GhKLCR1, a kinesin light chain-related gene, induces drought-stress sensitivity in Arabidopsis
- 154 Downloads
Drought stress results in significant losses in agricultural production, and especially that of cotton. The molecular mechanisms that coordinate drought tolerance remain elusive in cotton. Here, we isolated a drought-response gene GhKLCR1, which is a close homolog of AtKLCR1, which encodes a kinesin light chain-related protein enriched with a tetratrico peptide-repeat region. A subcellular localization assay showed that GhKLCR1 is associated with the cell membrane. A tissue-specific expression profile analysis demonstrated that GhKLCR1 is a cotton root-specific gene. Further abiotic and hormonal stress treatments showed that GhKLCR1 was upregulated during abiotic stresses, especially after polyethylene glycol treatments. In addition, the glucuronidase (GUS) staining activity increased as the increment of mannitol concentration in transgenic Arabidopsis plants harboring the fusion construct PGhKLCR1::GUS. The root lengths of 35S::GhKLCR1 lines were significantly reduced compared with that of wild type. Additionally, seed germination was strongly inhibited in 35S::GhKLCR1 lines after 300-mmol L−1 mannitol treatments as compared with Columbia-0, indicating the sensitivity of GhKLCR1 to drought. These findings provide a better understanding of the structural, physiological and functional mechanisms of kinesin light chain-related proteins.
KeywordsGhKLCR1 kinesin Arabidopsis mannitol seed germination drought sensitivity GUS cis-elements
Unable to display preview. Download preview PDF.
This work was supported by the National Natural Science Foundation of China (31501345).
- Bhatt, R.M., and Rao, N.K.S. (2005). Influence of pod load on response of okra to water stress. Indian J Plant Physiol 10, 54.Google Scholar
- Bürstenbinder, K., Savchenko, T., Müller, J., Adamson, A.W., Stamm, G., Kwong, R., Zipp, B.J., Dinesh, D.C., and Abel, S. (2013). Arabidopsis calmodulin-binding protein IQ67-domain 1 localizes to microtubules and interacts with kinesin light chain-related protein-1. J Biol Chem 288, 1871–1882.CrossRefGoogle Scholar
- Day, I.S., Reddy, V.S., Ali, G.S., and Reddy, A.S.N. (2002). Analysis of EF-hand-containing proteins in Arabidopsis. Genome Biol 3, research0056-1.Google Scholar
- Lin, Z., Arciga-Reyes, L., Zhong, S., Alexander, L., Hackett, R., Wilson, I., and Grierson, D. (2008). SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development. J Exp Bot 59, 4271–4287.CrossRefGoogle Scholar
- Mirza, M.A., and Sheikh, A.L. (1994). Prospects of developing novel cottons through biotechniques. Pakistan J Agr Res 15, 195–201.Google Scholar
- Nelson, D.E., Repetti, P.P., Adams, T.R., Creelman, R.A., Wu, J., Warner, D.C., Anstrom, D.C., Bensen, R.J., Castiglioni, P.P., Donnarummo, M. G., et al. (2007). Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci USA 104, 16450–16455.CrossRefGoogle Scholar
- Pace, P.F., Cralle, H.T., Shm, E.H., Cothren, J.T., and Senseman, S.A. (1999). Drought-induced changes in shoot and root growth of young cotton plants. J Cotton Sci 3, 183–187.Google Scholar
- Radin, J.W. (1981). Water relations of cotton plants under nitrogen deficiency. IV. Leaf senescence during drought and its relation to stomatal closure. Physiol Plant 51, 145–149.Google Scholar
- Skoufias, D.A., Cole, D.G., Wedaman, K.P., and Scholey, J.M. (1993). The caroxyl-terminal domain of kinesin heavy chain is important for membrane binding. J Biol Chem 269, 1477–1485.Google Scholar
- Yang, Z., Wolf, I.M., Chen, H., Periyasamy, S., Chen, Z., Yong, W., Shi, S., Zhao, W., Xu, J., Srivastava, A., et al. (2006). FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor A isoform. Mol Endocrinol 20, 2682–2694.CrossRefGoogle Scholar
- Zhang, T.Z., Hu, Y., Jiang, W.K., Fang, L., Guan, X.Y., Chen, J.D., Zhang, J.B., Saski, C.A., Scheffler, B.E., Stelly, D.M., et al. (2015). Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol 33, 531–537.Google Scholar
- Zhao, T.J., Sun, S., Liu, Y., Liu, J.M., Liu, Q., Yan, Y.B., and Zhou, H.M. (2006). Regulating the drought-responsive element (DRE)-mediated signaling pathway by synergic functions of trans-active and trans-inactive DRE binding factors in Brassica napus. J Biol Chem 281, 10752–10759.CrossRefGoogle Scholar