Glutamate and aspartate alleviate testicular/epididymal oxidative stress by supporting antioxidant enzymes and immune defense systems in boars
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Several potential oxidative agents have damaging effects on mammalian reproductive systems. This study was conducted to investigate the effects of glutamate (Glu) and aspartate (Asp) supplementation on antioxidant enzymes and immune defense systems in the outer scrotum of boars injected with H2O2. A total of 24 healthy boars were randomly divided into 4 treatment groups: control (basal diet, saline-treated), H2O2 (basal diet, H2O2-challenged outer scrotum (1 mL kg−1 BW)), Glu (basal diet +2% Glu, H2O2-challenged), and Asp (basal diet+2% Asp, H2O2-challenged). Our results showed that both Glu and Asp supplementation improved testicular morphology and decreased the genital index in the H2O2-treated boars. Glu and Asp administration increased the antioxidant enzyme activities and affected the testicular inflammatory cytokine secretion but had no effect on sex hormone levels. Furthermore, the mRNA expression of CAT, CuZnSOD, and GPx4 was altered in the testes and epididymis of boars treated with Asp and Glu. Glu and Asp supplementation also modulated the expression of TGF-β1, IL-10, TNF-α, IL-6 and IL-1β in the testis and epididymis. These results indicate that dietary Glu and Asp supplementation might enhance antioxidant capacity and regulate the secretion and expression of inflammatory cytokines to protect the testes and epididymis of boars against oxidative stress.
Englutamate aspartate boars oxidative stress inflammatory cytokines
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This work was supported by the National Science Foundation for Outstanding Young Scholars of Hunan Province (2019JJ30017), National Natural Science Foundation of China (31872371), Key Research and Development Programs of Hunan Province (2017NK2321), Changsha Science and Technology Key Program (kq1801058), the Science Foundation for Outstanding Youth Scholars of the Department of Hunan Provincial Education (18B012), the Earmarked Fund for China Agriculture Research System (CARS-35), the Open Fund of Key Laboratory of Agro-ecological Processes in Subtropical Region, Chinese Academy of Sciences (ISA2018204), the project of “Innovation Platform and Talents Program” of Hunan Provincial Science and Technology Department (2018RS3105), Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process (2018TP1031), the Project “2011 Collaborative Innovation Center of Hunan province” (2013, No.448), Science & Technology Innovation Talents of Hunan Province (2017TP1021 kc1704007).
- Albers, D.S., and Beal, M.F. (2000). Mitochondrial dysfunction and oxidative stress in aging and neurodegenerative disease. J Neural Transm Supp 59, 133–154.Google Scholar
- Duan, J., Yin, J., Wu, M., Liao, P., Deng, D., Liu, G., Wen, Q., Wang, Y., Qiu, W., Liu, Y., et al. (2014). Dietary glutamate supplementation ameliorates mycotoxin-induced abnormalities in the intestinal structure and expression of amino acid transporters in young pigs. PLoS ONE 9, e112357.CrossRefGoogle Scholar
- Foey, A.D., Parry, S.L., Williams, L.M., Feldmann, M., Foxwell, B.M.J., and Brennan, F.M. (1998). Regulation ofmonocyte IL-10 synthesis by endogenous IL-1 and TNF-alpha: Role of the p38 and p42/44 mitogen-activated protein kinases. J Immunol 160, 920–928.Google Scholar
- Ni, H., Lu, L., Deng, J., Fan, W., Li, T., and Yao, J. (2016). Effects of glutamate and aspartate on serum antioxidative enzyme, sex hormones, and genital inflammation in boars challenged with hydrogen peroxide. Mediat Inflamm 2016, 4394695.Google Scholar
- Puntel, R.L., Roos, D.H., Grotto, D., Garcia, S.C., Nogueira, C.W., and Batista Teixeira Rocha, J. (2007). Antioxidant properties of Krebs cycle intermediates against malonate pro-oxidant activity in vitro: A comparative study using the colorimetric method and HPLC analysis to determine malondialdehyde in rat brain homogenates. Life Sci 81, 51–62.CrossRefGoogle Scholar
- Sedha, S., Kumar, S., and Shukla, S. (2015). Role of oxidative stress in male reproductive dysfunctions with reference to phthalate compounds. Urol J 12, 2304–2316.Google Scholar
- Shafiee, M., Ahmadnezhad, M., Tayefi, M., Arekhi, S., Vatanparast, H., Esmaeili, H., Moohebati, M., Ferns, G.A., Mokhber, N., Arefhosseini, S.R., et al. (2018). Depression and anxiety symptoms are associated with prooxidant-antioxidant balance: A population-based study. J Affect Dis 238, 491–498.CrossRefGoogle Scholar