggests a close relationship among aging, lipid metabolism, inflammatory responses and chronic lung diseases.

Keywords

single-cell RNA sequencing (scRNA-seq) aging pulmonary alveolar stem cells inflammation response lipid metabolism 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81430001 and 81870056) and Beijing Major Science and Technology Projects (Z171100000417003 to N.T.).

Supplementary material

11427_2019_9583_MOESM1_ESM.pdf (397 kb)
Supplementary material, approximately 228 KB.

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pulmonary and Critical Care Medicine; Center of Respiratory Medicine, China-Japan Friendship Hospital; National Clinical Research Center for Respiratory DiseasesInstitute of Respiratory Medicine Chinese Academy of Medical SciencesBeijingChina
  2. 2.Graduate School of Peking Union Medical CollegeChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina
  3. 3.National Institute of Biological SciencesBeijingChina

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