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Science China Life Sciences

, Volume 62, Issue 4, pp 579–593 | Cite as

Origination and evolution of orphan genes and de novo genes in the genome of Caenorhabditis elegans

  • Wenyu Zhang
  • Yuanxiao Gao
  • Manyuan Long
  • Bairong ShenEmail author
Research Paper
  • 70 Downloads

Abstract

Orphan genes that lack detectable homologues in other lineages could contribute to a variety of biological functions. However, their origination and function mechanisms remain largely unknown. Herein, through a comprehensive and systematic computational pipeline, we identified 893 orphan genes in the lineage of C. elegans, of which only a low fraction (0.9%) were derived from transposon elements. Six new protein-coding genes that de novo originated from non-coding DNA sequences in the genome of C. elegans were also identified. The authenticity and functionality of these orphan genes and de novo genes are supported by three lines of evidences, consisting of transcriptional data, and in silico proteomic data, and the fixation status data in wild populations. Orphan genes and de novo genes exhibited simple gene structures, such as, short in protein length, of fewer exons, and are frequently X-linked. RNA-seq data analysis showed these orphan genes are enriched with expression in embryo development and gonad, and their potential function in early development was further supported by gene ontology enrichment analysis results. Meanwhile, de novo genes were found to be with significant expression in gonad, and functional enrichment analysis of the co-expression genes of these de novo genes suggested they may be functionally involved in signaling transduction pathway and metabolism process. Our results presented the first systematic evidence on the evolution of orphan genes and de novo origin of genes in nematodes and their impacts on the functional and phenotypic evolution, and thus could shed new light on our appreciation of the importance of these new genes.

Keywords

Caenorhabditis elegans orphan genes de novo genes 

Notes

Acknowledgements

We are grateful to Li Zhang for providing helpful suggestions on de novo gene identification analysis. Computing was supported by EEgrid cluster of the University of Chicago. This work was supported by National Natural Science Foundation of China (31600670 to W. Zhang, 31670851 to B. Shen).

Supplementary material

11427_2019_9482_MOESM1_ESM.pptx (55 kb)
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Supplementary Table S2 Geography distribution of 40 Caenorhabditis elegans wild strains
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11427_2019_9482_MOESM5_ESM.xlsx (665 kb)
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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Wenyu Zhang
    • 1
    • 2
  • Yuanxiao Gao
    • 3
  • Manyuan Long
    • 2
    • 4
  • Bairong Shen
    • 1
    Email author
  1. 1.Institutes for Systems Genetics, West China HospitalSichuan UniversityChengduChina
  2. 2.Department of Ecology and Evolutionthe University of ChicagoChicagoUSA
  3. 3.Department of Evolutionary TheoryMax-Planck Institute for Evolutionary BiologyPlönGermany
  4. 4.Committee on Geneticsthe University of ChicagoChicagoUSA

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