Ambient temperature alters body size and gut microbiota of Xenopus tropicalis
- 16 Downloads
Temperature is important to determine physiological status of ectotherms. However, it is still not fully understood how amphibians and their symbiotic microbiota acclimate to ambient temperature. In this study, we investigated the changes of gut microbiota of Xenopus tropicalis at different temperatures under controlled laboratory conditions. The results showed that microbial communities were distinct and shared only a small overlap among froglet guts, culture water and food samples. Furthermore, the dominant taxa harbored in the gut exhibited low relative abundance in water and food. It indicates that bacterial taxa selected by amphibian gut were generally of low abundance in the external environment. Temperature could affect beta-diversity of gut microbiota in terms of phylogenetic distance, but it did not affect alpha diversity. The composition of gut microbiota was similar in warm and cool treatments. However, signature taxa in different temperature environments were identified. The relationships between temperature, gut microbiota and morphology traits of X. tropicalis revealed in this study help us to predict the consequences of environmental changes on ectothermic animals.
Unable to display preview. Download preview PDF.
This work was supported by the National Key Program of Research and Development, Ministry of Science and Technology of China (2016YFC0503200), Sichuan Province Science and Technology Project (2017SZ0004), the 13th Five-year Informatization Plan of Chinese Academy of Sciences (XXH13503-03-106), Open Fund of Key Laboratory of Environmental and Applied Microbiology CAS (KLCAS-2017-3, KLCAS-2016-03), and China Biodiversity Observation Networks (Sino BON).
- Bajaj, J.S., Hylemon, P.B., Ridlon, J.M., Heuman, D.M., Daita, K., White, M.B., Monteith, P., Noble, N.A., Sikaroodi, M., and Gillevet, P.M. (2012a). Colonic mucosal microbiome differs from stool microbiome in cirrhosis and hepatic encephalopathy and is linked to cognition and inflammation. Am J Physiol Gastrointest Liver Physiol 303, G675–G685.PubMedPubMedCentralCrossRefGoogle Scholar
- Bletz, M.C., Goedbloed, D.J., Sanchez, E., Reinhardt, T., Tebbe, C.C., Bhuju, S., Geffers, R., Jarek, M., Vences, M., and Steinfartz, S. (2016). Amphibian gut microbiota shifts differentially in community structure but converges on habitat-specific predicted functions. Nat Commun 7, 13699.PubMedPubMedCentralCrossRefGoogle Scholar
- Blooi, M., Pasmans, F., Rouffaer, L., Haesebrouck, F., Vercammen, F., and Martel, A. (2015b). Successful treatment of Batrachochytrium salamandrivorans infections in salamanders requires synergy between voriconazole, polymyxin E and temperature. Sci Rep 5, 11788.PubMedPubMedCentralCrossRefGoogle Scholar
- Carr, A.H., Amborski, R.L., Culley, D.D. Jr., and Amborski, G.F. (1976). Aerobic bacteria in the intestinal tracts of bullfrogs (Rana catesbeiana) maintained at low temperatures. Herpetologica 32, 239–244.Google Scholar
- Everard, A., Belzer, C., Geurts, L., Ouwerkerk, J.P., Druart, C., Bindels, L. B., Guiot, Y., Derrien, M., Muccioli, G.G., Delzenne, N.M., et al. (2013). Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proc Natl Acad Sci USA 110, 9066–9071.PubMedCrossRefGoogle Scholar
- Geurts, L., Lazarevic, V., Derrien, M., Everard, A., Van Roye, M., Knauf, C., Valet, P., Girard, M., Muccioli, G.G., François, P., et al. (2011). Altered gut microbiota and endocannabinoid system tone in obese and diabetic leptin-resistant mice: impact on apelin regulation in adipose tissue. Front Microbiol 2, 149.PubMedPubMedCentralCrossRefGoogle Scholar
- Li, H., Li, T., Yao, M., Li, J., Zhang, S., Wirth, S., Cao, W., Lin, Q., and Li, X. (2016). Pika gut may select for rare but diverse environmental bacteria. Front Microbiol 7, 1–7.Google Scholar
- Ribas, L., Li, M.S., Doddington, B.J., Robert, J., Seidel, J.A., Kroll, J.S., Zimmerman, L.B., Grassly, N.C., Garner, T.W.J., and Fisher, M.C. (2009). Expression profiling the temperature-dependent amphibian response to infection by Batrachochytrium dendrobatidis. PLoS ONE 4, e8408.PubMedPubMedCentralCrossRefGoogle Scholar