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Science China Life Sciences

, Volume 62, Issue 6, pp 791–806 | Cite as

Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity

  • Gang Yang
  • Huanyu Wang
  • Mengyu Feng
  • Lei You
  • Lianfang Zheng
  • Taiping Zhang
  • Lin CongEmail author
  • Yupei ZhaoEmail author
Research Paper

Abstract

Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized both in vitro and in vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of mRNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells, indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer.

Keywords

pancreatic cancer high metastatic activity gene expression methylation 

Notes

Acknowledgements

This work was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS) (2016-12M-3-005 and 2016-I2M-1-001), PUMC Youth Fund and the Fundamental Research Funds for the Central Universities (2017320027), the National Natural Science Foundation of China (81772639), and the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2018PT32014).

Supplementary material

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Gang Yang
    • 1
  • Huanyu Wang
    • 1
  • Mengyu Feng
    • 1
  • Lei You
    • 1
  • Lianfang Zheng
    • 2
  • Taiping Zhang
    • 1
    • 3
  • Lin Cong
    • 1
    Email author
  • Yupei Zhao
    • 1
    Email author
  1. 1.Department of General Surgery, Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina
  2. 2.Department of Nuclear Medicine, Peking Union Medical HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina
  3. 3.Clinical Immunology CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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