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Science China Life Sciences

, Volume 61, Issue 8, pp 975–977 | Cite as

Synthetic genomes engineered by SCRaMbLEing

  • Feng Zhang
  • Daniel F. Voytas
Insight
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References

  1. Blount, B.A., Gowers, G.O., Ho, J., Ledesma-Amaro, R., Jovicevic, D., McKiernan, R.M., Xie, Z.X., Li, B.Z., Yuan, J.Y., and Ellis, T. (2018). Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome. Nat Commun 9, 1932.CrossRefPubMedPubMedCentralGoogle Scholar
  2. Dymond, J.S., Richardson, S.M., Coombes, C.E., Babatz, T., Muller, H., Annaluru, N., Blake, W.J., Schwerzmann, J.W., Dai, J., Lindstrom, D. L., et al. (2011). Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature 477, 471–476.CrossRefPubMedPubMedCentralGoogle Scholar
  3. Gibson, D.G., Glass, J.I., Lartigue, C., Noskov, V.N., Chuang, R.Y., Algire, M.A., Benders, G.A., Montague, M.G., Ma, L., Moodie, M.M., et al. (2010). Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329, 52–56.CrossRefPubMedGoogle Scholar
  4. Hutchison, C.A., 3rd, Chuang, R.Y., Noskov, V.N., Assad-Garcia, N., Deerinck, T.J., Ellisman, M.H., Gill, J., Kannan, K., Karas, B.J., Ma, L., et al. (2016). Design and synthesis of a minimal bacterial genome. Science 351, aad6253.CrossRefPubMedGoogle Scholar
  5. Jia, B., Wu, Y., Li, B.Z., Mitchell, L.A., Liu, H., Pan, S., Wang, J., Zhang, H.R., Jia, N., Li, B., et al. (2018). Precise control of SCRaMbLE in synthetic haploid and diploid yeast. Nat Commun 9, 1933.CrossRefPubMedPubMedCentralGoogle Scholar
  6. Kannan, K., and Gibson, D.G. (2017). Yeast genome, by design. Science 355, 1024–1025.CrossRefPubMedGoogle Scholar
  7. Lena, H., Mitchell, L.A., Schulz, K., Messerschmidt, K., and Mueller-Roeber, B. (2018). L-SCRaMbLE as a tool for light-controlled Cremediated recombination in yeast. Nat Commun 9, 1931.CrossRefGoogle Scholar
  8. Liu, W., Luo, Z., Wang, Y., Pham, N., Tuck, L., Pi, I.P., Liu, L., Shen, Y., French, C., Auer, M., et al. (2018). Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods. Nat Commun 9, 1936.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Luo, Z., Wang, L., Wang, Y., Zhang, W., Guo, Y., Shen, Y., Jiang, L., Wu, Q., Zhang, C., Cai, Y., et al. (2018). Identifing and characterizing SCRaMbLEd synthetic yeast using ReSCuES. Nat Commun 9, 1930.CrossRefPubMedPubMedCentralGoogle Scholar
  10. Mitchell, L.A., Wang, A., Stracquadanio, G., Kuang, Z., Wang, X., Yang, K., Richardson, S., Martin, J.A., Zhao, Y., Walker, R., et al. (2017). Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond. Science 355, eaaf4831.CrossRefPubMedGoogle Scholar
  11. Richardson, S.M., Mitchell, L.A., Stracquadanio, G., Yang, K., Dymond, J. S., DiCarlo, J.E., Lee, D., Huang, C.L., Chandrasegaran, S., Cai, Y., et al. (2017). Design of a synthetic yeast genome. Science 355, 1040–1044.CrossRefPubMedGoogle Scholar
  12. Shen, M., Wu, Y., Yang, K., Li, Y., Xu, H., Zhang, H., Li, B., Li, X., Xiao, W.H., Xiao, Z., et al. (2018). Heterozygous diploid and interspecies SCRaMbLEing. Nat Commun 9, 1934.CrossRefPubMedPubMedCentralGoogle Scholar
  13. Shen, Y., Stracquadanio, G., Wang, Y., Yang, K., Mitchell, L.A., Xue, Y., Cai, Y., Chen, T., Dymond, J.S., Kang, K., et al. (2016). SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes. Genome Res 26, 36–49.CrossRefPubMedPubMedCentralGoogle Scholar
  14. Wu, Y., Zhu, R.Y., Mitchell, L.A., Ma, L., Liu, R., Zhao, M., Jia, B., Xu, H., Li, Y.X., Yang, Z.M., et al. (2018). In vitro DNA SCRaMbLE. Nat Commun 9, 1935.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Plant and Microbial BiologyUniversity of MinnesotaMinneapolisUSA
  2. 2.Department of Genetics, Cell Biology and DevelopmentUniversity of MinnesotaMinneapolisUSA
  3. 3.Center for Genome EngineeringUniversity of MinnesotaMinneapolisUSA
  4. 4.Center for Precision Plant GenomicsUniversity of MinnesotaMinneapolisUSA

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