Reduced β2-GPI is associated with increased platelet aggregation and activation in patients with prolonged isolated thrombocytopenia after allo-HSCT
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We aimed to measure platelet function and its relationship with β2-GPI in prolonged isolated thrombocytopenia (PT) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Fifty-six patients with PT and 60 allo-HSCT recipients without PT (non-PT controls) were enrolled. Platelet aggregation and activation, β2-GPI and anti-β2-GPI antibody levels, vWF antigen, and vWF activity were analyzed. The effect of β2-GPI on platelet aggregation was also measured ex vivo. Results showed that ADP-induced platelet aggregation significantly increased (39%±7.5% vs. 23%±8.5%, P=0.032), and the platelet expression of both CD62p (33.6%±11.6% vs. 8.5%±3.5%, P<0.001) and PAC-1 (42.4%±7.6% vs. 6.8%±2.2%, P<0.001) was significantly higher in patients with PT than in those without PT. Significantly lower β2-GPI levels (164.2±12 μg mL−1 vs. 234.2±16 μg mL−1, P<0.001), higher anti-β2-GPI IgG levels (1.78±0.46 U mL−1 vs. 0.94±0.39 U mL−1, P<0.001), and increased vWF activity (133.06%±30.50% vs. 102.17%±25.90%, P<0.001) were observed in patients with PT than in those without PT. Both ADP-induced platelet aggregation (n=116, r2=-0.5042, P<0.001) and vWF activity (n=116, r2=-0.2872, P<0.001) were negatively correlated with β2-GPI levels. In summary, our data suggested that platelet aggregation and activation were significantly higher in patients with PT than in those without PT, which might be associated with reduced β2-GPI levels. The reduced β2-GPI levels might be due to the existence of anti-β2-GPI IgG.
Keywordsβ2-GPI hematopoietic stem cell transplantation prolonged isolated thrombocytopenia platelet aggregation platelet activation
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This work was supported by the Key Program of National Natural Science Foundation of China (81730004), National Natural Science Foundation of China (81470343 and 81670116), Beijing Natural Science Foundation (7171013), Beijing Municipal Science and Technology Commission (Z171100001017084) and the National Key Research and Development Program of China (2017YFA0105500, 2017YFA0105503).
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